JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB179452

Anti-ZNFX1 antibody [EPR12330]

Be the first to review this product! Submit a review

|

(8 Publications)

Rabbit Recombinant Monoclonal ZNFX1 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

KIAA1404, ZNFX1, NFX1-type zinc finger-containing protein 1

2 Images
Western blot - Anti-ZNFX1 antibody [EPR12330] (AB179452)
  • WB

Supplier Data

Western blot - Anti-ZNFX1 antibody [EPR12330] (AB179452)

All lanes:

Western blot - Anti-ZNFX1 antibody [EPR12330] (ab179452) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 220 kDa

false

Western blot - Anti-ZNFX1 antibody [EPR12330] (AB179452)
  • WB

Unknown

Western blot - Anti-ZNFX1 antibody [EPR12330] (AB179452)

All lanes:

Western blot - Anti-ZNFX1 antibody [EPR12330] (ab179452) at 1/1000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 220 kDa

Observed band size: 220 kDa

false

  • Carrier free

    Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12330

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The zinc finger NFX1-type containing 1 (ZNFX1) protein is a DNA-binding protein involved in nucleic acid sensing and immune regulation. It is also known as ZNF-X1. ZNFX1 has a mass of approximately 119 kDa. It expresses in various tissues with notably higher expression levels in the spleen lymph nodes and blood cells. The protein features zinc finger domains which facilitate its interaction with nucleic acids enabling ZNFX1 to play a role in transcriptional regulation and the antiviral response.
Biological function summary

ZNFX1 influences immune function and viral defense mechanisms. Acting within ribonucleoprotein complexes it participates in the sensing of double-stranded RNA an indicator of viral infection. ZNFX1 helps modulate type I interferon responses a critical aspect of host defense. In these processes it interacts with other proteins necessary for the immune system contributing to maintaining cellular immune surveillance and controlling viral replication.

Pathways

The actions of ZNFX1 integrate into pathways related to RNA processing and immune responses. Specifically ZNFX1 is involved in the RIG-I-like receptor (RLR) pathway which is important for antiviral responses. In this pathway ZNFX1 interacts with proteins like RIG-I and MAVS to facilitate the proper functioning of antiviral signaling cascades. This pathway's coordination leads to the activation of transcription factors that promote the expression of type I interferons and other cytokines essential for antiviral immunity.

ZNFX1 associates with immunological conditions including systemic lupus erythematosus (SLE). Abnormalities in ZNFX1 function or expression may contribute to the dysregulation of immune response observed in this autoimmune disease. Furthermore ZNFX1 connects with proteins such as TREX1 known to be involved in the pathogenesis of SLE through the management of immune signaling and prevention of excessive immune activation. Understanding these relationships highlights the significance of ZNFX1 in both normal and disease states of the immune system.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA-binding protein that initiates the antiviral response and is required to restrict the replication of RNA viruses (PubMed : 33872655). Acts as a double-stranded RNA (dsRNA) sensor that recognizes viral RNA and then interacts with MAVS to initiate the type I interferon response (By similarity). Also required for immunity against some bacteria, such as mycobacteria (PubMed : 33876776).
See full target information ZNFX1
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:5469-5493 PubMed39333773

2024

The human disease-associated gene ZNFX1 controls inflammation through inhibition of the NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Huang,Yao Wang,Xin Jia,Changfeng Zhao,Meiqi Zhang,Mi Bao,Pan Fu,Cuiqin Cheng,Ruona Shi,Xiaofei Zhang,Jun Cui,Gang Wan,Anlong Xu

Journal of hematology & oncology 17:77 PubMed39218923

2024

Novel potent molecular glue degraders against broad range of hematological cancer cell lines via multiple neosubstrates degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyun Li,Xiaotong Hu,Zhiya Fan,Shiyang Sun,Qijie Ran,Ting Wei,Pengli Wei,Qiyu Jiang,Jian Yan,Ning Yang,Changkai Jia,Tingting Yang,Yaqiu Mao,Xu Cai,Tingting Xu,Zhiyuan Zhao,Xiaohong Qian,Weijie Qin,Xiaomei Zhuang,Feng Fan,Junhai Xiao,Zhibing Zheng,Song Li

JCI insight 9: PubMed38016036

2023

ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing Prkaa2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Honglin Liu,Zhenyu Han,Liru Chen,Jing Zhang,Zhanqing Zhang,Yaoxin Chen,Feichang Liu,Ke Wang,Jieyu Liu,Na Sai,Xinying Zhou,Chaoying Zhou,Shengfeng Hu,Qian Wen,Li Ma

Frontiers in immunology 14:1280668 PubMed37901206

2023

Optimal temperature for the long-term culture of adult porcine islets for xenotransplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Naoaki Sakata,Gumpei Yoshimatsu,Ryo Kawakami,Chikao Aoyagi,Shohta Kodama

Journal of medical virology 95:e28637 PubMed36892175

2023

ZNFX1 antisense RNA1 promotes antiviral innate immune responses via modulating ZNFX1 function.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Jia,Meiqi Zhang,Haojia Wang,Cuiqin Cheng,Qiqi Li,Yiying Li,Lingdong Kong,Xihong Lan,Yuxi Wang,Xue Liang,Shaochun Yuan,Yao Wang,Anlong Xu

Viruses 14: PubMed35891387

2022

Sequence-Specific Features of Short Double-Strand, Blunt-End RNAs Have RIG-I- and Type 1 Interferon-Dependent or -Independent Anti-Viral Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Abhilash Kannan,Maarit Suomalainen,Romain Volle,Michael Bauer,Marco Amsler,Hung V Trinh,Stefano Vavassori,Jana Pachlopnik Schmid,Guilherme Vilhena,Alberto Marín-González,Ruben Perez,Andrea Franceschini,Christian von Mering,Silvio Hemmi,Urs F Greber

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33876776

2021

Inherited deficiency of stress granule ZNFX1 in patients with monocytosis and mycobacterial disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Le Voyer,Anna-Lena Neehus,Rui Yang,Masato Ogishi,Jérémie Rosain,Fayhan Alroqi,Maha Alshalan,Sophie Blumental,Fatima Al Ali,Taushif Khan,Manar Ata,Laurence Rozen,Anne Demulder,Paul Bastard,Conor Gruber,Manon Roynard,Yoann Seeleuthener,Franck Rapaport,Benedetta Bigio,Maya Chrabieh,Danielle Sng,Laureline Berteloot,Nathalie Boddaert,Flore Rozenberg,Saleh Al-Muhsen,Aida Bertoli-Avella,Laurent Abel,Dusan Bogunovic,Nico Marr,Davood Mansouri,Fuad Al Mutairi,Vivien Béziat,Dominique Weil,Seyed Alireza Mahdaviani,Alina Ferster,Shen-Ying Zhang,Bruno Reversade,Stéphanie Boisson-Dupuis,Jean-Laurent Casanova,Jacinta Bustamante

International journal of oncology 55:896-904 PubMed31432148

2019

Characterization of the human zinc finger nfx‑1‑type containing 1 encoding ZNFX1 gene and its response to 12‑O‑tetradecanoyl‑13‑acetate in HL‑60 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Hamada,Mayu Yamamura,Hiroto Ohi,Yota Kobayashi,Kuniyoshi Niwa,Takahiro Oyama,Yasunari Mano,Masashi Asai,Sei-Ichi Tanuma,Fumiaki Uchiumi
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB111565

Anti-PI4KA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI4KA antibody (AB111565)

  • 0
  • 0
Primary Antibodies

AB223759

Anti-p115-RhoGEF antibody

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p115-RhoGEF antibody (AB223759)

  • 0
  • 0
Primary Antibodies

AB2244

Anti-RAC2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAC2 antibody (AB2244)

  • 0
  • 0
Primary Antibodies

AB167429

Anti-KIF5B+KIF5C antibody [EPR10276(B)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-KIF5B+KIF5C antibody [EPR10276(B)] (AB167429)

  • 4
  • 3

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com