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AB135921

Anti-ZNF598 antibody

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(2 Publications)

Rabbit Polyclonal ZNF598 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ZNF598.

View Alternative Names

E3 ubiquitin-protein ligase ZNF598, Zinc finger protein 598, ZNF598

1 Images
Western blot - Anti-ZNF598 antibody (AB135921)
  • WB

Unknown

Western blot - Anti-ZNF598 antibody (AB135921)

All lanes:

Western blot - Anti-ZNF598 antibody (ab135921) at 1/500 dilution

All lanes:

Human lung extract at 30 µg

Predicted band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ZNF598. The exact immunogen used to generate this antibody is proprietary information.

Q86UK7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF598 also known as Zinc Finger Protein 598 functions as an E3 ubiquitin ligase involved in ribosome-associated quality control. It has a molecular mass of approximately 98 kDa. This protein contains multiple zinc finger domains that mediate its binding to substrates. ZNF598 localizes predominantly to the cytoplasm specifically associating with ribosomes. Expression occurs in various tissues with higher levels observed in actively dividing cells.
Biological function summary

ZNF598 regulates translation quality control by recognizing and ubiquitinating stalled 80S ribosomal complexes marking them for degradation. This process is part of the Ribosome-Associated Quality Control (RQC) complex. It ensures that defective mRNAs and nascent peptide chains do not accumulate maintaining the fidelity of protein synthesis. Through interaction with other components of the RQC complex ZNF598 prevents the persistence of translation errors that could be harmful to the cell.

Pathways

ZNF598 plays a critical role in the Nonsense Mediated Decay (NMD) pathway and the Integrated Stress Response (ISR). It collaborates with other proteins such as GADD34 and NEMF to manage ribosomal stalling and subsequent protein degradation. In the NMD pathway ZNF598 ensures the rapid degradation of transcripts with premature stop codons. In the ISR ZNF598's activity assists in modulating translation under conditions of cellular stress.

ZNF598's malfunction associates with neurodegenerative conditions and cancer. Mutations affecting its function can lead to translational errors contributing to the accumulation of toxic protein aggregates seen in disorders like amyotrophic lateral sclerosis (ALS). Additionally overexpression or aberrant activity of ZNF598 links to tumorigenesis as it can alter translational homeostasis. During these disease states interactions with proteins such as ATXN2 and PIK3CA emphasize the critical nature of maintaining translational integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, leading to degradation of nascent peptide chains (PubMed : 28065601, PubMed : 28132843, PubMed : 28685749, PubMed : 32099016, PubMed : 32579943, PubMed : 33581075). ZNF598 is activated when ribosomes are stalled within an mRNA following translation of prematurely polyadenylated mRNAs (PubMed : 28065601, PubMed : 28132843, PubMed : 28685749). Acts as a ribosome collision sensor : specifically recognizes and binds collided di-ribosome, which arises when a trailing ribosome encounters a slower leading ribosome, leading to terminally arrest translation (PubMed : 28065601, PubMed : 28132843, PubMed : 28685749, PubMed : 30293783). Following binding to colliding ribosomes, mediates monoubiquitination of 40S ribosomal proteins RPS10/eS10 and RPS3/uS3, and 'Lys-63'-linked polyubiquitination of RPS20/uS10 (PubMed : 28065601, PubMed : 28132843, PubMed : 28685749). Polyubiquitination of RPS20/uS10 promotes recruitment of the RQT (ribosome quality control trigger) complex, which drives the disassembly of stalled ribosomes, followed by degradation of nascent peptides (PubMed : 32099016, PubMed : 32579943, PubMed : 36302773). E3 ubiquitin-protein ligase activity is dependent on the E2 ubiquitin-conjugating enzyme UBE2D3 (PubMed : 28685749). Also acts as an adapter that recruits the 4EHP-GYF2 complex to mRNAs (PubMed : 22751931, PubMed : 32726578). Independently of its role in RQC, may also act as a negative regulator of interferon-stimulated gene (ISG) expression (PubMed : 29719242).. (Microbial infection) Required for poxvirus protein synthesis by mediating ubiquitination of RPS10/eS10 and RPS20/uS10 (PubMed : 29719242). Poxvirus encoding mRNAs contain unusual 5' poly(A) leaders and ZNF598 is required for their translational efficiency, possibly via its ability to suppress readthrough or sliding on shorter poly(A) tracts (PubMed : 29719242).
See full target information ZNF598
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:1932-1946 PubMed40646310

2025

Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Geraldine Rodschinka,Sergio Forcelloni,Felix M Kühner,Sascha Wani,Henrick Riemenschneider,Dieter Edbauer,Andrew Behrens,Danny D Nedialkova

Nature communications 8:16056 PubMed28685749

2017

The E3 ubiquitin ligase and RNA-binding protein ZNF598 orchestrates ribosome quality control of premature polyadenylated mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Aitor Garzia,Seyed Mehdi Jafarnejad,Cindy Meyer,Clément Chapat,Tasos Gogakos,Pavel Morozov,Mehdi Amiri,Maayan Shapiro,Henrik Molina,Thomas Tuschl,Nahum Sonenberg
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