Rabbit Polyclonal ZNF316 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZNF316 aa 450-550.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ZNF316 aa 450-550. The exact immunogen used to generate this antibody is proprietary information. Database link A6NFI3
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Alternative names
Zinc finger protein 316, ZNF316
Recommended products
Rabbit Polyclonal ZNF316 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ZNF316 aa 450-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ZNF316 aa 450-550. The exact immunogen used to generate this antibody is proprietary information. Database link A6NFI3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab176708 was affinity purified using an epitope specific to ZNF316 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNF316 also known as Zinc Finger Protein 316 acts as a transcription factor involved in regulating gene expression. It possesses multiple C2H2-type zinc fingers that allow it to bind specifically to DNA sequences influencing transcriptional activity. The protein has an approximate mass of 70 kDa based on sequence data analysis. ZNF316 is expressed in various tissues with notable expression in the brain and reproductive organs suggesting its role in diverse physiological processes.
- Biological function summary
Zinc finger proteins like ZNF316 contribute to protein-DNA interactions that regulate gene transcription. This protein may interact with other transcriptional regulators to modulate gene expression patterns essential for cell differentiation and development. While ZNF316 has not been documented as part of a well-characterized multi-protein complex its zinc finger domains suggest a potential for forming interactions with co-regulatory proteins or elements influencing chromatin structure.
- Pathways
ZNF316 has a role in the transcriptional regulation within pathways related to neural development and reproductive function. Specifically it appears involved in pathways with genes that are critical for signaling in neural tissue and may interact functionally within the Wnt signaling cascade. This interaction can link it to proteins such as β-catenin although further research may clarify additional pathway-specific interactions.
- Associated diseases and disorders
Scientific studies suggest a relationship between ZNF316 and neurological conditions such as schizophrenia. Aberrations in the expression or function of ZNF316 may contribute to pathogenesis potentially due to disrupted gene regulatory mechanisms in the brain. Furthermore gastrointestinal cancers have been linked to dysregulation in ZNF316 expression or activity possibly involving its interaction with tumor suppressor proteins like p53 therefore affecting cell proliferation and apoptosis. Continued research is necessary to elucidate these associations in greater detail.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ZNF316 antibody (ab176708)
All lanes: Western blot - Anti-ZNF316 antibody (ab176708) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 30s
Immunoprecipitation - Anti-ZNF316 antibody (ab176708)
ab176708 at 0.4 μg/ml detecting ZNF316 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176708 at 6 μg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of ZNF316.
Lane 3: Control IgG.In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes: Immunoprecipitation - Anti-ZNF316 antibody (ab176708)
Predicted band size: 108 kDa
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