Rabbit Polyclonal ZNF312/FEZF2 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 4 publications.
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- eNeuro 8:2021Neuregulin-4 Is Required for Maintaining Soma Size of Pyramidal Neurons in the Motor Cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBlanca Paramo,Sven O Bachmann,Stéphane J Baudouin,Isabel Martinez-Garay,Alun M DaviesPubMed 33495243
- Laboratory investigation; a journal of technical m 98:1225-12362018Loss of Fezf2 promotes malignant progression of bladder cancer by regulating the NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaohui Chen et. al.PubMed 29925938
- The Journal of biological chemistry 291:7661-82016Transient Expression of Fez Family Zinc Finger 2 Protein Regulates the Brn3b Gene in Developing Retinal Ganglion Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunsheng Qu et. al.PubMed 26861874
- Carcinogenesis 34:1984-932013FEZF2, a novel 3p14 tumor suppressor gene, represses oncogene EZH2 and MDM2 expression and is frequently methylated in nasopharyngeal carcinoma.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human, Rat | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Human, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription repressor. Required for the specification of corticospinal motor neurons and other subcerebral projection neurons. May play a role in layer and neuronal subtype-specific patterning of subcortical projections and axonal fasciculation. Controls the development of dendritic arborization and spines of large layer V pyramidal neurons. Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation.
Alternative names
Fez, Fezl, Zfp312, Fezf2, Fez family zinc finger protein 2, Forebrain embryonic zinc finger-like protein 2, Zinc finger protein 312, Zinc finger protein Fez-like
Recommended products
Rabbit Polyclonal ZNF312/FEZF2 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNF312 also known as FEZF2 (Forebrain Embryonic Zinc Finger-Like Protein 2) is a transcription factor with a mass of approximately 68 kDa. This protein features zinc finger motifs that interact with specific DNA sequences. ZNF312/FEZF2 promotes transcriptional regulation by binding to DNA and influencing gene expression. Researchers have observed expression of this protein mainly in the brain especially in the cerebral cortex of humans and other mammals where it plays an important role in neural development.
- Biological function summary
ZNF312/FEZF2 regulates important neuronal differentiation processes. It is part of a transcriptional network that influences cell fate decisions in cortical progenitors. ZNF312/FEZF2 acts as a transcriptional repressor and works in synergy with other transcription factors and co-factors although not a direct part of a stable complex. This regulation supports the proper development of the frontal cortex impacting motor neuron identity and connectivity.
- Pathways
ZNF312/FEZF2 fits into pathways such as the Wnt signaling pathway and the Notch signaling cascade which control neurodevelopment and neuronal patterning. ZNF312/FEZF2 interacts with paths involving other proteins like TCF4 and Delta-like ligands coordinating the balance between neural progenitor proliferation and differentiation. This orchestrates the spatial and temporal sequences of cortical neuron generation impacting neuronal network formation.
- Associated diseases and disorders
ZNF312/FEZF2 relates to neurodevelopmental disorders such as autism spectrum disorder and schizophrenia. Altered expression or mutations of FEZF2 can disrupt neural circuit formation affecting mental processes and behavior. FEZF2 ties to other proteins like Disrupted-in-Schizophrenia 1 (DISC1) with evidence that mutations or dysfunctional interactions impact schizophrenia progression suggesting their role in neuronal tissue morphogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-ZNF312/FEZF2 antibody (ab69436)
ab69436 staining Fezf2 in Neuro2a cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab69436 at 1/1000 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). The secondary antibody (shown in green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-ZNF312/FEZF2 antibody (ab69436)
All lanes: Western blot - Anti-ZNF312/FEZF2 antibody (ab69436) at 1 µg/mL
All lanes: Spinal Cord (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 17 kDa, 33 kDa, 48 kDa
Exposure time: 8min
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