Rabbit Polyclonal ZMPSTE24 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZMPSTE24 aa 200-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZMPSTE24 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link O75844
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Transmembrane metalloprotease whose catalytic activity is critical for processing lamin A/LMNA on the inner nuclear membrane and clearing clogged translocons on the endoplasmic reticulum (PubMed:33293369, PubMed:33315887). Proteolytically removes the C-terminal three residues of farnesylated proteins (PubMed:33293369, PubMed:33315887). Plays also an antiviral role independently of its protease activity by restricting enveloped RNA and DNA viruses, including influenza A, Zika, Ebola, Sindbis, vesicular stomatitis, cowpox, and vaccinia (PubMed:28169297, PubMed:28246125). Mechanistically, controls IFITM antiviral pathway to hinder viruses from breaching the endosomal barrier by modulating membrane fluidity (PubMed:35283811).
Alternative names
FACE1, STE24, ZMPSTE24, CAAX prenyl protease 1 homolog, Farnesylated proteins-converting enzyme 1, Prenyl protein-specific endoprotease 1, Zinc metalloproteinase Ste24 homolog, FACE-1
Recommended products
Rabbit Polyclonal ZMPSTE24 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZMPSTE24 aa 200-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZMPSTE24 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link O75844
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity <95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZMPSTE24 also known as FACE1 or STE24 is a zinc metalloprotease enzyme involved in the post-translational modification of prelamin A to mature lamin A. This enzyme specifically cleaves the C-terminal tail of prelamin A a critical step required for proper nuclear envelope structure and function. ZMPSTE24 has a molecular mass of approximately 54 kDa and is expressed in several tissues including skeletal muscle heart and skin. Its activity primarily takes place in the endoplasmic reticulum membrane where it executes its proteolytic functions.
- Biological function summary
The alteration of prelamin A to lamin A by ZMPSTE24 is necessary for maintaining nuclear shape and stability. Lamin A is a major component of the nuclear lamina providing structural support to the nuclear envelope. ZMPSTE24 is part of a proteolytic complex that ensures the proper maturation of lamin A which plays a significant role in DNA replication and cellular division. Deficiency in ZMPSTE24 activity can lead to the accumulation of farnesylated prelamin A disrupting nuclear architecture and cellular processes.
- Pathways
The function of ZMPSTE24 integrates into the broader context of the lamin A maturation pathway significant for nuclear integrity. It collaborates with other enzymes like farnesyltransferase and icmt during the maturation process of prelamin A to lamin A. ZMPSTE24 also interacts with the protein lamin B which is an associate in the nuclear lamina. This cooperation within the pathway ensures the functional execution of nuclear mechanical properties and DNA repair processes.
- Associated diseases and disorders
The abnormal function or mutation of ZMPSTE24 connects direly with disorders such as restrictive dermopathy and Hutchinson-Gilford progeria syndrome (HGPS). In HGPS defective ZMPSTE24 fails to process prelamin A leading to the build-up of a toxic version of prelamin A termed progerin. This accumulation contributes to premature aging phenotypes. The disorder correlates with mutations in LMNA a gene encoding lamins A and C highlighting the tandem dependency between ZMPSTE24 and lamin A in maintaining nuclear structure and overall cellular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMPSTE24 antibody (ab234832)
Paraffin-embedded human gastric cancer tissue stained for ZMPSTE24 using ab234832 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-ZMPSTE24 antibody (ab234832)
PC-3 (human prostate adenocarcinoma cell line) cells labeling ZMPSTE24 using ab234832 at 1/100 dilution in ICC/IF. Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMPSTE24 antibody (ab234832)
Paraffin-embedded human liver tissue stained for ZMPSTE24 using ab234832 at 1/100 dilution in immunohistochemical analysis.
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