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AB248299

Anti-ZNF136 antibody [EPR8225] - BSA and Azide free

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Rabbit Recombinant Monoclonal ZNF136 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Zinc finger protein 136, ZNF136

2 Images
Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)
  • WB

Unknown

Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)

This data was developed using ab129100, the same antibody clone in a different buffer formulation.

Lane 1:

Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299) at 1/1000 dilution

Lane 4:

Western blot - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (ab248299)

Lane 1:

Human fetal heart cell lysates at 10 µg

Lane 4:

HeLa cell lysates

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 63 kDa

false

OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ZNF136 antibody [EPR8225] - BSA and Azide free (AB248299)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8225

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248299 is the carrier-free version of ab129100.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF136 also known as Zinc Finger Protein 136 functions as a transcription factor due to its zinc finger domains which allow it to bind specific DNA sequences. It weighs approximately 65 kDa. This protein regulates the expression of target genes by interacting with DNA in a sequence-specific manner. Scientists find it predominantly expressed in the brain with lower levels detected in other tissues suggesting a specialized role in neural processes.
Biological function summary

ZNF136 regulates genes implicated in neuronal development and function. It may form part of transcriptional complexes but this requires further study. ZNF136 impacts the expression of genes necessary for maintaining neural network integrity and synaptic plasticity. Its activity supports proper cognitive function and neural communication indicating it plays a role in the regulation of brain homeostasis.

Pathways

ZNF136 integrates into neural gene regulation networks. It influences the Wnt signaling pathway which is important in neuronal development and function and interacts with proteins such as Beta-Catenin. Additionally ZNF136 participates in the transcriptional regulation of genes within the Notch signaling pathway working with proteins like RBPJ to fine-tune responses required for cell differentiation and maturation.

ZNF136 has associations with neurological conditions like schizophrenia and autism spectrum disorders. Abnormal expression or mutations in ZNF136 may disrupt neural communication pathways contributing to disease pathogenesis. It also seems related to dysregulation of proteins like NRXN1 which is implicated in synaptic connectivity providing insight into how ZNF136 alterations could influence these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation as a weak repressor when alone, or a potent one when fused with a heterologous protein containing a KRAB B-domain.
See full target information ZNF136
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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