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AB121685

Anti-ZNF750 antibody

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(3 Publications)

Rabbit Polyclonal ZNF750 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZNF750 aa 600 to C-terminus.

View Alternative Names

Zinc finger protein 750, ZNF750

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZNF750 antibody (AB121685)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ZNF750 antibody (AB121685)

Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and vesicles. Recommended concentration of ab121685 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF750 antibody (AB121685)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF750 antibody (AB121685)

ab121685 at 1/25 dilution staining ZNF750 in Paraffin-embedded Human Vagina tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZNF750 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q32MQ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNF750 also known as zinc finger protein 750 is a transcription factor with a molecular weight of approximately 80 kDa. It contains zinc finger motifs that allow it to bind DNA and regulate gene expression. This protein is primarily expressed in the epidermis where it plays a critical role in cellular differentiation. ZNF750 acts by modulating the expression of genes involved in the maintenance and function of the skin barrier.
Biological function summary

This transcription factor influences the differentiation of keratinocytes the predominant cell type in the epidermis. ZNF750 controls the terminal differentiation programs by inducing the expression of differentiation-specific genes. It functions in part of a larger transcriptional complex interacting with other transcription factors and co-regulators to execute its functions. These interactions dictate the precise genetic output necessary for maintaining skin integrity.

Pathways

ZNF750 plays a part in the epidermal differentiation complex pathway. This pathway coordinates the transition of keratinocytes from proliferative basal cells to differentiated cells. The activity of ZNF750 closely links with other proteins like KLF4 and GRHL3 which are also important in epidermal regulation. Together these proteins form a network that supports skin development and repair processes.

Mutations or dysfunctions in ZNF750 have associations with skin-related conditions such as psoriasis and seborrheic dermatitis. Aberrant expression or mutations can disrupt normal keratinocyte differentiation leading to hyperproliferative skin disorders. ZNF750's activity connects with other factors including c-MYC which is involved in cellular proliferation highlighting its role in maintaining the balance between cell proliferation and differentiation in the epidermis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor involved in epidermis differentiation. Required for terminal epidermal differentiation : acts downstream of p63/TP63 and activates expression of late epidermal differentiation genes. Specifically binds to the promoter of KLF4 and promotes its expression.
See full target information ZNF750
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 32:1252-1265 PubMed36322143

2022

Reversing lysosome-ribosome circuit dysregulation mitigates C9FTD/ALS neurodegeneration and behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Chen Liang,Jing Wang,Qing Chang,Yuan Wang,Wei Zhang,Yuanning Du,Jotham Sadan,Jian-Fu Chen

Oncology letters 23:28 PubMed34868365

2021

Zinc-finger protein 750 mitigates malignant biological behavior of oral CSC-like cells enriched from parental CAL-27 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Xu,Hong-Li Yang,Yi-Kun Yang,Li Pan,Hai-Ying Chen

Folia histochemica et cytobiologica 58:255-263 PubMed33185885

2020

ZNF750 inhibits the proliferation and invasion of melanoma cells through modulating the Wnt/b-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Du,Guozhong Lv,Changrui Jing,Junjie Liu,Jing Liu
View all publications
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Product promise

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