Rabbit Polyclonal ZNF638 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF638 aa 1-350.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZNF638 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q14966
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
Transcription factor that binds to cytidine clusters in double-stranded DNA (PubMed:30487602, PubMed:8647861). Plays a key role in the silencing of unintegrated retroviral DNA: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602). Mediates transcriptional repression of unintegrated viral DNA by specifically binding to the cytidine clusters of retroviral DNA and mediating the recruitment of chromatin silencers, such as the HUSH complex, SETDB1 and the histone deacetylases HDAC1 and HDAC4 (PubMed:30487602). Acts as an early regulator of adipogenesis by acting as a transcription cofactor of CEBPs (CEBPA, CEBPD and/or CEBPG), controlling the expression of PPARG and probably of other proadipogenic genes, such as SREBF1 (By similarity). May also regulate alternative splicing of target genes during adipogenesis (By similarity).
Alternative names
NP220, ZFML, ZNF638, Zinc finger protein 638, Cutaneous T-cell lymphoma-associated antigen se33-1, Nuclear protein 220, Zinc finger matrin-like protein, CTCL-associated antigen se33-1
Recommended products
Rabbit Polyclonal ZNF638 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF638 aa 1-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZNF638 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q14966
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNF638 also known as ZNF638 zinc finger protein is a nuclear protein weighing approximately 197 kDa. This protein features multiple zinc finger domains which are characteristic structural motifs that enable binding to nucleic acids. Researchers find ZNF638 expressed across various tissues with high levels in the brain and testis. It functions as a regulator of gene transcription influencing the expression of numerous genes involved in cell differentiation and proliferation.
- Biological function summary
ZNF638 plays an important role in coordinating the cellular processes necessary for adipogenesis and neural development. It acts as a transcription factor and shows interaction with several other transcriptional regulators. ZNF638 also forms part of larger protein complexes involved in the modulation of chromatin structure which is important for its role in transcription regulation. By facilitating chromatin remodeling ZNF638 allows access to gene targets necessary for specific cell lineages development.
- Pathways
The functional activities of ZNF638 influence the PPAR signaling pathway which is essential for the regulation of adipocyte differentiation. The protein interacts with PPARγ an important regulator within this pathway as well as with other PPAR-associated proteins such as C/EBPα. Additionally ZNF638 involvement is evident in the regulation of the Wnt signaling pathway where it interacts with LEF1/T-cell factor to modulate the transcriptional activity involved in cell fate determination during embryonic development and tissue homeostasis.
- Associated diseases and disorders
Abnormal expression or function of ZNF638 has been linked to metabolic disorders like obesity and potentially schizophrenia. Dysregulation in adipogenesis due to altered ZNF638 activity can contribute to obesity through its interaction with proteins like PPARγ. In the context of schizophrenia variations in the ZNF638 gene expression or activity might affect neural development interacting with proteins like DISC1 a protein associated with cognitive functioning and psychiatric disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-ZNF638 antibody (ab183771)
5% SDS-PAGE
All lanes: Western blot - Anti-ZNF638 antibody (ab183771) at 1/500 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
Predicted band size: 221 kDa
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