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AB236428

Anti-ZNF558 antibody [OTI2C4]

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(1 Publication)

Mouse Monoclonal ZNF558 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Zinc finger protein 558.

View Alternative Names

Zinc finger protein 558, ZNF558

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF558 antibody [OTI2C4] (AB236428)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF558 antibody [OTI2C4] (AB236428)

Paraffin-embedded human kidney tissue stained for ZNF558 using ab236428 at 1/150 dilution in immunohistochemical analysis.

Western blot - Anti-ZNF558 antibody [OTI2C4] (AB236428)
  • WB

Supplier Data

Western blot - Anti-ZNF558 antibody [OTI2C4] (AB236428)

All lanes:

Western blot - Anti-ZNF558 antibody [OTI2C4] (ab236428) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY ZNF558 cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Predicted band size: 46 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2C4

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Zinc finger protein 558.

Q96NG5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer, pH 8.5 at 120°C for 3 minutes.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF558 also known as Zinc Finger Protein 558 functions as a transcription factor with a mass of approximately 88 kDa. It contains a series of zinc finger motifs that enable it to bind DNA thereby regulating gene expression. ZNF558 sees expression in various tissues including the brain and immune system. Through its action it influences transcriptional activity and affects downstream gene regulation which can modify cellular processes.
Biological function summary

The role of ZNF558 involves controlling the expression of genes related to cellular growth and differentiation. It acts dynamically within specific gene-regulatory networks rather than being part of a stable protein complex. The biological impact of ZNF558 on cellular processes indicates its importance in maintaining cellular homeostasis. Its function in developmental pathways suggests that it interacts with other zinc finger proteins to fine-tune gene expression responses to environmental and cellular conditions.

Pathways

ZNF558 participates in the transcription regulatory network. It plays a part in the Wnt signaling pathway influencing important cellular responses such as proliferation and differentiation. Through this pathway ZNF558 interacts with other proteins like β-catenin which furthers the signal transduction to drive transcriptional outcomes. Additionally the protein links with the Notch signaling pathway which can affect gene expression diversity.

Aberrant expression or mutations of ZNF558 associate with certain cancers and neurological disorders. In some cancer types altered levels of ZNF558 can affect cell cycle control by interacting with tumor suppressor proteins leading to uncontrollable cell proliferation. On the neurological front its irregular function potentially contributes to disorders involving neurodevelopment alongside proteins like REST which regulate neuronal gene expression and are significant for proper neuronal functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information Zinc finger protein 558
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:489 PubMed40133702

2025

PTOV1 interacts with ZNF449 to promote colorectal cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Wang,Xinwei Qiao,Kaming Xue,Qianzhi Chen,Anshu Li
View all publications
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Product promise

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