AB157459

Anti-ZNF365 antibody [EPR10216]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal ZNF365 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA0844, ZNF365, Talanin

4 Images

Flow Cytometry (Intracellular) - Anti-ZNF365 antibody [EPR10216] (AB157459)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ZNF365 antibody [EPR10216] (AB157459)

Intracellular Intracellular Flow Cytometic analysis of permeabilized Jurkat cells labeling ZNF365 with ab157459 at 1/10 dilution (red) or a rabbit IgG negative (green).

Immunocytochemistry/ Immunofluorescence - Anti-ZNF365 antibody [EPR10216] (AB157459)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZNF365 antibody [EPR10216] (AB157459)

Immunofluorescent analysis of Jurkat cells labeling ZNF365 with ab157459 at 1/50 dilution.

Unknown

Immunoprecipitation - Anti-ZNF365 antibody [EPR10216] (AB157459)

Detection of ZNF365 by Western Blot of Immunprecipitate. Jurkat cell lysate immunoprecipitated using ab157459 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-ZNF365 antibody [EPR10216] (ab157459) at 1/1000 dilution

All lanes:

Immunoprecipitation pellet from Jurkat cell lysate at 10 µg

Predicted band size: 24 kDa

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Lab

Western blot - Anti-ZNF365 antibody [EPR10216] (AB157459)

All lanes:

Western blot - Anti-ZNF365 antibody [EPR10216] (ab157459) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 24 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10216

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The zinc finger protein 365 (ZNF365) also known as ZBRK1 is a transcription factor weighing approximately 107 kDa. This protein plays a mechanical role by binding to specific DNA sequences regulating the transcription of target genes. ZNF365 is expressed in various tissues including the brain heart and skeletal muscle. Its expression pattern suggests it may have versatile functions in different physiological contexts.

Biological function summary

ZNF365 acts as a critical regulatory component in maintaining genomic stability. It associates with the BRCA1 complex which is essential for repairing DNA double-strand breaks. By interacting with this complex ZNF365 participates in controlling cell cycle checkpoints ensuring accurate DNA replication and division. This process helps to prevent the accumulation of genomic abnormalities which could lead to diseases.

Pathways

This protein influences the DNA damage response and cell cycle regulation pathways. ZNF365 works in conjunction with protein partners like BRCA1 and RAD50 facilitating efficient repair of damaged DNA. Its involvement in these pathways ensures cells can respond to genetic insults timely and effectively safeguarding genetic information and controlling cellular proliferation rates.

ZNF365 has a notable connection with cancer risk particularly breast cancer. Alterations in its function or expression can disrupt genome stability contributing to tumor genesis and progression. ZNF365 also interacts with p53 a tumor suppressor protein. Defects in these interactions may further compromise DNA repair processes highlighting its significance in cancer biology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in uric acid excretion.

See full target information ZNF365

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Autophagy reports 4:2476218 PubMed40458442

2025

The TECPR1:ATG5-ATG12 complex conjugates LC3/ATG8 to damaged lysosomes that expose luminal glycans in response to osmotic imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxue Wang,Matthew Jefferson,Maria Ramos,Matthew Whelband,Kristin Kreuzer,Grace Khuu,Michael Lazarou,James Mccoll,James Lazenby,Cynthia B Whitchurch,Paul Verkade,Ulrike Mayer,Thomas Wileman

Cells 11: PubMed36139424

2022

Lack of ZNF365 Drives Senescence and Exacerbates Experimental Lung Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Urista,Mariel Maldonado,Fernanda Toscano-Marquez,Remedios Ramírez,Yalbi Itzel Balderas-Martínez,Carina Becerril,Yair Romero,Moisés Selman,Annie Pardo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF365 antibody [EPR10216]

Flow Cytometry (Intracellular) - Anti-ZNF365 antibody [EPR10216] (AB157459)

Immunocytochemistry/ Immunofluorescence - Anti-ZNF365 antibody [EPR10216] (AB157459)

Immunoprecipitation - Anti-ZNF365 antibody [EPR10216] (AB157459)

Western blot - Anti-ZNF365 antibody [EPR10216] (AB157459)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise