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AB126133

Anti-ZNHIT2 antibody

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(2 Publications)

Rabbit Polyclonal ZNHIT2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ZNHIT2 aa 1-250.

View Alternative Names

C11orf5, ZNHIT2, Zinc finger HIT domain-containing protein 2, Protein FON

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ZNHIT2 antibody (AB126133)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZNHIT2 antibody (AB126133)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNHIT2 (green) using ab126133 at 1/500 dilution in ICC/IF. The lower panel is costained with Hoechst 33342.

Western blot - Anti-ZNHIT2 antibody (AB126133)
  • WB

Unknown

Western blot - Anti-ZNHIT2 antibody (AB126133)

All lanes:

Western blot - Anti-ZNHIT2 antibody (ab126133) at 1/1000 dilution

All lanes:

Mouse testis whole cell lysate at 50 µg

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ZNHIT2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UHR6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNHIT2 also known as Zinc Finger HIT-Type Containing 2 is a protein that plays a role in transcription regulation by modulating the activity of other proteins. With a molecular mass of approximately 31 kDa this protein is characterized by the presence of a zinc-finger HIT domain. ZNHIT2 is expressed in various tissues including the brain testis and heart indicating its involvement in diverse physiological processes. Its expression pattern suggests a significant role in cellular activities affecting many tissue types.
Biological function summary

ZNHIT2 participates in the assembly and function of essential ribonucleoprotein complexes. It acts as a facilitator in the modification of these complexes ensuring proper ribosome maturation and function. ZNHIT2 is part of larger multi-subunit complexes that include proteins from the U3 snoRNP complex impacting the processing of precursor rRNA. Its presence in these complexes highlights its involvement in maintaining the efficiency of cellular protein synthesis mechanisms.

Pathways

ZNHIT2 plays a role in the ribosome biogenesis pathway. It directly interacts with other proteins like NOLC1 and BOP1 which are important for the production of functional ribosomes. These interactions ensure the proper progression and regulation of ribosomal assembly and function making ZNHIT2 an important player in the protein synthesis pathway. By coordinating with these proteins ZNHIT2 helps maintain cellular homeostasis and protein production.

Alterations in ZNHIT2 expression have been implicated in neurodegenerative diseases and certain cancers. ZNHIT2's interaction with proteins such as NOP56 and DDX21 links it to disorders like spinocerebellar ataxias (SCAs) where ribosome biogenesis is disrupted. Additionally in some cancers ZNHIT2 expression levels may affect tumor progression due to its influence on cellular growth mechanisms. Understanding how ZNHIT2 functions in these contexts can provide insights into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a bridging factor mediating the interaction between the R2TP/Prefoldin-like (R2TP/PFDL) complex and U5 small nuclear ribonucleoprotein (U5 snRNP) (PubMed : 28561026). Required for the interaction of R2TP complex subunit RPAP3 and prefoldin-like subunit URI1 with U5 snRNP proteins EFTUD2 and PRPF8 (PubMed : 28561026). May play a role in regulating the composition of the U5 snRNP complex (PubMed : 28561026).
See full target information ZNHIT2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e12427 PubMed38545803

2024

The small extracellular vesicle-mediated intercellular transformation of CXCR1 to CXCR1 tumour cells promotes the progression of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoshi Xu,Aoming Cheng,Bo Li,Xutengyue Tian,Zhengxue Han,Zhien Feng

Nucleic acids research 50:1128-1146 PubMed34951455

2021

CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Serna,Ana González-Corpas,Sofía Cabezudo,Andrés López-Perrote,Gianluca Degliesposti,Eduardo Zarzuela,J Mark Skehel,Javier Muñoz,Oscar Llorca
View all publications
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Product promise

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