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Rabbit Recombinant Monoclonal ZNF575 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-ZNF575 antibody [EPR9027] (AB138499), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] (AB138499), expandable thumbnail
  • OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] (AB138499), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Predicted
Not recommended
Not recommended
Predicted
Rat
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

May be involved in transcriptional regulation.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ZNF575 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9027
Purity
Tissue culture supernatant
Dissociation constant
1.35 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ZNF575 protein also known as Zinc Finger Protein 575 weighs approximately 65 kDa. It contains zinc finger motifs that facilitate DNA binding. These motifs are involved in the regulation of gene transcription. Researchers have detected ZNF575 expression mainly in the brain and reproductive tissues indicating its function may concentrate in these regions.

Biological function summary

ZNF575 participates in the regulation of gene expression. It acts as a transcription factor directly influencing the activity of various genes. Although not consistently reported as being part of large molecular complexes ZNF575 can interact with other transcription factors and cofactors. This interaction suggests a role in modulating developmental and cell differentiation processes.

Pathways

ZNF575 interacts with key pathways like the Wnt signaling and p53 pathway. The Wnt signaling pathway controls critical aspects of cell fate including cellular migration and organogenesis. Its role in the p53 pathway links ZNF575 to the regulation of cell cycle and apoptosis through associations with other proteins like beta-catenin and various p53-regulated genes.

Associated diseases and disorders

The presence of ZNF575 has implications for neurodegenerative diseases and certain cancers. Misregulation of ZNF575 can influence neuronal function potentially contributing to conditions like Alzheimer's disease. In cancer altered expression of ZNF575 can be associated with tumorigenesis through its interaction with the p53 protein affecting cell growth and survival.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-ZNF575 antibody [EPR9027] (ab138499), expandable thumbnail

    Western blot - Anti-ZNF575 antibody [EPR9027] (ab138499)

    All lanes: Western blot - Anti-ZNF575 antibody [EPR9027] (ab138499) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: MCF7 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 27 kDa

    Observed band size: 27 kDa

  • Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] (ab138499), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] (ab138499)

    Intracellular flow cytometrical analysis of permeabilized HeLa cells with ab138499 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

  • OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] (ab138499), expandable thumbnail

    OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] (ab138499)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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