AB69436

Anti-ZNF312/FEZF2 antibody

Name: Anti-ZNF312/FEZF2 antibody. Rabbit polyclonal (ab69436) | Abcam
Brand: Abcam Limited
SKU: AB69436
Price: 425 USD
Availability: InStock
Rating: 3 (4 reviews)

(4 Reviews)

(5 Publications)

Rabbit Polyclonal ZNF312/FEZF2 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 5 publications.

View Alternative Names

Fez, Fezl, Zfp312, Fezf2, Fez family zinc finger protein 2, Forebrain embryonic zinc finger-like protein 2, Zinc finger protein 312, Zinc finger protein Fez-like

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (AB69436)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (AB69436)

ab69436 staining Fezf2 in TRA-differentiated Neuro2a. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab69436 at 1/1000 dilution and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). The secondary antibody (shown in green) was ab150081 Alexa Fluor^® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Unknown

Western blot - Anti-ZNF312/FEZF2 antibody (AB69436)

All lanes:

Western blot - Anti-ZNF312/FEZF2 antibody (ab69436) at 1 µg/mL

All lanes:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 49 kDa

Observed band size: 17 kDa,33 kDa,48 kDa

true

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF312 also known as FEZF2 (Forebrain Embryonic Zinc Finger-Like Protein 2) is a transcription factor with a mass of approximately 68 kDa. This protein features zinc finger motifs that interact with specific DNA sequences. ZNF312/FEZF2 promotes transcriptional regulation by binding to DNA and influencing gene expression. Researchers have observed expression of this protein mainly in the brain especially in the cerebral cortex of humans and other mammals where it plays an important role in neural development.

Biological function summary

ZNF312/FEZF2 regulates important neuronal differentiation processes. It is part of a transcriptional network that influences cell fate decisions in cortical progenitors. ZNF312/FEZF2 acts as a transcriptional repressor and works in synergy with other transcription factors and co-factors although not a direct part of a stable complex. This regulation supports the proper development of the frontal cortex impacting motor neuron identity and connectivity.

Pathways

ZNF312/FEZF2 fits into pathways such as the Wnt signaling pathway and the Notch signaling cascade which control neurodevelopment and neuronal patterning. ZNF312/FEZF2 interacts with paths involving other proteins like TCF4 and Delta-like ligands coordinating the balance between neural progenitor proliferation and differentiation. This orchestrates the spatial and temporal sequences of cortical neuron generation impacting neuronal network formation.

ZNF312/FEZF2 relates to neurodevelopmental disorders such as autism spectrum disorder and schizophrenia. Altered expression or mutations of FEZF2 can disrupt neural circuit formation affecting mental processes and behavior. FEZF2 ties to other proteins like Disrupted-in-Schizophrenia 1 (DISC1) with evidence that mutations or dysfunctional interactions impact schizophrenia progression suggesting their role in neuronal tissue morphogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription repressor. Required for the specification of corticospinal motor neurons and other subcerebral projection neurons. May play a role in layer and neuronal subtype-specific patterning of subcortical projections and axonal fasciculation. Controls the development of dendritic arborization and spines of large layer V pyramidal neurons. Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation.

See full target information Fezf2

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1277365 PubMed38420512

2024

Ehf and Fezf2 regulate late medullary thymic epithelial cell and thymic tuft cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Sören Lammers,Victor Barrera,Philip Brennecke,Corey Miller,Joon Yoon,Jared Balolong,Mark S Anderson,Shannan Ho Sui,Lars M Steinmetz,Ulrich H von Andrian,Kristin Rattay

eNeuro 8: PubMed33495243

2021

Neuregulin-4 Is Required for Maintaining Soma Size of Pyramidal Neurons in the Motor Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Paramo,Sven O Bachmann,Stéphane J Baudouin,Isabel Martinez-Garay,Alun M Davies

Laboratory investigation; a journal of technical m 98:1225-1236 PubMed29925938

2018

Loss of Fezf2 promotes malignant progression of bladder cancer by regulating the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohui Chen,Lijie Zhou,Xuehan Liu,Longwang Wang,Gallina Kazobinka,Xiaoping Zhang,Teng Hou

The Journal of biological chemistry 291:7661-8 PubMed26861874

2016

Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chunsheng Qu,Dandan Bian,Xue Li,Jian Xiao,Chunping Wu,Yue Li,Tian Jiang,Xiangtian Zhou,Jia Qu,Jie-Guang Chen

Carcinogenesis 34:1984-93 PubMed23677067

2013

FEZF2, a novel 3p14 tumor suppressor gene, represses oncogene EZH2 and MDM2 expression and is frequently methylated in nasopharyngeal carcinoma.

Applications

Species

Unspecified reactive species

Xing-Sheng Shu,Lili Li,Mingfang Ji,Yingduan Cheng,Jianming Ying,Yichao Fan,Lan Zhong,Xiaodong Liu,Sai Wah Tsao,Anthony T C Chan,Qian Tao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF312/FEZF2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (AB69436)

Western blot - Anti-ZNF312/FEZF2 antibody (AB69436)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise