Rabbit Polyclonal ZNF207/BUGZ antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF207.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Cow | Predicted | Predicted | Predicted |
Rhesus monkey | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly (PubMed:24462186, PubMed:24462187, PubMed:26388440). ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3 (PubMed:24462186, PubMed:24462187). Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3 (PubMed:24462186). According to another report, the stabilization is independent of the proteasome (PubMed:24462187).
BUGZ, ZNF207, BUGZ, BUB3-interacting and GLEBS motif-containing protein ZNF207, BuGZ, hBuGZ, Zinc finger protein 207
Rabbit Polyclonal ZNF207/BUGZ antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF207.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
ZNF207 also known as BUGZ functions as a zinc finger protein involved in regulating microtubule dynamics. The molecular weight of ZNF207/BUGZ is around 90 kDa. It finds expression widely in human tissues but shows a higher abundance in cells actively undergoing division. This protein assists in stabilizing spindle microtubules by promoting the proper assembly of tubulin subunits which is essential during mitosis. Additionally ZNF207 appears significant in maintaining genomic stability acting as a safeguard for accurate chromosome segregation.
ZNF207/BUGZ plays a critical role in mitotic progression and spindle stability. This protein integrates into the spindle assembly checkpoint complex ensuring fidelity during mitotic chromosome segregation. By interacting with BUB3 a spindle checkpoint protein ZNF207/BUGZ facilitates the recruitment and localization of key components to the mitotic spindle during cell division. Furthermore its involvement in thymocyte differentiation highlights its importance in immune cell development.
The mechanism of ZNF207/BUGZ intersects with the cell cycle regulatory network and the microtubule organization pathway. In the cell cycle pathway it influences the control of mitosis in sync with proteins like MAD2 and BUBR1 which are important for checkpoint signaling and prevention of premature anaphase onset. In terms of microtubule dynamics its interaction with tubulin ensures that proper spindle architecture and tension are maintained throughout the mitotic process supporting the necessary stability for successful completion of cell division.
Dysregulation of ZNF207/BUGZ activity connects to cancer where abnormal expression or mislocalization leads to chromosomal instability a hallmark of many tumor types. Research also links ZNF207/BUGZ abnormalities to neurodegenerative disorders though details remain under investigation. In these contexts its interaction with proteins like TP53 in cancer or TAU in neurodegenerative conditions could provide insights into potential therapeutic strategies or biomarkers.
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10% SDS-PAGE gel.
All lanes: Western blot - Anti-ZNF207/BUGZ antibody (ab228926) at 1/1000 dilution
All lanes: A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 51 kDa
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNF207/BUGZ(green) using ab228926 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Paraffin-embedded human colon carcinoma tissue stained for ZNF207/BUGZ using ab228926 at 1/250 dilution in immunohistochemical analysis.
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