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AB176708

Anti-ZNF316 antibody

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(1 Publication)

Rabbit Polyclonal ZNF316 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZNF316 aa 450-550.

View Alternative Names

Zinc finger protein 316, ZNF316

2 Images
Immunoprecipitation - Anti-ZNF316 antibody (AB176708)
  • IP

Supplier Data

Immunoprecipitation - Anti-ZNF316 antibody (AB176708)

ab176708 at 0.4 μg/ml detecting ZNF316 in HeLa whole cell lysate by WB following IP.

Lane 1 : ab176708 at 6 μg/mg of lysate
Lane 2 : IP with an antibody which recognizes an downstream epitope of ZNF316.
Lane 3 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-ZNF316 antibody (ab176708)

Predicted band size: 108 kDa

false

Western blot - Anti-ZNF316 antibody (AB176708)
  • WB

Supplier Data

Western blot - Anti-ZNF316 antibody (AB176708)

All lanes:

Western blot - Anti-ZNF316 antibody (ab176708) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 108 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ZNF316 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

A6NFI3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab176708 was affinity purified using an epitope specific to ZNF316 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF316 also known as Zinc Finger Protein 316 acts as a transcription factor involved in regulating gene expression. It possesses multiple C2H2-type zinc fingers that allow it to bind specifically to DNA sequences influencing transcriptional activity. The protein has an approximate mass of 70 kDa based on sequence data analysis. ZNF316 is expressed in various tissues with notable expression in the brain and reproductive organs suggesting its role in diverse physiological processes.
Biological function summary

Zinc finger proteins like ZNF316 contribute to protein-DNA interactions that regulate gene transcription. This protein may interact with other transcriptional regulators to modulate gene expression patterns essential for cell differentiation and development. While ZNF316 has not been documented as part of a well-characterized multi-protein complex its zinc finger domains suggest a potential for forming interactions with co-regulatory proteins or elements influencing chromatin structure.

Pathways

ZNF316 has a role in the transcriptional regulation within pathways related to neural development and reproductive function. Specifically it appears involved in pathways with genes that are critical for signaling in neural tissue and may interact functionally within the Wnt signaling cascade. This interaction can link it to proteins such as β-catenin although further research may clarify additional pathway-specific interactions.

Scientific studies suggest a relationship between ZNF316 and neurological conditions such as schizophrenia. Aberrations in the expression or function of ZNF316 may contribute to pathogenesis potentially due to disrupted gene regulatory mechanisms in the brain. Furthermore gastrointestinal cancers have been linked to dysregulation in ZNF316 expression or activity possibly involving its interaction with tumor suppressor proteins like p53 therefore affecting cell proliferation and apoptosis. Continued research is necessary to elucidate these associations in greater detail.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information ZNF316
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40608049

2025

Sphingolipid synthesis maintains nuclear membrane integrity and genome stability during cell division.

Applications

Unspecified application

Species

Unspecified reactive species

Sunyoung Hwang,William Russo,Jaylah Cormier,Jillian Johnson,Sara Martin,Marica Rosaria Ippolito,Sara Cordone,Rui Li,Lihua Julie Zhu,Stefano Santaguida,Eduardo M Torres
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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