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AB244550

Anti-ZnT-8 antibody

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(1 Publication)

Rabbit Polyclonal ZnT-8 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC30A8 aa 300 to C-terminus.

View Alternative Names

ZNT8, SLC30A8, Proton-coupled zinc antiporter SLC30A8, Solute carrier family 30 member 8, Zinc transporter 8, ZnT-8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT-8 antibody (AB244550)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT-8 antibody (AB244550)

Paraffin-embedded human pancreas tissue stained for ZnT-8 using ab244550 at 1/2500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT-8 antibody (AB244550)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT-8 antibody (AB244550)

Paraffin-embedded human liver tissue stained for ZnT-8 using ab244550 at 1/2500 dilution in immunohistochemical analysis.

Low expression as expected.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLC30A8 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IWU4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zinc transporter 8 (ZnT-8) also known as SLC30A8 is a protein that functions as a zinc transporter located in the membrane of insulin secretory vesicles in pancreatic beta cells. The molecular mass of ZnT-8 is approximately 51 kDa. Expression of ZnT-8 has been observed predominantly in the pancreas with significant presence in islet cells. ZnT-8 facilitates the movement of zinc ions from the cytoplasm into insulin granules which is essential for proper insulin crystallization and storage.
Biological function summary

Zinc plays an important role in insulin maturation and secretion and ZnT-8 contributes to these processes by regulating zinc ion homeostasis within the pancreatic beta cells. ZnT-8 is not part of a larger complex but operates alongside other zinc transporters to maintain zinc balance. This transporter ensures that zinc levels within insulin granules achieve conditions necessary for effective packaging and release of insulin.

Pathways

ZnT-8 is directly involved in regulating glucose homeostasis and insulin signaling pathways. The protein plays a significant part in the insulin secretion pathway enabling the correct response to fluctuating blood glucose levels. ZnT-8 activity is closely related to glucose transporter type 2 (GLUT2) and other components of the insulin signaling pathway which ensures the maintenance of normal blood glucose levels through appropriate insulin action and secretion.

ZnT-8 has been closely associated with type 2 diabetes and autoimmune diabetes. Variants in the SLC30A8 gene which encodes ZnT-8 have been linked to altered risk of developing type 2 diabetes. This connection implicates ZnT-8 in the pathophysiology of impaired insulin function. Furthermore ZnT-8 is a significant autoantigen in autoimmune diabetes with antibodies against ZnT-8 (anti-ZnT8 antibodies) being detected in patients suggesting an immune response involving this protein in the disease process.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-coupled zinc ion antiporter mediating the entry of zinc into the lumen of pancreatic beta cell secretory granules, thereby regulating insulin secretion.
See full target information SLC30A8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 36:1654-1663 PubMed33969609

2021

Lysosome Fe release is responsible for etoposide- and cisplatin-induced stemness of small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kangwu Wang,Xiao Chen,Wang Zuyi,Liucheng Chen,Wei Fu
View all publications
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Product promise

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