AB175227

Anti-ZNF160 antibody [EPR12523]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ZNF160 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1611, ZNF160, Zinc finger protein 160, Zinc finger protein HZF5, Zinc finger protein Kr18, HKr18

3 Images

Flow Cytometry (Intracellular) - Anti-ZNF160 antibody [EPR12523] (AB175227)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZNF160 antibody [EPR12523] (AB175227)

Intracellular flow cytometric analysis of permeabilized U87-MG cells labeling ZNF160with ab175227 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Supplier Data

Immunoprecipitation - Anti-ZNF160 antibody [EPR12523] (AB175227)

Western blot analysis on immunoprecipitation pellet from SW480 cell lysate using ab175227.

All lanes:

Immunoprecipitation - Anti-ZNF160 antibody [EPR12523] (ab175227)

Predicted band size: 94 kDa

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Supplier Data

Western blot - Anti-ZNF160 antibody [EPR12523] (AB175227)

All lanes:

Western blot - Anti-ZNF160 antibody [EPR12523] (ab175227) at 1/1000 dilution

Lane 1:

SW480 cell lysate at 10 µg

Lane 2:

Caco-2 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Lane 5:

Fetal liver cell lysate at 10 µg

Predicted band size: 94 kDa

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Carrier free

Anti-ZNF160 antibody [EPR12523] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12523

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF160 also known as Zinc Finger Protein 160 is a protein encoded by the ZNF160 gene. It functions as a transcription factor primarily involved in binding to specific DNA sequences to regulate the transcription of genetic information from DNA to mRNA. The molecular mass of ZNF160 is approximately 90 kDa. This protein is predominantly expressed in the nucleus of cells with high expression levels noted in hematopoietic tissues including bone marrow and lymphoid tissues.

Biological function summary

ZNF160 plays an important role in regulating gene expression associated with cellular growth and differentiation. It forms part of multi-protein complexes that exert control over the transcriptional machinery. By modulating the transcription of various genes ZNF160 contributes to the maintenance of normal cellular processes. Its regulatory activities are important in ensuring that cell division and development proceed correctly and it may act as a suppressor or activator depending on the cellular context.

Pathways

ZNF160 engages in pivotal roles within the Wnt signaling pathway and Notch signaling pathway. These pathways are integral to cell fate determination and embryonic development. In the Wnt signaling pathway associations with proteins like β-catenin enable ZNF160 to influence gene expression related to cell cycle progression. Interactions with the Notch signaling pathway involve collaboration with proteins such as Notch1 affecting differentiation processes in various cell types.

ZNF160 has been implicated in certain cancers and developmental disorders. Its dysregulation can lead to aberrant cell proliferation contributing to cancerous growths. ZNF160 in conjunction with proteins associated with cancer progression such as p53 may influence tumor suppression or oncogenic pathways. In developmental disorders mutations or altered expression of ZNF160 lead to defects in cellular differentiation resulting in conditions like congenital malformations. The link between ZNF160 and these disorders highlights the significance of proper regulatory mechanisms in maintaining health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in transcriptional regulation.

See full target information ZNF160

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Stem cells (Dayton, Ohio) 42:861-873 PubMed39169713

2024

Integrin-linked kinase control dental pulp stem cell senescence via the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Xiping Wang,Sha Tian,Linxi Zhou,Li Wang,Xiaohan Liu,Zihan Yang,Guiqiang Fu,Xingguang Liu,Chen Ding,Duohong Zou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF160 antibody [EPR12523]

Flow Cytometry (Intracellular) - Anti-ZNF160 antibody [EPR12523] (AB175227)

Immunoprecipitation - Anti-ZNF160 antibody [EPR12523] (AB175227)

Western blot - Anti-ZNF160 antibody [EPR12523] (AB175227)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise