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AB227076

Anti-ZNF148 antibody

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(1 Publication)

Rabbit Polyclonal ZNF148 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF148.

View Alternative Names

ZBP89, ZNF148, Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZNF148 antibody (AB227076)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZNF148 antibody (AB227076)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNF148 (green) using ab227076 at1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF148 antibody (AB227076)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF148 antibody (AB227076)

Paraffin-embedded human breast cancer tissue stained for ZNF148 with ab227076 at 1/250 dilution in immunohistochemical analysis.

Western blot - Anti-ZNF148 antibody (AB227076)
  • WB

Supplier Data

Western blot - Anti-ZNF148 antibody (AB227076)

All lanes:

Western blot - Anti-ZNF148 antibody (ab227076) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 89 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZNF148. The exact immunogen used to generate this antibody is proprietary information.

Q9UQR1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF148 also known as ZBP-89 or BFCOL1 is a zinc finger transcription factor involved in the regulation of gene expression. The protein has a molecular mass of approximately 80 kDa. It is found in a range of tissues but shows higher expression levels in the liver and colon. ZNF148 binds to the GC-rich sequences in the promoter regions thereby influencing transcriptional activities of its target genes.
Biological function summary

ZNF148 plays a role in the regulation of cell growth and differentiation. It does not form part of a multi-protein complex but interacts directly with other transcription factors and the basal transcription machinery. The protein can repress or activate transcription depending on the cellular context and specific gene target. ZNF148 has key roles in processes such as regulation of apoptosis and maintenance of tissue homeostasis.

Pathways

ZNF148 significantly impacts the Wnt signaling pathway contributing to cell proliferation and differentiation. It also has involvement in the p53 network where it regulates genes critical for cell cycle arrest and apoptosis. It involves proteins like β-catenin in the Wnt pathway and it influences the p53 protein within the cell cycle pathway.

ZNF148 has links to colorectal cancer and liver diseases. In colorectal cancer its dysregulation can affect cell proliferation and apoptosis through interactions with proteins such as β-catenin. In liver diseases alterations in ZNF148 expression impact the balance of cell survival and death with connections to the p53 protein playing an important part in liver tissue response to stress and damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
See full target information ZNF148
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5411-5427 PubMed40959276

2025

Therapeutic Activation of PPARα Inhibits Transformed Follicular Lymphoma Tumorigenesis via the FOXM1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Qin,Hui Zhou,Jie Zhou,Chendi Xie,Shuman Jia,Xingxing Yu,Yan Hong,Li Zhang,Yueting Huang,Yong Zhou,Bing Xu,Jie Zha
View all publications
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Product promise

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