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AB214320

Anti-ZnT-3 antibody

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(1 Publication)

Rabbit Polyclonal ZnT-3 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC30A3 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ZNT3, SLC30A3, Probable proton-coupled zinc antiporter SLC30A3, Solute carrier family 30 member 3, Zinc transporter 3, ZnT-3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT-3 antibody (AB214320)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT-3 antibody (AB214320)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling with ZnT-3 with ab214320 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC30A3 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q99726

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZnT-3 also known as Znt3 or SLC30A3 is a zinc transporter protein. It facilitates the transport of zinc ions out of the cytoplasm into synaptic vesicles. With a molecular weight of approximately 45 kDa ZnT-3 comprises part of the superfamily of cation diffusion facilitator proteins. This protein is predominantly expressed in the brain particularly within the hippocampus and cerebellum. Its presence in synaptically active regions emphasizes its role in neuronal function.
Biological function summary

The action of ZnT-3 in transporting zinc ions into synaptic vesicles suggests its pivotal function in synaptic transmission and neuronal signaling. Zinc acts as a neuromodulator influencing neurotransmitter release and receptor regulation. ZnT-3 operates independently and it does not form part of larger macromolecular complexes. By regulating zinc ion homeostasis within synaptic vesicles it impacts processes like neurotransmission and neuroplasticity.

Pathways

ZnT-3’s function integrates into the well-known zinc signaling pathways in neural activity. It interacts closely with proteins involved in calcium signaling such as calmodulin. These pathways contribute to synaptic plasticity and cognitive functions like learning and memory. Zinc modulators impact the activity of NMDA receptors through which ZnT-3 participates in modulating synaptic strength and plasticity.

ZnT-3 associates significantly with Alzheimer's disease and epilepsy. Alzheimer's pathology involves disrupted zinc homeostasis highlighting ZnT-3’s role. Alterations in ZnT-3 expression affect amyloid beta accumulation linking it with amyloid precursor protein. Additionally ZnT-3’s influence in synaptic zinc release suggests a relationship with seizure susceptibility in epilepsy where zinc interacts with zinc-binding proteins like metallothioneins affecting neuronal excitability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable proton-coupled zinc ion antiporter mediating the import of zinc from cytoplasm into synaptic vesicles and participating to cellular zinc ion homeostasis in the brain.
See full target information SLC30A3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 14:688050 PubMed34630032

2021

Tandem Mass Tag-Based Quantitative Proteomic Analysis Reveals Pathways Involved in Brain Injury Induced by Chest Exposure to Shock Waves.

Applications

Unspecified application

Species

Unspecified reactive species

Changci Tong,Peifang Cong,Ying Liu,Xiuyun Shi,Lin Shi,Shun Mao,Yan Zhao,Mingxiao Hou,Yunen Liu
View all publications
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