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AB248850

Anti-ZNF575 antibody [EPR9027] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ZNF575 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Zinc finger protein 575, ZNF575

3 Images
Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)

This data was developed using ab138499, the same antibody clone in a different buffer formulation.

Intracellular flow cytometrical analysis of permeabilized HeLa cells with ab138499 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

Western blot - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)
  • WB

Unknown

Western blot - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)

This data was developed using ab138499, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNF575 antibody [EPR9027] (<a href='/en-us/products/primary-antibodies/znf575-antibody-epr9027-ab138499'>ab138499</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9027

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab248850 is the carrier-free version of ab138499.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ZNF575 protein also known as Zinc Finger Protein 575 weighs approximately 65 kDa. It contains zinc finger motifs that facilitate DNA binding. These motifs are involved in the regulation of gene transcription. Researchers have detected ZNF575 expression mainly in the brain and reproductive tissues indicating its function may concentrate in these regions.
Biological function summary

ZNF575 participates in the regulation of gene expression. It acts as a transcription factor directly influencing the activity of various genes. Although not consistently reported as being part of large molecular complexes ZNF575 can interact with other transcription factors and cofactors. This interaction suggests a role in modulating developmental and cell differentiation processes.

Pathways

ZNF575 interacts with key pathways like the Wnt signaling and p53 pathway. The Wnt signaling pathway controls critical aspects of cell fate including cellular migration and organogenesis. Its role in the p53 pathway links ZNF575 to the regulation of cell cycle and apoptosis through associations with other proteins like beta-catenin and various p53-regulated genes.

The presence of ZNF575 has implications for neurodegenerative diseases and certain cancers. Misregulation of ZNF575 can influence neuronal function potentially contributing to conditions like Alzheimer's disease. In cancer altered expression of ZNF575 can be associated with tumorigenesis through its interaction with the p53 protein affecting cell growth and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information ZNF575
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com