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AB110383

Anti-ZnT1 antibody

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(1 Publication)

Rabbit Polyclonal ZNT1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC30A1.

View Alternative Names

ZNT1, SLC30A1, Proton-coupled zinc antiporter SLC30A1, Solute carrier family 30 member 1, Zinc transporter 1

1 Images
Western blot - Anti-ZnT1 antibody (AB110383)
  • WB

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Western blot - Anti-ZnT1 antibody (AB110383)

All lanes:

Western blot - Anti-ZnT1 antibody (ab110383) at 1/500 dilution

Lane 1:

Extracts from LOVO cells at 30 µg

Lane 2:

Extracts from LOVO cells at 30 µg with blocking peptide

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SLC30A1. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6M5

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Alternative Products

Primary Antibodies

AB214356

Anti-ZnT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT1 antibody (AB214356)

  • 0
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Complementary Products

Primary Antibodies

AB230121

Anti-ZIP-4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP-4 antibody (AB230121)

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Primary Antibodies

AB12228

Anti-Metallothionein antibody [UC1MT]

Western blot - Anti-Metallothionein antibody [UC1MT] (AB12228)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB55735

Anti-DMT1 antibody [4C6]

KO Validated

Western blot - Anti-DMT1 antibody [4C6] (AB55735)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ZnT1 also known as SLC30A1 is a critical protein responsible for the export of zinc ions out of cells. This transporter plays an important role in maintaining intracellular zinc homeostasis. The protein has a molecular mass of approximately 52 kDa. ZnT1 is expressed in a wide range of tissues including the brain liver and intestines indicating its significant role in various physiological processes. The protein is located in the cell plasma membrane which aligns with its function of mediating zinc efflux.
Biological function summary

ZnT1 is essential for regulating the cellular zinc levels and protecting against zinc toxicity. It does not form part of a larger complex; instead it operates as an individual unit to ensure that excess zinc does not accumulate to harmful levels within the cell. By controlling zinc efflux ZnT1 influences processes such as cell signaling and apoptosis which rely on zinc as a cofactor or structural component.

Pathways

Homeostasis of zinc ions in cells connects ZnT1 to the cellular zinc transport pathway and the metal ion transport pathway. ZnT1 coordinates with intracellular zinc-binding proteins like metallothioneins to modulate zinc availability and transportation within the cell. Proteins such as ZIP4 which facilitate zinc uptake operate in a complementary manner to ZnT1 balancing zinc influx and efflux to maintain cellular function.

Misregulation of ZnT1 has been linked to conditions such as cancer and neurodegenerative diseases. In cancer changes in zinc transport influence tumor growth and progression partly involving interactions with proteins like p53 a critical tumor suppressor modulated by zinc availability. In neurodegenerative diseases abnormal zinc levels can contribute to neuronal damage where ZnT1 interacts with Amyloid Beta plaques affecting the pathology of Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Zinc ion : proton antiporter that could function at the plasma membrane mediating zinc efflux from cells against its electrochemical gradient protecting them from intracellular zinc accumulation and toxicity (PubMed : 31471319). Alternatively, could prevent the transport to the plasma membrane of CACNB2, the L-type calcium channels regulatory subunit, through a yet to be defined mechanism. By modulating the expression of these channels at the plasma membrane, could prevent calcium and zinc influx into cells. By the same mechanism, could also prevent L-type calcium channels-mediated heavy metal influx into cells (By similarity). In some cells, could also function as a zinc ion : proton antiporter mediating zinc entry into the lumen of cytoplasmic vesicles. In macrophages, can increase zinc ions concentration into the lumen of cytoplasmic vesicles containing engulfed bacteria and could help inactivate them (PubMed : 32441444). Forms a complex with TMC6/EVER1 and TMC8/EVER2 at the ER membrane of keratynocytes which facilitates zinc uptake into the ER (PubMed : 18158319). Down-regulates the activity of transcription factors induced by zinc and cytokines (PubMed : 18158319).
See full target information SLC30A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 6:29428-39 PubMed26338969

2015

Exposure to airborne PM2.5 suppresses microRNA expression and deregulates target oncogenes that cause neoplastic transformation in NIH3T3 cells.

Applications

WB

Species

Unspecified reactive species

Chunling Liu,Huan Guo,Xinxin Cheng,Mingming Shao,Chen Wu,Suhan Wang,Hongmin Li,Lixuan Wei,Yanning Gao,Wen Tan,Shujun Cheng,Tangchun Wu,Dianke Yu,Dongxin Lin
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Product promise

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