Rabbit Recombinant Monoclonal ZNF575 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850), expandable thumbnail](https://content.abcam.com/products/images/znf575-antibody-epr9027-bsa-and-azide-free-ab248850--western-blot-img106790.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)")
![Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850), expandable thumbnail](https://content.abcam.com/products/images/znf575-antibody-epr9027-bsa-and-azide-free-ab248850--flow-cytometry-intracellular-img26548.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)")
![OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850), expandable thumbnail](https://content.abcam.com/products/images/znf575-antibody-epr9027-bsa-and-azide-free-ab248850--oi-rd-scanning-img137083.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (AB248850)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Alternative names
Zinc finger protein 575, ZNF575
Recommended products
Rabbit Recombinant Monoclonal ZNF575 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9027
- Purification technique
- Affinity purification
- Dissociation constant
- 1.35 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248850 is the carrier-free version of Anti-ZNF575 antibody [EPR9027] ab138499.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ZNF575 protein also known as Zinc Finger Protein 575 weighs approximately 65 kDa. It contains zinc finger motifs that facilitate DNA binding. These motifs are involved in the regulation of gene transcription. Researchers have detected ZNF575 expression mainly in the brain and reproductive tissues indicating its function may concentrate in these regions.
- Biological function summary
ZNF575 participates in the regulation of gene expression. It acts as a transcription factor directly influencing the activity of various genes. Although not consistently reported as being part of large molecular complexes ZNF575 can interact with other transcription factors and cofactors. This interaction suggests a role in modulating developmental and cell differentiation processes.
- Pathways
ZNF575 interacts with key pathways like the Wnt signaling and p53 pathway. The Wnt signaling pathway controls critical aspects of cell fate including cellular migration and organogenesis. Its role in the p53 pathway links ZNF575 to the regulation of cell cycle and apoptosis through associations with other proteins like beta-catenin and various p53-regulated genes.
- Associated diseases and disorders
The presence of ZNF575 has implications for neurodegenerative diseases and certain cancers. Misregulation of ZNF575 can influence neuronal function potentially contributing to conditions like Alzheimer's disease. In cancer altered expression of ZNF575 can be associated with tumorigenesis through its interaction with the p53 protein affecting cell growth and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850), expandable thumbnail](https://content.abcam.com/products/images/znf575-antibody-epr9027-bsa-and-azide-free-ab248850--western-blot-img106790.jpg "Western blot - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850)")
Western blot - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850)
This data was developed using Anti-ZNF575 antibody [EPR9027] ab138499, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ZNF575 antibody [EPR9027] (Anti-ZNF575 antibody [EPR9027] ab138499) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: MCF7 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
![Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850), expandable thumbnail](https://content.abcam.com/products/images/znf575-antibody-epr9027-bsa-and-azide-free-ab248850--flow-cytometry-intracellular-img26548.jpg "Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850)")
Flow Cytometry (Intracellular) - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850)
This data was developed using Anti-ZNF575 antibody [EPR9027] ab138499, the same antibody clone in a different buffer formulation.
Intracellular flow cytometrical analysis of permeabilized HeLa cells with Anti-ZNF575 antibody [EPR9027] ab138499 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
![OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850), expandable thumbnail](https://content.abcam.com/products/images/znf575-antibody-epr9027-bsa-and-azide-free-ab248850--oi-rd-scanning-img137083.jpg "OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850)")
OI-RD Scanning - Anti-ZNF575 antibody [EPR9027] - BSA and Azide free (ab248850)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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