Rabbit Recombinant Monoclonal ZNFN1A2/HELIOS antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (AB252411), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr23200-40-ab252411--western-blot-img174013.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (AB252411)")
![Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (AB252411), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr23200-40-ab252411--western-blot-img166078.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (AB252411)")
![Immunoprecipitation - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (AB252411), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr23200-40-ab252411--immunoprecipitation-img173364.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (AB252411)")
![Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (AB252411), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr23200-40-ab252411--western-blot-img167956.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (AB252411)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Expected | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Associates with Ikaros at centromeric heterochromatin.
Alternative names
HELIOS, ZNFN1A2, IKZF2, Zinc finger protein Helios, Ikaros family zinc finger protein 2
Recommended products
Rabbit Recombinant Monoclonal ZNFN1A2/HELIOS antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR23200-40
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNFN1A2 also known as HELIOS is a transcription factor with an estimated mass of approximately 65 to 70 kDa. It is a member of the Ikaros family of zinc finger proteins which are essential in regulating gene expression. HELIOS is expressed mainly in the thymus with significant levels also found in peripheral T cells. This protein plays a role in lymphocyte development and differentiation influencing the function and survival of T cells.
- Biological function summary
ZNFN1A2 performs critical functions in maintaining T cell homeostasis and promoting regulatory T cell development. It forms part of transcriptional complexes that regulate gene expression necessary for immune response modulation. ZNFN1A2 interacts with other members of the Ikaros family sharing overlapping roles in immune cell regulation which highlights its importance in immune regulation.
- Pathways
ZNFN1A2 integrates into critical signaling networks related to T cell regulation particularly the T cell receptor (TCR) signaling pathway. Within these pathways HELIOS collaborates with proteins such as FOXP3 and GATA3 to mediate immune tolerance and maintain immune system balance. These interactions underline the protein's involvement in modulating immune responses and preventing autoimmunity.
- Associated diseases and disorders
ZNFN1A2 demonstrates significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where dysregulation leads to improper T cell function. Furthermore it is implicated in leukemias particularly T-cell acute lymphoblastic leukemia (T-ALL) due to altered expression affecting T cell maturation. In these diseases ZNFN1A2 often interacts with proteins like AIRE and BCL11B influencing disease progression and clinical outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr23200-40-ab252411--western-blot-img174013.jpg "Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)")
Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1-5: 125 seconds Lane 6-8: 3 minutes
Negative control: spleen, liver, kidney, brain (PMID: 10541817)
All lanes: Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411) at 1/1000 dilution
Lane 1: Mouse thymus tissue lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse kidney tissue lysate at 20 µg
Lane 5: Mouse brain tissue lysate at 20 µg
Lane 6: Rat thymus tissue lysate at 20 µg
Lane 7: Rat spleen tissue lysate at 20 µg
Lane 8: Rat kidney tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa
![Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr23200-40-ab252411--western-blot-img166078.jpg "Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)")
Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)
Blocking and diluting buffer and concentration: 5%
NFDM/TBSTNegative control: Raw264.7 (PMID: 9512513 )
Exposure time: 3 minutes
All lanes: Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411) at 1/1000 dilution
Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa
![Immunoprecipitation - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr23200-40-ab252411--immunoprecipitation-img173364.jpg "Immunoprecipitation - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)")
Immunoprecipitation - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)
ZNFN1A2/HELIOS was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug with ab252411 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252411 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug
Lane 2: ab252411 IP in EL4 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab252411 in EL4 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3.25 seconds
All lanes: Immunoprecipitation - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)
Predicted band size: 60 kDa
![Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr23200-40-ab252411--western-blot-img167956.jpg "Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)")
Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 103 seconds
All lanes: Western blot - Anti-ZNFN1A2/HELIOS antibody [EPR23200-40] (ab252411) at 1/1000 dilution
All lanes: Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa
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