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AB228926

Anti-ZNF207/BUGZ antibody

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(1 Publication)

Rabbit Polyclonal ZNF207/BUGZ antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF207.

View Alternative Names

BUGZ, ZNF207, BUB3-interacting and GLEBS motif-containing protein ZNF207, BuGZ, hBuGZ, Zinc finger protein 207

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZNF207/BUGZ antibody (AB228926)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZNF207/BUGZ antibody (AB228926)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNF207/BUGZ(green) using ab228926 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF207/BUGZ antibody (AB228926)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF207/BUGZ antibody (AB228926)

Paraffin-embedded human colon carcinoma tissue stained for ZNF207/BUGZ using ab228926 at 1/250 dilution in immunohistochemical analysis.

Western blot - Anti-ZNF207/BUGZ antibody (AB228926)
  • WB

Supplier Data

Western blot - Anti-ZNF207/BUGZ antibody (AB228926)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-ZNF207/BUGZ antibody (ab228926) at 1/1000 dilution

All lanes:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZNF207. The exact immunogen used to generate this antibody is proprietary information.

O43670

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF207 also known as BUGZ functions as a zinc finger protein involved in regulating microtubule dynamics. The molecular weight of ZNF207/BUGZ is around 90 kDa. It finds expression widely in human tissues but shows a higher abundance in cells actively undergoing division. This protein assists in stabilizing spindle microtubules by promoting the proper assembly of tubulin subunits which is essential during mitosis. Additionally ZNF207 appears significant in maintaining genomic stability acting as a safeguard for accurate chromosome segregation.
Biological function summary

ZNF207/BUGZ plays a critical role in mitotic progression and spindle stability. This protein integrates into the spindle assembly checkpoint complex ensuring fidelity during mitotic chromosome segregation. By interacting with BUB3 a spindle checkpoint protein ZNF207/BUGZ facilitates the recruitment and localization of key components to the mitotic spindle during cell division. Furthermore its involvement in thymocyte differentiation highlights its importance in immune cell development.

Pathways

The mechanism of ZNF207/BUGZ intersects with the cell cycle regulatory network and the microtubule organization pathway. In the cell cycle pathway it influences the control of mitosis in sync with proteins like MAD2 and BUBR1 which are important for checkpoint signaling and prevention of premature anaphase onset. In terms of microtubule dynamics its interaction with tubulin ensures that proper spindle architecture and tension are maintained throughout the mitotic process supporting the necessary stability for successful completion of cell division.

Dysregulation of ZNF207/BUGZ activity connects to cancer where abnormal expression or mislocalization leads to chromosomal instability a hallmark of many tumor types. Research also links ZNF207/BUGZ abnormalities to neurodegenerative disorders though details remain under investigation. In these contexts its interaction with proteins like TP53 in cancer or TAU in neurodegenerative conditions could provide insights into potential therapeutic strategies or biomarkers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed : 24462186, PubMed : 24462187, PubMed : 26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed : 26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed : 24462186, PubMed : 24462187). Mechanisms by which BUB3 is protected are unclear : according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed : 24462186). According to another report, the stabilization is independent of the proteasome (PubMed : 24462187).
See full target information ZNF207
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:557-576 PubMed37938170

2023

ATG9B is a tissue-specific homotrimeric lipid scramblase that can compensate for ATG9A.

Applications

Unspecified application

Species

Unspecified reactive species

George N Chiduza,Acely Garza-Garcia,Eugenia Almacellas,Stefano De Tito,Valerie E Pye,Alexander R van Vliet,Peter Cherepanov,Sharon A Tooze
View all publications
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Product promise

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