Rabbit Polyclonal ZnT-8 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC30A8 aa 300 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SLC30A8 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IWU4
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2500.00000 - 1/5000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Proton-coupled zinc ion antiporter mediating the entry of zinc into the lumen of pancreatic beta cell secretory granules, thereby regulating insulin secretion.
Alternative names
ZNT8, SLC30A8, Proton-coupled zinc antiporter SLC30A8, Solute carrier family 30 member 8, Zinc transporter 8, ZnT-8
Recommended products
Rabbit Polyclonal ZnT-8 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC30A8 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SLC30A8 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IWU4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zinc transporter 8 (ZnT-8) also known as SLC30A8 is a protein that functions as a zinc transporter located in the membrane of insulin secretory vesicles in pancreatic beta cells. The molecular mass of ZnT-8 is approximately 51 kDa. Expression of ZnT-8 has been observed predominantly in the pancreas with significant presence in islet cells. ZnT-8 facilitates the movement of zinc ions from the cytoplasm into insulin granules which is essential for proper insulin crystallization and storage.
- Biological function summary
Zinc plays an important role in insulin maturation and secretion and ZnT-8 contributes to these processes by regulating zinc ion homeostasis within the pancreatic beta cells. ZnT-8 is not part of a larger complex but operates alongside other zinc transporters to maintain zinc balance. This transporter ensures that zinc levels within insulin granules achieve conditions necessary for effective packaging and release of insulin.
- Pathways
ZnT-8 is directly involved in regulating glucose homeostasis and insulin signaling pathways. The protein plays a significant part in the insulin secretion pathway enabling the correct response to fluctuating blood glucose levels. ZnT-8 activity is closely related to glucose transporter type 2 (GLUT2) and other components of the insulin signaling pathway which ensures the maintenance of normal blood glucose levels through appropriate insulin action and secretion.
- Associated diseases and disorders
ZnT-8 has been closely associated with type 2 diabetes and autoimmune diabetes. Variants in the SLC30A8 gene which encodes ZnT-8 have been linked to altered risk of developing type 2 diabetes. This connection implicates ZnT-8 in the pathophysiology of impaired insulin function. Furthermore ZnT-8 is a significant autoantigen in autoimmune diabetes with antibodies against ZnT-8 (anti-ZnT8 antibodies) being detected in patients suggesting an immune response involving this protein in the disease process.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT-8 antibody (ab244550)
Paraffin-embedded human pancreas tissue stained for ZnT-8 using ab244550 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT-8 antibody (ab244550)
Paraffin-embedded human liver tissue stained for ZnT-8 using ab244550 at 1/2500 dilution in immunohistochemical analysis.
Low expression as expected.
Downloads
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