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AB250095

Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free

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Rabbit Recombinant Monoclonal ZNFX1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

KIAA1404, ZNFX1, NFX1-type zinc finger-containing protein 1

2 Images
Western blot - Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free (AB250095)
  • WB

Supplier Data

Western blot - Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free (AB250095)

This data was developed using ab179452, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNFX1 antibody [EPR12330] (<a href='/en-us/products/primary-antibodies/znfx1-antibody-epr12330-ab179452'>ab179452</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 220 kDa

false

Western blot - Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free (AB250095)
  • WB

Unknown

Western blot - Anti-ZNFX1 antibody [EPR12330] - BSA and Azide free (AB250095)

This data was developed using ab179452, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ZNFX1 antibody [EPR12330] (<a href='/en-us/products/primary-antibodies/znfx1-antibody-epr12330-ab179452'>ab179452</a>) at 1/1000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 220 kDa

Observed band size: 220 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12330

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250095 is the carrier-free version of ab179452.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The zinc finger NFX1-type containing 1 (ZNFX1) protein is a DNA-binding protein involved in nucleic acid sensing and immune regulation. It is also known as ZNF-X1. ZNFX1 has a mass of approximately 119 kDa. It expresses in various tissues with notably higher expression levels in the spleen lymph nodes and blood cells. The protein features zinc finger domains which facilitate its interaction with nucleic acids enabling ZNFX1 to play a role in transcriptional regulation and the antiviral response.
Biological function summary

ZNFX1 influences immune function and viral defense mechanisms. Acting within ribonucleoprotein complexes it participates in the sensing of double-stranded RNA an indicator of viral infection. ZNFX1 helps modulate type I interferon responses a critical aspect of host defense. In these processes it interacts with other proteins necessary for the immune system contributing to maintaining cellular immune surveillance and controlling viral replication.

Pathways

The actions of ZNFX1 integrate into pathways related to RNA processing and immune responses. Specifically ZNFX1 is involved in the RIG-I-like receptor (RLR) pathway which is important for antiviral responses. In this pathway ZNFX1 interacts with proteins like RIG-I and MAVS to facilitate the proper functioning of antiviral signaling cascades. This pathway's coordination leads to the activation of transcription factors that promote the expression of type I interferons and other cytokines essential for antiviral immunity.

ZNFX1 associates with immunological conditions including systemic lupus erythematosus (SLE). Abnormalities in ZNFX1 function or expression may contribute to the dysregulation of immune response observed in this autoimmune disease. Furthermore ZNFX1 connects with proteins such as TREX1 known to be involved in the pathogenesis of SLE through the management of immune signaling and prevention of excessive immune activation. Understanding these relationships highlights the significance of ZNFX1 in both normal and disease states of the immune system.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that initiates the antiviral response and is required to restrict the replication of RNA viruses (PubMed : 33872655). Acts as a double-stranded RNA (dsRNA) sensor that recognizes viral RNA and then interacts with MAVS to initiate the type I interferon response (By similarity). Also required for immunity against some bacteria, such as mycobacteria (PubMed : 33876776).
See full target information ZNFX1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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