AB156011

Anti-ZNRF2 antibody [EPR11399(B)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal ZNRF2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

RNF202, ZNRF2, E3 ubiquitin-protein ligase ZNRF2, Protein Ells2, RING finger protein 202, RING-type E3 ubiquitin transferase ZNRF2, Zinc/RING finger protein 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF2 antibody [EPR11399(B)] (AB156011)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF2 antibody [EPR11399(B)] (AB156011)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ZNRF2 using ab156011 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF2 antibody [EPR11399(B)] (AB156011)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling ZNRF2 using ab156011 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ZNRF2 antibody [EPR11399(B)] (AB156011)

All lanes:

Western blot - Anti-ZNRF2 antibody [EPR11399(B)] (ab156011) at 1/1000 dilution

Lane 1:

Jurkat lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Lane 4:

Neuro-2a lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11399(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The zinc and ring finger protein 2 (ZNRF2) also known as RNF202 is an E3 ubiquitin-protein ligase with significant roles in protein regulation. It has a molecular mass of approximately 37 kDa. ZNRF2 is expressed across various tissues with higher levels found in neural tissues such as the brain. It mainly localizes in the cytoplasm and contributes to the ubiquitin-proteasome pathway aiding in protein degradation by tagging substrates with ubiquitin molecules.

Biological function summary

ZNRF2 functions as part of the cellular apparatus that manages protein homeostasis. It contains a RING finger domain which facilitates its interaction with ubiquitin-conjugating enzymes necessary for its ligase activity. While not always functioning in a complex ZNRF2 often engages with different protein partners to exert its ubiquitination roles in neurons and other cells. This activity influences various cellular processes including neural signal transduction and synaptic plasticity which are important for maintaining nerve cell function and integrity.

Pathways

ZNRF2 interfaces with the ubiquitin-proteasome system and the neural development pathways. It associates with proteins such as neurofilament light polypeptide (NEFL) and neighbors in signaling cascades involving neural growth and repair. It's essential for pathways regulating synaptic function providing a check on protein turnover which informs signal transduction pathways. Consequently its action is critical in controlling the cellular response to external stimuli and internal signals.

Dysregulation of ZNRF2 is implicated in neurodegenerative diseases particularly Alzheimer's disease. Its involvement in protein homeostasis and degradation means that any dysfunction can exacerbate the aggregation of misfolded proteins a hallmark of neurodegeneration. Additionally ZNRF2 associates with the regulation of protein processes involving Tau a protein that forms neurofibrillary tangles in Alzheimer's pathology. Understanding ZNRF2's pathways and interactions paves the way for potential therapeutic targets in treating such disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

E3 ubiquitin-protein ligase that plays a role in the establishment and maintenance of neuronal transmission and plasticity. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation (PubMed : 22797923). Acts also as a positive regulator of mTORC1 activation by amino acids, which functions upstream of the V-ATPase and of Rag-GTPases (PubMed : 27244671). In turn, phosphorylation by mTOR leads to its inhibition via targeting to the cytosol allowing a self-regulating feedback mechanism (PubMed : 27244671).

See full target information ZNRF2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 8:15502 PubMed28593998

2017

The ubiquitin ligase ZNRF1 promotes caveolin-1 ubiquitination and degradation to modulate inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Yuan Lee,Ting-Yu Lai,Meng-Kun Tsai,Yung-Chi Chang,Yu-Hsin Ho,I-Shing Yu,Tzu-Wen Yeh,Chih-Chang Chou,You-Sheng Lin,Toby Lawrence,Li-Chung Hsu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZNRF2 antibody [EPR11399(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF2 antibody [EPR11399(B)] (AB156011)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNRF2 antibody [EPR11399(B)] (AB156011)

Western blot - Anti-ZNRF2 antibody [EPR11399(B)] (AB156011)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise