Rabbit Polyclonal ZNHIT2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZNHIT2 aa 1-250.
Images
Publications
Filter by
- Nucleic acids research 50:1128-11462021CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Serna,Ana González-Corpas,Sofía Cabezudo,Andrés López-Perrote,Gianluca Degliesposti,Eduardo Zarzuela,J Mark Skehel,Javier Muñoz,Oscar LlorcaPubMed 34951455
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZNHIT2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHR6
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
May act as a bridging factor mediating the interaction between the R2TP/Prefoldin-like (R2TP/PFDL) complex and U5 small nuclear ribonucleoprotein (U5 snRNP) (PubMed:28561026). Required for the interaction of R2TP complex subunit RPAP3 and prefoldin-like subunit URI1 with U5 snRNP proteins EFTUD2 and PRPF8 (PubMed:28561026). May play a role in regulating the composition of the U5 snRNP complex (PubMed:28561026).
Alternative names
C11orf5, ZNHIT2, Zinc finger HIT domain-containing protein 2, Protein FON
Recommended products
Rabbit Polyclonal ZNHIT2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZNHIT2 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZNHIT2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHR6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNHIT2 also known as Zinc Finger HIT-Type Containing 2 is a protein that plays a role in transcription regulation by modulating the activity of other proteins. With a molecular mass of approximately 31 kDa this protein is characterized by the presence of a zinc-finger HIT domain. ZNHIT2 is expressed in various tissues including the brain testis and heart indicating its involvement in diverse physiological processes. Its expression pattern suggests a significant role in cellular activities affecting many tissue types.
- Biological function summary
ZNHIT2 participates in the assembly and function of essential ribonucleoprotein complexes. It acts as a facilitator in the modification of these complexes ensuring proper ribosome maturation and function. ZNHIT2 is part of larger multi-subunit complexes that include proteins from the U3 snoRNP complex impacting the processing of precursor rRNA. Its presence in these complexes highlights its involvement in maintaining the efficiency of cellular protein synthesis mechanisms.
- Pathways
ZNHIT2 plays a role in the ribosome biogenesis pathway. It directly interacts with other proteins like NOLC1 and BOP1 which are important for the production of functional ribosomes. These interactions ensure the proper progression and regulation of ribosomal assembly and function making ZNHIT2 an important player in the protein synthesis pathway. By coordinating with these proteins ZNHIT2 helps maintain cellular homeostasis and protein production.
- Associated diseases and disorders
Alterations in ZNHIT2 expression have been implicated in neurodegenerative diseases and certain cancers. ZNHIT2's interaction with proteins such as NOP56 and DDX21 links it to disorders like spinocerebellar ataxias (SCAs) where ribosome biogenesis is disrupted. Additionally in some cancers ZNHIT2 expression levels may affect tumor progression due to its influence on cellular growth mechanisms. Understanding how ZNHIT2 functions in these contexts can provide insights into potential therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-ZNHIT2 antibody (ab126133)
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ZNHIT2 (green) using ab126133 at 1/500 dilution in ICC/IF. The lower panel is costained with Hoechst 33342.
Western blot - Anti-ZNHIT2 antibody (ab126133)
All lanes: Western blot - Anti-ZNHIT2 antibody (ab126133) at 1/1000 dilution
All lanes: Mouse testis whole cell lysate at 50 µg
Predicted band size: 43 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com