AB250223

Anti-ZNF529 antibody [EPR13674] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ZNF529 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA1615, ZNF529, Zinc finger protein 529

1 Images

Supplier Data

Western blot - Anti-ZNF529 antibody [EPR13674] - BSA and Azide free (AB250223)

This data was developed using ab180526, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNF529 antibody [EPR13674] (<a href='/en-us/products/primary-antibodies/znf529-antibody-epr13674-ab180526'>ab180526</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

MCF-7 cell lysate at 20 µg

Lane 4:

MDA-MB-435 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 66 kDa

false

Unconjugated

Anti-ZNF529 antibody [EPR13674]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13674

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab250223 is the carrier-free version of ab180526.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF529 also known as Zinc Finger Protein 529 functions as a transcription factor. Its primary role involves binding to specific DNA sequences influencing gene expression. ZNF529 has an approximate molecular weight of 79 kDa. Expression of ZNF529 occurs in several tissues with notable presence in the brain and reproductive organs. Its zinc finger motifs enable it to interact with diverse genetic elements affecting the transcriptional landscape.

Biological function summary

ZNF529 participates in regulating developmental processes and differentiation pathways. The protein acts as part of a transcriptional control complex interacting with other regulatory proteins to modulate specific gene sets. By influencing the expression of genes involved in cell growth and survival ZNF529 plays a critical role in maintaining cellular functions. Researchers have linked these interactions to processes such as neuronal development and tissue-specific gene expression.

Pathways

ZNF529 contributes to key pathways like the Wnt signaling pathway and the Notch signaling pathway. Within these pathways it interacts with significant proteins such as beta-catenin in Wnt signaling and the Notch intracellular domain in Notch signaling. These pathways are essential for processes such as embryonic development and stem cell maintenance. ZNF529 aids in the regulation and balance of these pathways highlighting its role in cellular communication and signaling networks.

Abnormal ZNF529 expression associates with neurological disorders and certain cancers. Dysregulation of ZNF529 may lead to conditions such as schizophrenia where its interplay with proteins like DISC1 has been observed. Additionally ZNF529 may contribute to tumorigenesis through interaction with cancer-associated proteins such as p53. Understanding ZNF529's involvement in these diseases could provide insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in transcriptional regulation.

See full target information ZNF529

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNF529 antibody [EPR13674] - BSA and Azide free

Western blot - Anti-ZNF529 antibody [EPR13674] - BSA and Azide free (AB250223)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise