AB176689

Anti-ZNF384 antibody

Name: Anti-ZNF384 antibody (ab176689) | Abcam
Brand: Abcam Limited
SKU: AB176689
Price: 525 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal ZNF384 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ZNF384 aa 500 to C-terminus.

View Alternative Names

CAGH1, CIZ, NMP4, TNRC1, ZNF384, Zinc finger protein 384, CAG repeat protein 1, CAS-interacting zinc finger protein, Nuclear matrix transcription factor 4, Trinucleotide repeat-containing gene 1 protein, Nuclear matrix protein 4

2 Images

Supplier Data

Immunoprecipitation - Anti-ZNF384 antibody (AB176689)

Detection of Human ZNF384 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab176689 used for IP at 6 μg/mg lysate. For blotting immunoprecipitated ZNF384, ab176689 was used at 0.4 μg/ml.

All lanes:

Immunoprecipitation - Anti-ZNF384 antibody (ab176689)

Predicted band size: 63 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-ZNF384 antibody (AB176689)

All lanes:

Western blot - Anti-ZNF384 antibody (ab176689) at 0.04 µg/mL

Lane 1:

HeLa lysate at 50 µg

Lane 2:

HeLa lysate at 15 µg

Lane 3:

293T lysate at 50 µg

Lane 4:

Jurkat lysate at 50 µg

Predicted band size: 63 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ZNF384 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TF68

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF384 also known as zinc finger protein 384 acts as a transcription factor. It has a molecular mass of approximately 55 kDa. This protein contains multiple zinc finger motifs allowing it to bind to DNA. ZNF384 regulates the expression of various genes linked to cellular processes. It localizes to the nucleus where it performs its function. Although broadly expressed the protein shows higher expression in tissues such as the brain and bone marrow.

Biological function summary

This transcription factor plays a role in controlling gene expression networks essential to differentiation and development. ZNF384 contributes significantly to the regulation of genes involved in cell growth and proliferation. It is known to interact with the transcriptional complexes influencing downstream gene expression events that contribute to developmental processes. ZNF384's functionality is important in maintaining the balance of cellular proliferation and differentiation.

Pathways

ZNF384 finds integration within the MAPK signaling pathway and the Wnt signaling pathway. These pathways respond to extracellular signals and control various cellular processes like growth and differentiation. ZNF384 interacts with proteins such as CBP and p300 which are coactivators that modulate transcriptional activities. These interactions help integrate signals from different pathways influencing specific cellular responses.

Researchers associate ZNF384 with leukemia and osteosarcoma. In particular its fusion with transcriptional co-activators like EWS has implications in leukemogenesis often found in mixed-lineage leukemia (MLL). The presence of ZNF384 abnormalities may influence disease progression and patient prognosis. Understanding how ZNF384 interplays with related factors such as TET1 could provide insights into the pathogenesis of these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcription factor that binds the consensus DNA sequence [GC]AAAAA. Seems to bind and regulate the promoters of MMP1, MMP3, MMP7 and COL1A1 (By similarity).

See full target information ZNF384

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70037 PubMed40717692

2025

Cancer-associated fibroblasts-secreted lactate promotes RNA polymerase III subunit G-mediated epithelial-mesenchymal transition in non-small cell lung cancer by increasing m6A modification of zinc finger protein 384.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Xing Yang,Hao Tang,Zhiping Zhou,Bin Liu

Cell death & disease 16:100 PubMed39955289

2025

Nucleolar NOL9 regulated by DNA methylation promotes hepatocellular carcinoma growth through activation of Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiyao Chen,Xin Song,Xingrong Zheng,Tinglin Qian,Boxiang Zhang,Lina Wu,Qinghai Lian,Jia Chen,Qiumin Luo,Wenxiong Xu,Liang Peng,Chan Xie

Cell biology and toxicology 40:100 PubMed39562372

2024

The pivotal role of ZNF384: driving the malignant behavior of serous ovarian cancer cells via the LIN28B/UBD axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yang,Runze He,Dongxiao Li,Tianli Mu,Ziteng Kuang,Min Wang

Cell communication and signaling : CCS 22:211 PubMed38566191

2024

EP300-ZNF384 transactivates IL3RA to promote the progression of B-cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijie Hou,Yifei Ren,Xuehong Zhang,Dan Huang,Fanzhi Yan,Wentao Sun,Wenjuan Zhang,Qingqing Zhang,Xihui Fu,Zhenghui Lang,Chenyang Chu,Boyang Zou,Beibei Gao,Bilian Jin,Zhijie Kang,Quentin Liu,Jinsong Yan

Genes & diseases 10:2622-2638 PubMed37554218

2023

PRMT1 promotes the proliferation and metastasis of gastric cancer cells by recruiting MLXIP for the transcriptional activation of the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Shitong Chen,Shihan Peng,Xujun Zhou,Houyi Tang,Hanghua Liang,Xi Zhong,He Yang,Xiaoxue Ke,MuHan Lü,Hongjuan Cui

Blood cancer discovery 3:240-263 PubMed35247902

2022

ZNF384 Fusion Oncoproteins Drive Lineage Aberrancy in Acute Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten M Dickerson,Chunxu Qu,Qingsong Gao,Ilaria Iacobucci,Zhaohui Gu,Hiroki Yoshihara,Emily A Backhaus,Yunchao Chang,Laura J Janke,Beisi Xu,Gang Wu,Evangelia K Papachristou,Clive S D'Santos,Kathryn G Roberts,Charles G Mullighan

Theranostics 11:4599-4615 PubMed33754016

2021

The histone acetyltransferase HBO1 functions as a novel oncogenic gene in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Yang Gao,Zhuo-Yan Ling,Yun-Rong Zhu,Ce Shi,Yin Wang,Xiang-Yang Zhang,Zhi-Qing Zhang,Qin Jiang,Min-Bin Chen,Shuofei Yang,Cong Cao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF384 antibody

Immunoprecipitation - Anti-ZNF384 antibody (AB176689)

Western blot - Anti-ZNF384 antibody (AB176689)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise