Rabbit Recombinant Monoclonal ZNF136 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299), expandable thumbnail](https://content.abcam.com/products/images/znf136-antibody-epr8225-bsa-and-azide-free-ab248299--western-blot-img157098.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)")
![OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299), expandable thumbnail](https://content.abcam.com/products/images/znf136-antibody-epr8225-bsa-and-azide-free-ab248299--oi-rd-scanning-img154146.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Predicted | Not recommended | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
May be involved in transcriptional regulation as a weak repressor when alone, or a potent one when fused with a heterologous protein containing a KRAB B-domain.
Alternative names
Zinc finger protein 136, ZNF136
Recommended products
Rabbit Recombinant Monoclonal ZNF136 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8225
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 7 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248299 is the carrier-free version of Anti-ZNF136 antibody [EPR8225] ab129100.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNF136 also known as Zinc Finger Protein 136 functions as a transcription factor due to its zinc finger domains which allow it to bind specific DNA sequences. It weighs approximately 65 kDa. This protein regulates the expression of target genes by interacting with DNA in a sequence-specific manner. Scientists find it predominantly expressed in the brain with lower levels detected in other tissues suggesting a specialized role in neural processes.
- Biological function summary
ZNF136 regulates genes implicated in neuronal development and function. It may form part of transcriptional complexes but this requires further study. ZNF136 impacts the expression of genes necessary for maintaining neural network integrity and synaptic plasticity. Its activity supports proper cognitive function and neural communication indicating it plays a role in the regulation of brain homeostasis.
- Pathways
ZNF136 integrates into neural gene regulation networks. It influences the Wnt signaling pathway which is important in neuronal development and function and interacts with proteins such as Beta-Catenin. Additionally ZNF136 participates in the transcriptional regulation of genes within the Notch signaling pathway working with proteins like RBPJ to fine-tune responses required for cell differentiation and maturation.
- Associated diseases and disorders
ZNF136 has associations with neurological conditions like schizophrenia and autism spectrum disorders. Abnormal expression or mutations in ZNF136 may disrupt neural communication pathways contributing to disease pathogenesis. It also seems related to dysregulation of proteins like NRXN1 which is implicated in synaptic connectivity providing insight into how ZNF136 alterations could influence these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299), expandable thumbnail](https://content.abcam.com/products/images/znf136-antibody-epr8225-bsa-and-azide-free-ab248299--western-blot-img157098.jpg "Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299)")
Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299)
This data was developed using Anti-ZNF136 antibody [EPR8225] ab129100, the same antibody clone in a different buffer formulation.
Lane 1: Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299) at 1/1000 dilution
Lane 4: Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299)
Lane 1: Human fetal heart cell lysates at 10 µg
Lane 4: HeLa cell lysates
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 63 kDa
![OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299), expandable thumbnail](https://content.abcam.com/products/images/znf136-antibody-epr8225-bsa-and-azide-free-ab248299--oi-rd-scanning-img154146.jpg "OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299)")
OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
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- Visit the general protocols
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