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AB250194

Anti-ZNF699 antibody [EPR13383] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ZNF699 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Zinc finger protein 699, Hangover homolog, ZNF699

2 Images
Western blot - Anti-ZNF699 antibody [EPR13383] - BSA and Azide free (AB250194)
  • WB

Supplier Data

Western blot - Anti-ZNF699 antibody [EPR13383] - BSA and Azide free (AB250194)

This data was developed using ab180491, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNF699 antibody [EPR13383] (<a href='/en-us/products/primary-antibodies/znf699-antibody-epr13383-ab180491'>ab180491</a>) at 1/5000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg with immunizing peptide

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 80 kDa

false

Western blot - Anti-ZNF699 antibody [EPR13383] - BSA and Azide free (AB250194)
  • WB

Supplier Data

Western blot - Anti-ZNF699 antibody [EPR13383] - BSA and Azide free (AB250194)

This data was developed using ab180491, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNF699 antibody [EPR13383] (<a href='/en-us/products/primary-antibodies/znf699-antibody-epr13383-ab180491'>ab180491</a>) at 1/20000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution

Predicted band size: 73 kDa

Observed band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13383

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250194 is the carrier-free version of ab180491.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF699 also known as Zinc Finger Protein 699 is a protein encoded by the ZNF699 gene in humans. This protein consists of zinc finger motifs which are small protein structural motifs able to bind to DNA. The molecular mass of ZNF699 is approximately 90 kDa. It is expressed in various tissues with high levels observed in the brain liver and kidney. The primary function of ZNF699’s zinc finger domains involves binding to specific DNA sequences influencing transcriptional regulation of target genes.
Biological function summary

Zinc finger proteins like ZNF699 play important roles in gene expression. ZNF699 does not appear to be part of any large multiprotein complex but interacts with a variety of other molecules involved in transcriptional regulation. It acts by binding to regulatory regions of genes influencing the transcription process. This interaction directly affects cellular functions such as proliferation differentiation and response to external signals. The precise biological role of ZNF699 remains under continued investigation but existing studies suggest a regulatory function in various cellular processes.

Pathways

ZNF699 is involved in transcriptional regulatory pathways that govern gene expression. It participates particularly in the Wnt signaling pathway which plays a significant role in embryonic development and cell growth. ZNF699’s interaction with other transcription factors such as TCF/LEF highlights its contribution in modulating gene expression within this signaling pathway. Additionally ZNF699 may cross-talk with the Hedgehog signaling pathway via interactions that influence cell fate decisions and morphogenesis.

Researchers associate ZNF699 with certain cancers and developmental disorders. Its dysregulation may contribute to tumorigenesis influencing the expression of oncogenes and tumor suppressor genes. In some cancers ZNF699 may interact aberrantly with proteins like p53 which is a well-known tumor suppressor. Additionally alterations in ZNF699 expression may also be linked to congenital malformations potentially through disrupted regulatory networks involving proteins like GLI1 a Hedgehog pathway component. Continued research will help to define the precise role of ZNF699 in these conditions and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information Zinc finger protein 699
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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