Goat Polyclonal ZNRF1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Znrf1 aa 50-150.
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Publications
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- Molecular cancer 21:242022circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChonghui Hu,Renpeng Xia,Xiang Zhang,Tingting Li,Yuancheng Ye,Guolin Li,Rihua He,Zhihua Li,Qing Lin,Shangyou Zheng,Rufu ChenPubMed 35045883
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Znrf1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q91V17
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5-10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 5-10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin-protein ligase that plays a role in different processes including cell differentiation, receptor recycling or regulation of inflammation (PubMed:28593998, PubMed:33996800, PubMed:37158982). Mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation (PubMed:22797923). Controls ligand-induced EGFR signaling via mediating receptor ubiquitination and recruitment of the ESCRT machinery (PubMed:33996800). Acts as a negative feedback mechanism controlling TLR3 trafficking by mediating TLR3 'Lys-63'-linked polyubiquitination to reduce type I IFN production (PubMed:37158982). Modulates inflammation by promoting caveolin-1/CAV1 ubiquitination and degradation to regulate TLR4-activated immune response (PubMed:28593998).
Alternative names
NIN283, ZNRF1, E3 ubiquitin-protein ligase ZNRF1, Nerve injury-induced gene 283 protein, RING-type E3 ubiquitin transferase ZNRF1, Zinc/RING finger protein 1
Recommended products
Goat Polyclonal ZNRF1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Znrf1 aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Znrf1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q91V17
- Purification technique
- Affinity purification Immunogen
- Specificity
ab175125 is expected to recognize reported isoforms a and b (NP_573469.1 and NP_001162093.1). Reported variants represent identical protein (NP_001162092.1 and NP_573469.1).
- Concentration
- Purification notes
ab175125 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZNRF1 also known as Zinc and Ring Finger 1 is an E3 ubiquitin-protein ligase weighing around 39 kDa. This protein directs the attachment of ubiquitin to target substrates marking them for degradation by the proteasome. ZNRF1 shows expression in the central nervous system and peripheral tissues indicating its significant role in cellular processes across diverse tissue types.
- Biological function summary
The cellular function of ZNRF1 involves the regulation of protein stability and turnover affecting a range of physiological processes. ZNRF1 interacts with several key signaling proteins and often functions in coordination with other ubiquitylation enzymes though not classified as part of a specific larger protein complex. Its activity is essential for modulating neuronal survival and regeneration particularly after injury or during neurodegenerative conditions.
- Pathways
The activity of ZNRF1 influences the neurotrophin signaling and Wnt pathways. It regulates the degradation of specific neuronal proteins connecting it functionally to proteins such as GAP-43 within the neurotrophin pathway. In the Wnt pathway ZNRF1 impacts protein degradation processes that affect cellular responses to extracellular signals and synaptic function.
- Associated diseases and disorders
ZNRF1 has relevance in neurodegenerative conditions for example Alzheimer's disease where its dysfunction might contribute to neuronal death. It also relates to peripheral neuropathies where the protein's dysregulation impacts nerve regeneration. ZNRF1's role in these diseases connects it with proteins like tau in Alzheimer's and ErbB receptors in neuropathies highlighting its influence on disease progression and potential therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF1 antibody (ab175125)
Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex, labeling ZNRF1 using ab175125 at 10 μg/ml. Streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary.
Western blot - Anti-ZNRF1 antibody (ab175125)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-ZNRF1 antibody (ab175125) at 1 µg/mL
Lane 1: Non-transfected N2a cells
Lane 2: Mouse Znrf1 transfected N2a cells
Lane 3: Mouse embryo brain lysate
Lane 4: Mouse adult brain lysate
Developed using the ECL technique.
Predicted band size: 24 kDa
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