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AB112058

Anti-ZNF148 antibody

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(1 Publication)

Rabbit Polyclonal ZNF148 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZNF148 aa 700 to C-terminus.

View Alternative Names

ZBP89, ZNF148, Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89

1 Images
Western blot - Anti-ZNF148 antibody (AB112058)
  • WB

Unknown

Western blot - Anti-ZNF148 antibody (AB112058)

All lanes:

Western blot - Anti-ZNF148 antibody (ab112058) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg/mL

Lane 2:

HeLa whole cell lysate at 15 µg/mL

Lane 3:

HeLa whole cell lysate at 5 µg/mL

Lane 4:

293T whole cell lysate at 50 µg/mL

Lane 5:

Mouse NIH3T3 whole cell lysate at 50 µg/mL

Predicted band size: 89 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ZNF148 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UQR1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF148 also known as ZBP-89 or BFCOL1 is a zinc finger transcription factor involved in the regulation of gene expression. The protein has a molecular mass of approximately 80 kDa. It is found in a range of tissues but shows higher expression levels in the liver and colon. ZNF148 binds to the GC-rich sequences in the promoter regions thereby influencing transcriptional activities of its target genes.
Biological function summary

ZNF148 plays a role in the regulation of cell growth and differentiation. It does not form part of a multi-protein complex but interacts directly with other transcription factors and the basal transcription machinery. The protein can repress or activate transcription depending on the cellular context and specific gene target. ZNF148 has key roles in processes such as regulation of apoptosis and maintenance of tissue homeostasis.

Pathways

ZNF148 significantly impacts the Wnt signaling pathway contributing to cell proliferation and differentiation. It also has involvement in the p53 network where it regulates genes critical for cell cycle arrest and apoptosis. It involves proteins like β-catenin in the Wnt pathway and it influences the p53 protein within the cell cycle pathway.

ZNF148 has links to colorectal cancer and liver diseases. In colorectal cancer its dysregulation can affect cell proliferation and apoptosis through interactions with proteins such as β-catenin. In liver diseases alterations in ZNF148 expression impact the balance of cell survival and death with connections to the p53 protein playing an important part in liver tissue response to stress and damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
See full target information ZNF148
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 44:72-85 PubMed38482865

2024

ANP32e Binds Histone H2A.Z in a Cell Cycle-Dependent Manner and Regulates Its Protein Stability in the Cytoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Dijkwel,Gene Hart-Smith,Sebastian Kurscheid,David J Tremethick
View all publications
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Product promise

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