Rabbit Recombinant Monoclonal ZNF217 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] - BSA and Azide free (AB240008), expandable thumbnail](https://content.abcam.com/products/images/znf217-antibody-epr6362-bsa-and-azide-free-ab240008--oi-rd-scanning-img82362.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] - BSA and Azide free (AB240008)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
Alternative names
ZABC1, ZNF217, Zinc finger protein 217
Recommended products
Rabbit Recombinant Monoclonal ZNF217 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6362
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 9.3 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240008 is the carrier-free version of Anti-ZNF217 antibody [EPR6362] ab124927.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNF217 also known as Zinc Finger Protein 217 functions mechanically as a transcriptional repressor and is part of the Krüppel-like C2H2 zinc finger proteins. It has a molecular mass of approximately 121 kDa. ZNF217 is primarily expressed in tissues such as the brain prostate and breast. It binds to specific DNA sequences and regulates gene expression often altering cellular differentiation and proliferation by repressing or activating target genes.
- Biological function summary
ZNF217 plays a significant role in cellular processes such as cell growth and survival. The protein associates with a multiprotein complex that includes CoREST histone deacetylases and lysine-specific demethylases. This association impacts chromatin remodeling and transcriptional control. ZNF217's influence on gene expression affects important cellular functions including differentiation and apoptosis by modifying chromatin structure and subsequently regulating gene transcription levels.
- Pathways
This transcriptional regulator influences two critical signaling pathways the TGF-beta and PI3K/AKT pathways both of which are essential for cellular growth and survival. ZNF217 interacts with proteins like SMAD2 which is a part of the TGF-beta pathway affecting cellular proliferation and differentiation processes. Through these connections ZNF217 contributes to the modulation of signaling cascades that control various aspects of cell behavior often with direct impacts on cellular homeostasis and pathology.
- Associated diseases and disorders
Altered expression of ZNF217 associates with several cancers including breast cancer and ovarian cancer. Overexpression of ZNF217 correlates with the progression and poor prognosis of these cancers. This zinc finger protein interacts with MYC a well-known regulator involved in many cancers promoting cellular proliferation and survival in oncogenic contexts. These interactions illustrate ZNF217's role in tumorigenesis and highlight its potential as a target for therapeutic interventions in cancer treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] - BSA and Azide free (ab240008), expandable thumbnail](https://content.abcam.com/products/images/znf217-antibody-epr6362-bsa-and-azide-free-ab240008--oi-rd-scanning-img82362.jpg "OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] - BSA and Azide free (ab240008)")
OI-RD Scanning - Anti-ZNF217 antibody [EPR6362] - BSA and Azide free (ab240008)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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