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Rabbit Recombinant Monoclonal ZNF175 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (AB249172), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (AB249172), expandable thumbnail
  • Western blot - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (AB249172), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Expected
Not recommended
Mouse
Expected
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Target data

Function

Down-regulates the expression of several chemokine receptors. Interferes with HIV-1 replication by suppressing Tat-induced viral LTR promoter activity.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ZNF175 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR8391(2)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab249172 is the carrier-free version of Anti-ZNF175 antibody [EPR8391(2)] ab155075.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ZNF175 also known as Zinc Finger Protein 175 is part of the Krüppel-type zinc finger protein family. It weighs around 56-58 kDa and functions as a transcription factor meaning it binds to specific DNA sequences to regulate the transcription of genetic information. ZNF175 is typically expressed in various tissues with higher expression observed in the brain and reproductive tissues. The zinc finger motifs in its structure allow ZNF175 to interact directly with DNA influencing the transcriptional activity of specific genes.

Biological function summary

The protein's role centers around gene regulation and control of developmental processes. ZNF175 doesn't form elaborate complexes; instead it acts more independently or with few partners during transcriptional regulation. It can influence the expression of genes involved in cellular growth and differentiation impacting the cellular phenotype. The presence of multiple zinc fingers enhances its ability to target specific DNA sequences with high affinity affecting downstream gene expression in a targeted manner.

Pathways

Researchers have found ZNF175 involved in the regulation of developmental and cellular proliferation pathways. Its activity impacts the Hedgehog signaling pathway an important regulator of embryogenesis and adult tissue homeostasis. ZNF175 may interact with proteins such as glioma-associated oncogene (GLI) transcription factors to modulate this pathway's output. The involvement of ZNF175 in these pathways highlights its significant influence in maintaining cellular communication and growth balance.

Associated diseases and disorders

Changes in ZNF175 expression levels have connections to some neurodegenerative conditions. Aberrant expression of ZNF175 has a reported association with Alzheimer's disease possibly influencing genes that regulate neuronal survival and function. Additionally in the context of cancer altered ZNF175 expression might impact tumorigenesis through pathways linked to proteins such as GLI contributing to the uncontrolled cell proliferation characteristic of cancerous tissues. Understanding ZNF175's role in these diseases helps in developing targeted therapeutic approaches.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (ab249172), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (ab249172)

    This data was developed using Anti-ZNF175 antibody [EPR8391(2)] ab155075, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling ZNF175 with Anti-ZNF175 antibody [EPR8391(2)] ab155075 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (ab249172), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (ab249172)

    This data was developed using Anti-ZNF175 antibody [EPR8391(2)] ab155075, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, labeling ZNF175 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (ab249172), expandable thumbnail

    Western blot - Anti-ZNF175 antibody [EPR8391(2)] - BSA and Azide free (ab249172)

    This data was developed using Anti-ZNF175 antibody [EPR8391(2)] ab155075, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ZNF175 antibody [EPR8391(2)] (Anti-ZNF175 antibody [EPR8391(2)] ab155075) at 1/100 dilution

    Lane 1: Neuro-2a cell lysate at 10 µg

    Lane 2: SH-SY5Y cell lysate at 10 µg

    Lane 3: 293T cell lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Predicted band size: 82 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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