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AB175125

Anti-ZNRF1 antibody

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(2 Publications)

Goat Polyclonal ZNRF1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Znrf1 aa 50-150.

View Alternative Names

NIN283, ZNRF1, E3 ubiquitin-protein ligase ZNRF1, Nerve injury-induced gene 283 protein, RING-type E3 ubiquitin transferase ZNRF1, Zinc/RING finger protein 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF1 antibody (AB175125)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF1 antibody (AB175125)

Immunohistochemical analysis of paraffin embedded Mouse cerebral cortex, labeling ZNRF1 using ab175125 at 10 μg/ml. Streptavidfine-Alexa 488-staining after biotinylated anti-goat secondary.

Western blot - Anti-ZNRF1 antibody (AB175125)
  • WB

Supplier Data

Western blot - Anti-ZNRF1 antibody (AB175125)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ZNRF1 antibody (ab175125) at 1 µg/mL

Lane 1:

Non-transfected N2a cells

Lane 2:

Mouse Znrf1 transfected N2a cells

Lane 3:

Mouse embryo brain lysate

Lane 4:

Mouse adult brain lysate

Predicted band size: 24 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Mouse Znrf1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q91V17

Specificity

ab175125 is expected to recognize reported isoforms a and b (NP_573469.1 and NP_001162093.1). Reported variants represent identical protein (NP_001162092.1 and NP_573469.1).

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Primary Antibodies

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Primary Antibodies

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Anti-LIF antibody [EPR28693-84] - BSA and Azide free

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Biochemicals

AB144641

PR 619, DUB inhibitor

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab175125 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZNRF1 also known as Zinc and Ring Finger 1 is an E3 ubiquitin-protein ligase weighing around 39 kDa. This protein directs the attachment of ubiquitin to target substrates marking them for degradation by the proteasome. ZNRF1 shows expression in the central nervous system and peripheral tissues indicating its significant role in cellular processes across diverse tissue types.
Biological function summary

The cellular function of ZNRF1 involves the regulation of protein stability and turnover affecting a range of physiological processes. ZNRF1 interacts with several key signaling proteins and often functions in coordination with other ubiquitylation enzymes though not classified as part of a specific larger protein complex. Its activity is essential for modulating neuronal survival and regeneration particularly after injury or during neurodegenerative conditions.

Pathways

The activity of ZNRF1 influences the neurotrophin signaling and Wnt pathways. It regulates the degradation of specific neuronal proteins connecting it functionally to proteins such as GAP-43 within the neurotrophin pathway. In the Wnt pathway ZNRF1 impacts protein degradation processes that affect cellular responses to extracellular signals and synaptic function.

ZNRF1 has relevance in neurodegenerative conditions for example Alzheimer's disease where its dysfunction might contribute to neuronal death. It also relates to peripheral neuropathies where the protein's dysregulation impacts nerve regeneration. ZNRF1's role in these diseases connects it with proteins like tau in Alzheimer's and ErbB receptors in neuropathies highlighting its influence on disease progression and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that plays a role in different processes including cell differentiation, receptor recycling or regulation of inflammation (PubMed : 28593998, PubMed : 33996800, PubMed : 37158982). Mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons : degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation (PubMed : 22797923). Controls ligand-induced EGFR signaling via mediating receptor ubiquitination and recruitment of the ESCRT machinery (PubMed : 33996800). Acts as a negative feedback mechanism controlling TLR3 trafficking by mediating TLR3 'Lys-63'-linked polyubiquitination to reduce type I IFN production (PubMed : 37158982). Modulates inflammation by promoting caveolin-1/CAV1 ubiquitination and degradation to regulate TLR4-activated immune response (PubMed : 28593998).
See full target information ZNRF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 21:24 PubMed35045883

2022

circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chonghui Hu,Renpeng Xia,Xiang Zhang,Tingting Li,Yuancheng Ye,Guolin Li,Rihua He,Zhihua Li,Qing Lin,Shangyou Zheng,Rufu Chen

Oncology 98:549-557 PubMed32408308

2020

High EREG Expression Is Predictive of Better Outcomes in Rectal Cancer Patients Receiving Neoadjuvant Concurrent Chemoradiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Yi Lin,Pei-Ling Hsieh,Chia-Lin Chou,Ching-Chieh Yang,Sung-Wei Lee,Yu-Feng Tian,Yow-Ling Shiue,Wan-Shan Li
View all publications
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Product promise

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