AB202965

Anti-ZNF300 antibody

Name: Anti-ZNF300 antibody (ab202965) | Abcam
Brand: Abcam Limited
SKU: AB202965

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(2 Publications)

Rabbit Polyclonal ZNF300 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ZNF300 aa 150-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Zinc finger protein 300, ZNF300

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF300 antibody (AB202965)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF300 antibody (AB202965)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat brain tissue labeling ZNF300 with ab202965 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-ZNF300 antibody (AB202965)

12% Gel.

All lanes:

Western blot - Anti-ZNF300 antibody (ab202965) at 1/200 dilution

Lane 1:

Rat brain tissue lysate

Lane 2:

Mouse intestine tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

Predicted band size: 69 kDa

Observed band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ZNF300 aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96RE9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Use at 1/50 - 1/200 with fluorescent detection methods. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF300 also known as Zinc Finger Protein 300 is a DNA-binding protein with a molecular weight of approximately 86 kDa. It belongs to the zinc finger protein family which usually functions as transcription factors. ZNF300 expresses in various tissues including the heart kidney and liver. This protein can bind specific DNA sequences to regulate gene expression impacting many cellular functions.

Biological function summary

This protein plays a role in the regulation of transcription linked to cellular growth and development. ZNF300 might act as a part of larger protein complexes that control specific gene expression. Although comprehensive biological roles of ZNF300 remain under study current research suggests its involvement in cellular proliferation and anti-apoptotic processes indicating its contribution to cell survival mechanisms.

Pathways

ZNF300 is associated with cellular growth signaling and apoptosis regulation pathways. Its activity relates to pathways like the MAPK signaling pathway where it might interact with proteins such as ERK and p38 which are important for mediating responses to growth factors and stress. The protein's influence on gene transcription links it indirectly to other transcriptional regulators involved in these pathways.

Research identifies potential connections between ZNF300 and oncogenesis. Studies implicate its altered expression with tumorigenesis notably in liver cancer where it might work with proteins such as p53 a critical tumor suppressor. Additional evidence suggests a relationship with neurodegenerative disorders pointing to its influence on neural cell survival and function perhaps involving proteins like Bcl-2 that regulate apoptosis in neurons.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has a transcriptional repressor activity.

See full target information ZNF300

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 13:986-996 PubMed35166026

2022

Cisplatin resistance and malignant behaviors of lung cancer cells are promoted by circ_0002360 via targeting miR-6751-3p to regulate the expression of ZNF300.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyan Ding,Lizhi Li,Zhaohui Tang

European journal of nutrition 59:2497-2506 PubMed31570976

2019

Maternal high-protein diet modulates hepatic growth axis in weaning piglets by reprogramming the IGFBP-3 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Rihua Cong,Xiaoli Qu,Hui Zhang,Yongling Hu,Silin Ye,Demin Cai,Xian Li,Hao-Yu Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF300 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF300 antibody (AB202965)

Western blot - Anti-ZNF300 antibody (AB202965)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise