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AB122290

Anti-ZNF740 antibody

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(1 Publication)

Rabbit Polyclonal ZNF740 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF740 aa 1-100.

View Alternative Names

TB7, ZNF740, Zinc finger protein 740, OriLyt TD-element-binding protein 7

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZNF740 antibody (AB122290)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZNF740 antibody (AB122290)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab122290 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF740 antibody (AB122290)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF740 antibody (AB122290)

ab122290, at a 1/50 dilution, staining ZNF740 in paraffin embedded Human urinary bladder tissue by Immunohistochemistry.

Western blot - Anti-ZNF740 antibody (AB122290)
  • WB

Unknown

Western blot - Anti-ZNF740 antibody (AB122290)

Lane 1 : Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1 : Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

All lanes:

Western blot - Anti-ZNF740 antibody (ab122290)

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ZNF740 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8NDX6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Zinc Finger Protein 740 (ZNF740) a member of the zinc finger family acts as a DNA-binding protein. It facilitates transcriptional regulation by interacting with specific DNA sequences. ZNF740 also known as Zinc Finger Protein 740 has an approximate molecular mass of 70 kDa. This protein exhibits expression across various tissues but shows higher levels in the brain and skeletal muscle.
Biological function summary

The Zinc Finger Protein 740 influences gene expression and cellular differentiation. ZNF740 can participate in transcriptional regulation activities as part of a larger protein complex. It interacts with transcriptional co-factors to either activate or repress target genes affecting developmental and physiological processes in the organism.

Pathways

Zinc Finger Protein 740 engages in regulatory networks that manage gene transcription. ZNF740 has connections with pathways like signal transduction and development regulation. It shows interaction with proteins such as P53 and other zinc finger proteins which collaborate in modulating genetic transcription and cellular responses.

Zinc Finger Protein 740 associates with cancer and neurological disorders. Research indicates that ZNF740 may contribute to tumor development by altering gene transcription in cancerous cells. Additionally irregular expression or mutation of ZNF740 could play a role in neurological conditions with other zinc finger proteins potentially involved in these pathological changes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation.
See full target information ZNF740
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 9:e99539 PubMed24927501

2014

C-terminal region of MAP7 domain containing protein 3 (MAP7D3) promotes microtubule polymerization by binding at the C-terminal tail of tubulin.

Applications

Unspecified application

Species

Unspecified reactive species

Saroj Yadav,Paul J Verma,Dulal Panda
View all publications
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Product promise

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