Rabbit Recombinant Monoclonal ZNFN1A2/HELIOS antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-ZNFN1A2/HELIOS antibody [EPR28015-21] - BSA and Azide free (Capture) (AB313813), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr28015-21-bsa-and-azide-free-capture-ab313813--sandwich-elisa-img419285.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-ZNFN1A2/HELIOS antibody [EPR28015-21] - BSA and Azide free (Capture) (AB313813)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Associates with Ikaros at centromeric heterochromatin.
Alternative names
Zinc finger protein Helios, Ikaros family zinc finger protein 2, IKZF2, HELIOS, ZNFN1A2
Recommended products
Rabbit Recombinant Monoclonal ZNFN1A2/HELIOS antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Zinc finger protein Helios, Ikaros family zinc finger protein 2, IKZF2, HELIOS, ZNFN1A2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR28015-21
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab313813 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ZNFN1A2/HELIOS. The recommended pair for sandwich ELISA is:
Capture: ab313813, Human ZNFN1A2/HELIOS Capture Antibody (unconjugated)
Detector: Anti-ZNFN1A2/HELIOS antibody [EPR28015-91] - BSA and Azide free (Detector) ab313814, Human ZNFN1A2/HELIOS Detector Antibody (unconjugated)
The reference range value is 15.63-2000 pg/ml.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNFN1A2 also known as HELIOS is a transcription factor with an estimated mass of approximately 65 to 70 kDa. It is a member of the Ikaros family of zinc finger proteins which are essential in regulating gene expression. HELIOS is expressed mainly in the thymus with significant levels also found in peripheral T cells. This protein plays a role in lymphocyte development and differentiation influencing the function and survival of T cells.
- Biological function summary
ZNFN1A2 performs critical functions in maintaining T cell homeostasis and promoting regulatory T cell development. It forms part of transcriptional complexes that regulate gene expression necessary for immune response modulation. ZNFN1A2 interacts with other members of the Ikaros family sharing overlapping roles in immune cell regulation which highlights its importance in immune regulation.
- Pathways
ZNFN1A2 integrates into critical signaling networks related to T cell regulation particularly the T cell receptor (TCR) signaling pathway. Within these pathways HELIOS collaborates with proteins such as FOXP3 and GATA3 to mediate immune tolerance and maintain immune system balance. These interactions underline the protein's involvement in modulating immune responses and preventing autoimmunity.
- Associated diseases and disorders
ZNFN1A2 demonstrates significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where dysregulation leads to improper T cell function. Furthermore it is implicated in leukemias particularly T-cell acute lymphoblastic leukemia (T-ALL) due to altered expression affecting T cell maturation. In these diseases ZNFN1A2 often interacts with proteins like AIRE and BCL11B influencing disease progression and clinical outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-ZNFN1A2/HELIOS antibody [EPR28015-21] - BSA and Azide free (Capture) (ab313813), expandable thumbnail](https://content.abcam.com/products/images/znfn1a2-helios-antibody-epr28015-21-bsa-and-azide-free-capture-ab313813--sandwich-elisa-img419285.jpg "Sandwich ELISA - Anti-ZNFN1A2/HELIOS antibody [EPR28015-21] - BSA and Azide free (Capture) (ab313813)")
Sandwich ELISA - Anti-ZNFN1A2/HELIOS antibody [EPR28015-21] - BSA and Azide free (Capture) (ab313813)
Sandwich ELISA of Human ZNFN1A2/HELIOS Antibody Pair - BSA and Azide free ab313812 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Downloads
Product protocols
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