AB69933

Anti-ZNF148 antibody

Name: Anti-ZNF148 antibody (ab69933) | Abcam
Brand: Abcam Limited
SKU: AB69933
Price: 470 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(10 Publications)

Rabbit Polyclonal ZNF148 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human ZNF148.

View Alternative Names

ZBP89, ZNF148, Zinc finger protein 148, Transcription factor ZBP-89, Zinc finger DNA-binding protein 89

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF148 antibody (AB69933)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF148 antibody (AB69933)

Immunohistochemical analysis of paraffin embedded human brain tissue using ab69933 at 1/50 dilution. Right panel was treated with immunising peptide.

Unknown

Western blot - Anti-ZNF148 antibody (AB69933)

All lanes:

Western blot - Anti-ZNF148 antibody (ab69933) at 1/500 dilution

Lane 1:

HepG2 cell extract at 30 µg

Lane 2:

HepG2 cell extract at 30 µg with immunising peptide

Predicted band size: 89 kDa

Observed band size: 89 kDa,95 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ZNF148. The exact immunogen used to generate this antibody is proprietary information.

Q9UQR1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF148 also known as ZBP-89 or BFCOL1 is a zinc finger transcription factor involved in the regulation of gene expression. The protein has a molecular mass of approximately 80 kDa. It is found in a range of tissues but shows higher expression levels in the liver and colon. ZNF148 binds to the GC-rich sequences in the promoter regions thereby influencing transcriptional activities of its target genes.

Biological function summary

ZNF148 plays a role in the regulation of cell growth and differentiation. It does not form part of a multi-protein complex but interacts directly with other transcription factors and the basal transcription machinery. The protein can repress or activate transcription depending on the cellular context and specific gene target. ZNF148 has key roles in processes such as regulation of apoptosis and maintenance of tissue homeostasis.

Pathways

ZNF148 significantly impacts the Wnt signaling pathway contributing to cell proliferation and differentiation. It also has involvement in the p53 network where it regulates genes critical for cell cycle arrest and apoptosis. It involves proteins like β-catenin in the Wnt pathway and it influences the p53 protein within the cell cycle pathway.

ZNF148 has links to colorectal cancer and liver diseases. In colorectal cancer its dysregulation can affect cell proliferation and apoptosis through interactions with proteins such as β-catenin. In liver diseases alterations in ZNF148 expression impact the balance of cell survival and death with connections to the p53 protein playing an important part in liver tissue response to stress and damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.

See full target information ZNF148

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 12:2582-2595 PubMed37969391

2023

The transcription activity of on expression is significantly blocked by methylation of CpG shore in non-promoter of lung cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Honghao Peng,Wenfan Fu,Chong Chang,Huan Gao,Qianmei He,Ziqi Liu,Mengxing Cui,Han Wang,Yongjiang Yu,Yonghui Wu,Xue Zhang,Shuyun Jiang,Chi Xu,Xiaoyu Shen,Zhihan Zhang,Yixiang Zhou,Daochuan Li,Qing Wang

Computational and mathematical methods in medicine 2022:4622877 PubMed35111235

2022

ZNF488 Promotes the Invasion and Migration of Pancreatic Carcinoma Cells through the Akt/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongquan Qiu,Liang Zhang,Dongzhi Wang,Yu Zhang,Hongyu Cai,Haiyan Miao,Feihu Chu

Cancer science 111:1266-1278 PubMed32060966

2020

Upregulation of ZNF148 in SDHB-deficient gastrointestinal stromal tumor potentiates Forkhead box M1-mediated transcription and promotes tumor cell invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Gao,Chunmin Ma,Xiangwei Sun,Qin Zhao,Yong Fang,Yuhui Jiang,Kuntang Shen,Xian Shen

Physiological reports 7:e14058 PubMed31025534

2019

Klf4, Klf2, and Zfp148 activate autophagy-related genes in smooth muscle cells during aortic aneurysm formation.

Applications

Unspecified application

Species

Unspecified reactive species

Morgan Salmon,Michael Spinosa,Zendra E Zehner,Gilbert R Upchurch,Gorav Ailawadi

Arteriosclerosis, thrombosis, and vascular biology 39:73-88 PubMed30580567

2018

ZFP148 (Zinc-Finger Protein 148) Binds Cooperatively With NF-1 (Neurofibromin 1) to Inhibit Smooth Muscle Marker Gene Expression During Abdominal Aortic Aneurysm Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Morgan Salmon,Basil Schaheen,Michael Spinosa,William Montgomery,Nicolas H Pope,John P Davis,William F Johnston,Ashish K Sharma,Gary K Owens,Juanita L Merchant,Zendra E Zehner,Gilbert R Upchurch,Gorav Ailawadi

Medicine 96:e5845 PubMed28072746

2017

ZNF148 modulates TOP2A expression and cell proliferation via ceRNA regulatory mechanism in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Hua Gao,Juan Li,Yan Liu,Qi Zhi Liu,Li Qiang Hao,Lian Jie Liu,Wei Zhang

World journal of gastroenterology 21:8836-47 PubMed26269673

2015

Upregulation of nemo-like kinase is an independent prognostic factor in colorectal cancer.

Applications

IHC-P

Species

Human

Wei Zhang,Jian He,Yan Du,Xian-Hua Gao,Yan Liu,Qi-Zhi Liu,Wen-Jun Chang,Guang-Wen Cao,Chuan-Gang Fu

Cell reports 4:589-600 PubMed23911284

2013

Locus-specific proteomics by TChP: targeted chromatin purification.

Applications

Species

Human

Farzin Pourfarzad,Ali Aghajanirefah,Ernie de Boer,Sara Ten Have,Thamar Bryn van Dijk,Sima Kheradmandkia,Ralph Stadhouders,Supat Thongjuea,Eric Soler,Nynke Gillemans,Marieke von Lindern,Jeroen Demmers,Sjaak Philipsen,Frank Grosveld

Cancer 119:2212-22 PubMed23576061

2013

Expression of ZNF148 in different developing stages of colorectal cancer and its prognostic value: a large Chinese study based on tissue microarray.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Hua Gao,Qi-Zhi Liu,Wenjun Chang,Xiao-Dong Xu,Yan Du,Yifang Han,Yan Liu,Zhi-Qi Yu,Zhi-Gui Zuo,Jun-Jie Xing,Guangwen Cao,Chuan-Gang Fu

Molecular & cellular proteomics : MCP 11:1263-73 PubMed22872859

2012

Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.

Applications

Species

Mouse

Pavlos Fanis,Nynke Gillemans,Ali Aghajanirefah,Farzin Pourfarzad,Jeroen Demmers,Fatemehsadat Esteghamat,Ratna K Vadlamudi,Frank Grosveld,Sjaak Philipsen,Thamar B van Dijk

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF148 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF148 antibody (AB69933)

Western blot - Anti-ZNF148 antibody (AB69933)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise