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AB235773

Anti-ZNF384 antibody

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(1 Publication)

Rabbit Polyclonal ZNF384 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF384 aa 1-250.

View Alternative Names

CAGH1, CIZ, NMP4, TNRC1, ZNF384, Zinc finger protein 384, CAG repeat protein 1, CAS-interacting zinc finger protein, Nuclear matrix transcription factor 4, Trinucleotide repeat-containing gene 1 protein, Nuclear matrix protein 4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF384 antibody (AB235773)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF384 antibody (AB235773)

Paraffin-embedded human glioma tissue stained for ZNF384 with ab235773 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF384 antibody (AB235773)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF384 antibody (AB235773)

Paraffin-embedded human melanoma tissue stained for ZNF384 with ab235773 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ZNF384 antibody (AB235773)
  • WB

Supplier Data

Western blot - Anti-ZNF384 antibody (AB235773)

All lanes:

Western blot - Anti-ZNF384 antibody (ab235773) at 1/1000 dilution

Lane 1:

EC109 whole cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/15000 dilution

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZNF384 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q8TF68

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF384 also known as zinc finger protein 384 acts as a transcription factor. It has a molecular mass of approximately 55 kDa. This protein contains multiple zinc finger motifs allowing it to bind to DNA. ZNF384 regulates the expression of various genes linked to cellular processes. It localizes to the nucleus where it performs its function. Although broadly expressed the protein shows higher expression in tissues such as the brain and bone marrow.
Biological function summary

This transcription factor plays a role in controlling gene expression networks essential to differentiation and development. ZNF384 contributes significantly to the regulation of genes involved in cell growth and proliferation. It is known to interact with the transcriptional complexes influencing downstream gene expression events that contribute to developmental processes. ZNF384's functionality is important in maintaining the balance of cellular proliferation and differentiation.

Pathways

ZNF384 finds integration within the MAPK signaling pathway and the Wnt signaling pathway. These pathways respond to extracellular signals and control various cellular processes like growth and differentiation. ZNF384 interacts with proteins such as CBP and p300 which are coactivators that modulate transcriptional activities. These interactions help integrate signals from different pathways influencing specific cellular responses.

Researchers associate ZNF384 with leukemia and osteosarcoma. In particular its fusion with transcriptional co-activators like EWS has implications in leukemogenesis often found in mixed-lineage leukemia (MLL). The presence of ZNF384 abnormalities may influence disease progression and patient prognosis. Understanding how ZNF384 interplays with related factors such as TET1 could provide insights into the pathogenesis of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds the consensus DNA sequence [GC]AAAAA. Seems to bind and regulate the promoters of MMP1, MMP3, MMP7 and COL1A1 (By similarity).
See full target information ZNF384
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 11:9778-9793 PubMed31727867

2019

Ubiquitin specific peptidase 5 promotes ovarian cancer cell proliferation through deubiquitinating HDAC2.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhua Du,Jun Lin,Rulin Zhang,Wanli Yang,Heng Quan,Lijuan Zang,Yaqin Han,Bing Li,Hong Sun,Jun Wu
View all publications
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Product promise

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