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AB117777

Anti-ZNF644 antibody

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(1 Publication)

Rabbit Polyclonal ZNF644 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ZNF644 aa 1-50.

View Alternative Names

KIAA1221, ZEP2, ZNF644, Zinc finger protein 644, Zinc finger motif enhancer-binding protein 2, Zep-2

2 Images
Immunoprecipitation - Anti-ZNF644 antibody (AB117777)
  • IP

Unknown

Immunoprecipitation - Anti-ZNF644 antibody (AB117777)

Detection of ZNF644 by Western Blot of Immunprecipitate.
ab117777 at 1µg/ml staining ZNF644 in 293T whole cell lysate immunoprecipitated using ab117777 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ZNF644 antibody (ab117777)

Predicted band size: 150 kDa

false

Western blot - Anti-ZNF644 antibody (AB117777)
  • WB

Unknown

Western blot - Anti-ZNF644 antibody (AB117777)

All lanes:

Western blot - Anti-ZNF644 antibody (ab117777) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 150 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ZNF644 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9H582

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab117777 was affinity purified using an epitope specific to ZNF644 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF644 also known as Zinc Finger Protein 644 is a transcription factor with a molecular mass of approximately 145 kDa. It contains multiple C2H2-type zinc finger domains that facilitate binding to specific DNA sequences influencing gene expression. Researchers have found ZNF644 expression predominantly in the brain particularly in the cerebral cortex as well as in other tissues like the liver and kidney. The protein functions in regulating various genes that guide neural development and other essential cellular processes.
Biological function summary

Zinc finger proteins like ZNF644 serve as important regulators of genetic transcription. ZNF644 operates in coordination with other protein complexes to control the expression patterns critical for maintaining cellular homeostasis. The protein aids in modulating several genes involved in neurodevelopment and synaptic functions playing significant roles in the proper formation and functionality of neural circuits. Its ability to influence gene expression patterns contributes to its involvement in broader regulatory networks within the cell.

Pathways

ZNF644 is involved in gene regulatory networks that influence developmental and neuronal pathways. It interacts with other transcription factors and co-regulators like REST (RE1-Silencing Transcription Factor) that modulate neural gene expression during development. ZNF644 plays a role in the mTOR signaling pathway which is critical for cellular growth metabolism and synaptic plasticity. This involvement signifies its contribution to the maintenance of neurological health and development.

Researchers have linked ZNF644 to high myopia and certain neurodevelopmental disorders. Mutations in ZNF644 associate with refractive errors leading to high myopia impacting retinal development and eye function. Additionally the protein interacts with other neurodevelopmental proteins like CTNND2 suggesting a role in mental and cognitive disorders. Further investigation into its mutations and expression patterns could provide insights into the development and progression of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information ZNF644
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 40:523-536.e6 PubMed28350986

2017

Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Nakajima,Kimiko Yamamoto,Sobhika Agarwala,Kenta Terai,Hajime Fukui,Shigetomo Fukuhara,Koji Ando,Takahiro Miyazaki,Yasuhiro Yokota,Etienne Schmelzer,Heinz-Georg Belting,Markus Affolter,Virginie Lecaudey,Naoki Mochizuki
View all publications
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Product promise

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