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AB241956

Anti-ZNF295 antibody

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(1 Publication)

Rabbit Polyclonal ZNF295 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZBTB21 aa 1000 to C-terminus.

View Alternative Names

KIAA1227, ZNF295, ZBTB21, Zinc finger and BTB domain-containing protein 21, Zinc finger protein 295

2 Images
Immunoprecipitation - Anti-ZNF295 antibody (AB241956)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZNF295 antibody (AB241956)

ZNF295 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241956 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab241956 at 1 μg/ml.

Lane 1 : ab241956 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZNF295 antibody (ab241956)

Predicted band size: 119 kDa

false

Western blot - Anti-ZNF295 antibody (AB241956)
  • WB

Supplier Data

Western blot - Anti-ZNF295 antibody (AB241956)

All lanes:

Western blot - Anti-ZNF295 antibody (ab241956) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 119 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ZBTB21 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9ULJ3

Specificity

Antibody was affinity purified using an epitope specific to ZNF295 immobilized on solid support.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab241956 was affinity purified using an epitope specific to ZNF295 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF295 also known as zinc finger protein 295 is a transcription factor that contains a zinc finger domain enabling interaction with DNA. It has an approximate mass of 115 kDa. ZNF295 is expressed in various tissues including brain liver and heart where it may control gene expression by binding specific DNA sequences. Its role in gene regulation indicates a significant impact on cellular processes influencing development and cellular differentiation.
Biological function summary

This transcription factor interacts with other proteins to modulate gene expression by forming complexes. It participates in regulating genes involved in cell growth and development. The zinc finger domain allows ZNF295 to bind DNA acting as a switch to activate or repress transcription. Through its activity it contributes to maintaining normal cellular function and responding to environmental changes which is essential for organismal homeostasis.

Pathways

ZNF295 plays roles in key signaling pathways such as the cell growth and differentiation pathways. It interacts with other transcription factors and proteins including members of the p53 family which are important in managing cell cycle and apoptosis. This involvement positions ZNF295 as a potential regulator of various cellular activities modulating the balance between cell survival and programmed cell death.

Mutations and altered expression of ZNF295 have connections to cancer and neurodevelopmental disorders. Disruptions in its function can lead to abnormal cell proliferation and differentiation conditions often observed in cancers. In neurodevelopmental disorders ZNF295 may play a role alongside proteins such as MECP2 in brain development anomalies. Understanding its contributions to these diseases allows for the exploration of targeted therapies that may modify its activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcription repressor.
See full target information ZBTB21
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed33079728

2020

Guanosine triphosphate links MYC-dependent metabolic and ribosome programs in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Huang,Kenneth E Huffman,Zixi Wang,Xun Wang,Kailong Li,Feng Cai,Chendong Yang,Ling Cai,Terry S Shih,Lauren G Zacharias,Andrew Chung,Qian Yang,Milind D Chalishazar,Abbie S Ireland,C Allison Stewart,Kasey Cargill,Luc Girard,Yi Liu,Min Ni,Jian Xu,Xudong Wu,Hao Zhu,Benjamin Drapkin,Lauren A Byers,Trudy G Oliver,Adi F Gazdar,John D Minna,Ralph J DeBerardinis
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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