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AB279394

Anti-ZNF545 antibody [OTI5D8]

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Mouse Monoclonal ZNF545 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ZFP82.

View Alternative Names

KIAA1948, ZNF545, ZFP82, Zinc finger protein 82 homolog, Zfp-82, Zinc finger protein 545

1 Images
Western blot - Anti-ZNF545 antibody [OTI5D8] (AB279394)
  • WB

Supplier Data

Western blot - Anti-ZNF545 antibody [OTI5D8] (AB279394)

All lanes:

Western blot - Anti-ZNF545 antibody [OTI5D8] (ab279394) at 1/500 dilution

All lanes:

HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts at 35 µg

Predicted band size: 63 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI5D8

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ZFP82.

Q8N141

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF545 also known as Zinc Finger Protein 545 is a transcriptional repressor that plays a role in gene expression regulation. The protein has a mass of approximately 77 kDa and contains typical C2H2 zinc finger domains that facilitate DNA binding. ZNF545 expresses mainly in the nucleus of cells within several tissues including those in the digestive and reproductive systems. It acts by binding to specific DNA sequences blocking the access of transcriptional machinery and downregulating the transcription of target genes.
Biological function summary

ZNF545 involves itself in cellular growth and proliferation processes. It functions as part of a repressive complex that influences the expression of tumor suppressor genes. The complex can modify chromatin structure by recruiting histone deacetylases which contribute to gene silencing. ZNF545's activity helps balance cell cycle progression and prevent unwanted cellular growth making it a candidate for suppressing tumorigenicity within epithelial cells.

Pathways

The protein interacts notably in pathways involved in cell cycle regulation and apoptosis. ZNF545 has a role within the p53 signaling pathway a critical pathway for maintaining cellular integrity and response to DNA damage. It potentially regulates the expression of genes within this pathway by interacting with other proteins like MDM2 which in turn modulate p53 levels and activity. This interaction ensures accurate response to cellular stress or damage preventing proliferation of damaged cells.

ZNF545 has connections to various cancers particularly gastric and nasopharyngeal carcinomas. In many cases the ZNF545 gene undergoes epigenetic silencing which leads to reduced expression and loss of its tumor-suppressing function. Methylation of its promoter region associates with the dysregulation commonly observed in these cancers. ZNF545's interaction with p53 and its impact on tumorigenic pathways highlights its importance as a therapeutic target and biomarker in oncology research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information ZFP82
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