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AB204974

Anti-ZNF471/ERP1 antibody

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(3 Publications)

Rabbit Polyclonal ZNF471/ERP1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF471 aa 50 to C-terminus.

View Alternative Names

ERP1, KIAA1396, ZNF471, Zinc finger protein 471, EZFIT-related protein 1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling ZNF471/ERP1 with ab204974 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol. No positivity as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling ZNF471/ERP1 with ab204974 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling ZNF471/ERP1 with ab204974 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-ZNF471/ERP1 antibody (AB204974)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZNF471/ERP1 antibody (AB204974)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling ZNF471/ERP1 in the nucleus with ab204974 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF471/ERP1 antibody (AB204974)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ZNF471/ERP1 in the cytoplasm and nucleus of glandular cells with ab204974 at a 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-ZNF471/ERP1 antibody (AB204974)
  • WB

Supplier Data

Western blot - Anti-ZNF471/ERP1 antibody (AB204974)

All lanes:

Western blot - Anti-ZNF471/ERP1 antibody (ab204974) at 0.4 µg/mL

All lanes:

U-2 OS cells

Predicted band size: 73 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZNF471 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BX82

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF471 also known as ERP1 is a zinc finger protein that plays a significant mechanical role in cellular processes. It has an approximate molecular weight of around 75 kDa. The protein is expressed in various tissues with a higher presence observed in epithelial tissues. As a zinc finger protein ZNF471 binds to DNA influencing transcriptional regulation. ZNF471/ERP1 is important in maintaining the integrity of gene expression by interacting with specific DNA sequences.
Biological function summary

This zinc finger protein regulates transcription factors and affects gene expression patterns. ZNF471/ERP1 does not exist as part of a larger protein complex allowing it to function more independently. Through its ability to bind DNA it influences the transcriptional activity of multiple key genes involved in cellular growth differentiation and development. It has a significant role in signaling pathways linked to cellular proliferation.

Pathways

ZNF471/ERP1 interacts with transcriptional machinery to regulate the Wnt/β-catenin and TP53 pathways. Within these pathways it plays a regulator role modulating gene expressions necessary for cellular growth and apoptosis. For instance it acts in concert with proteins such as β-catenin and TP53 to either promote or inhibit the transcription of genes depending on the cellular context. These regulatory activities highlight its importance in maintaining cellular homeostasis.

Aberrations in ZNF471/ERP1 expression have associations with cancer specifically colorectal and breast cancer. Dysregulation can lead to uncontrolled cellular proliferation and resistance to apoptosis which are hallmarks of cancer initiation and progression. The protein's interaction with other pivotal proteins such as TP53 and β-catenin in these cancers indicates its therapeutic and diagnostic potential in oncology as researchers explore targeted treatments that may influence this interaction pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information ZNF471
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical epigenetics 12:173 PubMed33203470

2020

The tumor suppressor Zinc finger protein 471 suppresses breast cancer growth and metastasis through inhibiting AKT and Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfang Tao,Juan Luo,Jun Tang,Danfeng Zhou,Shujun Feng,Zhu Qiu,Thomas C Putti,Tingxiu Xiang,Qiao Tao,Lili Li,Guosheng Ren

Cancer cell international 20:180 PubMed32489316

2020

lncRNA LIFR-AS1 suppresses invasion and metastasis of non-small cell lung cancer via the miR-942-5p/ZNF471 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Wang,Jing Wu,Hui Huang,Yan Jiang,Ying Huang,Hongyan Fang,Gang Zheng,Xiaochun Zhou,Yujuan Wu,Changjiang Lei,Desheng Hu

Molecular cancer research : MCR 14:841-8 PubMed27280713

2016

Downregulation of the Deiminase PADI2 Is an Early Event in Colorectal Carcinogenesis and Indicates Poor Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Neus Cantariño,Eva Musulén,Vanesa Valero,Miquel Angel Peinado,Manuel Perucho,Victor Moreno,Sònia-Vanina Forcales,Julien Douet,Marcus Buschbeck
View all publications
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Product promise

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