AB249493

Anti-Zmat2 antibody [EPR11136] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ZMAT2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Zinc finger matrin-type protein 2, ZMAT2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

This data was developed using ab169541, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling Zmat2 with ab169541 antibody at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

This data was developed using ab169541, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Zmat2 with ab169541 antibody at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

This data was developed using ab169541, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling Zmat2 with ab169541 at 1/250.

Unknown

Western blot - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

This data was developed using ab169541, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Zmat2 antibody [EPR11136] (<a href='/en-us/products/primary-antibodies/zmat2-antibody-epr11136-ab169541'>ab169541</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Unconjugated

Anti-Zmat2 antibody [EPR11136]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11136

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249493 is the carrier-free version of ab169541.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zmat2 also known as Zinc finger matrin-type protein 2 plays a role in RNA processing. This protein has a mass of approximately 37 kDa. It shows expression in most tissues with higher levels in certain areas of the brain and reproductive organs. The protein contains a zinc finger domain which facilitates binding to RNA and possibly other molecular components suggesting its involvement in cellular processes requiring precise regulation.

Biological function summary

Zmat2 influences the maturation and modification of mRNA. It serves as part of the spliceosome complex which is responsible for the splicing of pre-mRNA and is essential for the regulation of gene expression. Zmat2 contributes to the appropriate removal of introns and joining of exons ensuring the production of functional RNA transcripts. Its actions are important for the maintenance of cellular function and response to environmental cues.

Pathways

Several components in the RNA splicing machinery are associated with Zmat2. Among these the spliceosome assembly and mRNA surveillance pathways are notably significant. Zmat2 works in conjunction with PRPF8 and several small nuclear ribonucleoproteins (snRNPs) which are necessary for the assembly of the spliceosomal complex. These interactions ensure effective and reliable mRNA processing and turnover.

Abnormal functioning of Zmat2 may have a connection with certain neurological conditions. Disruption in RNA splicing can lead to the accumulation of faulty proteins potentially resulting in diseases like amyotrophic lateral sclerosis (ALS) and some neurodegenerative disorders. The involvement of Zmat2 in pathways with other proteins like FUS and TDP-43 which are linked to these conditions highlights its importance in maintaining neural health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in pre-mRNA splicing as a component of the spliceosome.

See full target information ZMAT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Zmat2 antibody [EPR11136] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

Immunocytochemistry/ Immunofluorescence - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

Western blot - Anti-Zmat2 antibody [EPR11136] - BSA and Azide free (AB249493)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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