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AB85933

Anti-ZNF574 antibody

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(1 Publication)

Rabbit Polyclonal ZNF574 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Zinc finger protein 574.

View Alternative Names

Zinc finger protein 574, ZNF574

2 Images
Immunoprecipitation - Anti-ZNF574 antibody (AB85933)
  • IP

Unknown

Immunoprecipitation - Anti-ZNF574 antibody (AB85933)

Detection of human ZNF54 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85933 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZNF574 antibody (ab85933)

Predicted band size: 99 kDa

false

Western blot - Anti-ZNF574 antibody (AB85933)
  • WB

Unknown

Western blot - Anti-ZNF574 antibody (AB85933)

developed using the ECL technique

All lanes:

Western blot - Anti-ZNF574 antibody (ab85933) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 99 kDa

Observed band size: 120 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human Zinc finger protein 574. The exact immunogen used to generate this antibody is proprietary information.

Q6ZN55

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab85933 was affinity purified using an epitope specific to ZNF574 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF574 also known as Zinc Finger Protein 574 functions as a transcription factor. This protein contains zinc finger domains that allow DNA binding which modulates the expression of specific genes. It has a molecular mass of approximately 74 kDa. Human cells mainly express ZNF574 in tissues such as the liver brain and kidneys where it acts within the nucleus to influence gene transcription.
Biological function summary

Zinc Finger Protein 574 regulates gene expression by binding to DNA at specific sites. As a transcription factor it plays a role in controlling developmental and cellular processes. ZNF574 may function as part of a multiprotein complex collaborating with other transcription factors to enhance or suppress gene transcription. By controlling specific genes it contributes to cell differentiation and the maintenance of normal cellular functions.

Pathways

Zinc Finger Protein 574 participates in essential gene regulatory pathways. It influences cellular processes like cell cycle regulation and apoptosis. Through these pathways ZNF574 interacts with proteins such as p53 a known tumor suppressor and components of the Wnt signaling pathway affecting diverse biological activities that maintain cellular health and contribute to organismal development.

Aberrations in ZNF574 have connections to cancers and neurological disorders. Altered expression levels or mutations can disrupt normal cellular functions leading to tumorigenesis or neurodegenerative conditions. Its relationship with proteins like p53 links it further to cancer biology where imbalance in its regulatory functions may promote malignant cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of a ribosome quality control that mediates surveillance of ribosome assembly and promotes degradation of ribosomes with assembly defects.
See full target information Zinc finger protein 574
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 32:3618-3628 PubMed39066480

2024

CRISPR-mediated ablation of TP53 and EGFR mutations enhances gefitinib sensitivity and anti-tumor efficacy in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

A-Rum Yoon,Soyeon Lee,Ju Hee Kim,Yejin Park,Taeyoung Koo,Chae-Ok Yun
View all publications
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Product promise

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