AB241962

Anti-ZNF451/COASTER antibody

Name: Anti-ZNF451/COASTER antibody (ab241962) | Abcam
Brand: Abcam Limited
SKU: AB241962
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ZNF451/COASTER antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZNF451 aa 1000 to C-terminus.

View Alternative Names

COASTER, KIAA0576, KIAA1702, ZNF451, E3 SUMO-protein ligase ZNF451, Coactivator for steroid receptors, E3 SUMO-protein transferase ZNF451, Zinc finger protein 451

2 Images

Supplier Data

Immunoprecipitation - Anti-ZNF451/COASTER antibody (AB241962)

ZNF451/COASTER was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241962 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241962 at 1 μg/ml.

Lane 1 : ab241962 IP in Jurkat whole cell lysate.
Lane 2 : Control IgG IP in Jurkat whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-ZNF451/COASTER antibody (ab241962)

Predicted band size: 121 kDa

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Supplier Data

Western blot - Anti-ZNF451/COASTER antibody (AB241962)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-ZNF451/COASTER antibody (ab241962) at 0.2 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 121 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ZNF451 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4E5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to ZNF451/COASTER immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF451 also known as COASTER is a protein with a molecular mass of approximately 148 kDa. It functions as a transcription factor by binding to specific DNA sequences regulating the expression of certain genes. ZNF451 achieves this through its zinc finger motifs known for DNA interaction. It contains N-terminal PPP1 binding domain which plays a role in its molecular function. Expression of ZNF451 is noted in various tissues with a higher presence in the heart and skeletal muscle indicating its role in these areas.

Biological function summary

ZNF451 participates in chromatin remodeling an important process in regulating gene expression. It is part of a larger complex that includes components like histone deacetylase. This complex influences chromatin structure by deacetylating histones facilitating transcriptional repression. Through these actions ZNF451 modulates cell cycle regulation and developmental processes. Its involvement in such foundational cellular functions highlights its importance in cell differentiation and growth.

Pathways

ZNF451 plays a significant role in the TGF-beta signaling pathway and the Hippo pathway. In the TGF-beta pathway ZNF451 interacts with SMAD proteins which are critical for transmitting extracellular signals into the cell nucleus affecting gene expression. This interaction modulates processes such as cell proliferation and differentiation. In the Hippo pathway ZNF451 relates to YAP/TAZ proteins helping regulate organ size by controlling cell proliferation and apoptosis. Both pathways underline ZNF451's involvement in maintaining cellular homeostasis and growth.

ZNF451 has connections to cardiovascular diseases and certain muscle disorders. It has been implicated in dilated cardiomyopathy where its dysregulation can affect the structural integrity and function of cardiac muscle. ZNF451’s interaction with cardiac-specific proteins can contribute to the remodeling seen in these conditions. In muscle disorders it influences muscle differentiation and regeneration suggesting that altered ZNF451 function could impact muscle repair. These associations indicate ZNF451's potential as a target for therapeutic interventions in related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins (PubMed : 26524493, PubMed : 26524494). Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation (By similarity). Has no activity with SUMO1 (PubMed : 26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11' (PubMed : 26524493). Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9' (PubMed : 24324267). Plays a role in regulating the transcription of AR targets (PubMed : 18656483).

See full target information ZNF451

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed33079728

2020

Guanosine triphosphate links MYC-dependent metabolic and ribosome programs in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Huang,Kenneth E Huffman,Zixi Wang,Xun Wang,Kailong Li,Feng Cai,Chendong Yang,Ling Cai,Terry S Shih,Lauren G Zacharias,Andrew Chung,Qian Yang,Milind D Chalishazar,Abbie S Ireland,C Allison Stewart,Kasey Cargill,Luc Girard,Yi Liu,Min Ni,Jian Xu,Xudong Wu,Hao Zhu,Benjamin Drapkin,Lauren A Byers,Trudy G Oliver,Adi F Gazdar,John D Minna,Ralph J DeBerardinis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF451/COASTER antibody

Immunoprecipitation - Anti-ZNF451/COASTER antibody (AB241962)

Western blot - Anti-ZNF451/COASTER antibody (AB241962)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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