Rabbit Polyclonal ZNT1 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC30A1 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.
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- Poultry science 102:1024772023The organic zinc with moderate chelation strength enhances the expression of related transporters in the jejunum and ileum of broilers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun Hu,Yanhui Huang,Chuanlong Wang,Weiyun Zhang,Yicheng Qu,Ding Li,Wei Wu,Feiyu Gao,Ling Zhu,Bingxin Wu,Liyang Zhang,Xiaoyan Cui,Tingting Li,Yanqiang Geng,Xiudong Liao,Xugang LuoPubMed 36680861
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC30A1 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6M5
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Predicted |
| Mouse | Tested |
| Rat | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Zinc ion:proton antiporter that could function at the plasma membrane mediating zinc efflux from cells against its electrochemical gradient protecting them from intracellular zinc accumulation and toxicity (PubMed:31471319). Alternatively, could prevent the transport to the plasma membrane of CACNB2, the L-type calcium channels regulatory subunit, through a yet to be defined mechanism. By modulating the expression of these channels at the plasma membrane, could prevent calcium and zinc influx into cells. By the same mechanism, could also prevent L-type calcium channels-mediated heavy metal influx into cells (By similarity). In some cells, could also function as a zinc ion:proton antiporter mediating zinc entry into the lumen of cytoplasmic vesicles. In macrophages, can increase zinc ions concentration into the lumen of cytoplasmic vesicles containing engulfed bacteria and could help inactivate them (PubMed:32441444). Forms a complex with TMC6/EVER1 and TMC8/EVER2 at the ER membrane of keratynocytes which facilitates zinc uptake into the ER (PubMed:18158319). Down-regulates the activity of transcription factors induced by zinc and cytokines (PubMed:18158319).
Alternative names
ZNT1, SLC30A1, Proton-coupled zinc antiporter SLC30A1, Solute carrier family 30 member 1, Zinc transporter 1
Recommended products
Rabbit Polyclonal ZNT1 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC30A1 aa 250-350 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC30A1 aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6M5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ZnT1 also known as SLC30A1 is a critical protein responsible for the export of zinc ions out of cells. This transporter plays an important role in maintaining intracellular zinc homeostasis. The protein has a molecular mass of approximately 52 kDa. ZnT1 is expressed in a wide range of tissues including the brain liver and intestines indicating its significant role in various physiological processes. The protein is located in the cell plasma membrane which aligns with its function of mediating zinc efflux.
- Biological function summary
ZnT1 is essential for regulating the cellular zinc levels and protecting against zinc toxicity. It does not form part of a larger complex; instead it operates as an individual unit to ensure that excess zinc does not accumulate to harmful levels within the cell. By controlling zinc efflux ZnT1 influences processes such as cell signaling and apoptosis which rely on zinc as a cofactor or structural component.
- Pathways
Homeostasis of zinc ions in cells connects ZnT1 to the cellular zinc transport pathway and the metal ion transport pathway. ZnT1 coordinates with intracellular zinc-binding proteins like metallothioneins to modulate zinc availability and transportation within the cell. Proteins such as ZIP4 which facilitate zinc uptake operate in a complementary manner to ZnT1 balancing zinc influx and efflux to maintain cellular function.
- Associated diseases and disorders
Misregulation of ZnT1 has been linked to conditions such as cancer and neurodegenerative diseases. In cancer changes in zinc transport influence tumor growth and progression partly involving interactions with proteins like p53 a critical tumor suppressor modulated by zinc availability. In neurodegenerative diseases abnormal zinc levels can contribute to neuronal damage where ZnT1 interacts with Amyloid Beta plaques affecting the pathology of Alzheimer's disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT1 antibody (ab214356)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue labeling ZnT1 with ab214356 at 1/200 dilution overnight at 4°C followed by a conjugated secondary and DAPI staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT1 antibody (ab214356)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue labeling ZnT1 with ab214356 at 1/500 dilution overnight at 4°C followed by a conjugated secondary and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZnT1 antibody (ab214356)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue labeling ZnT1 with ab214356 at 1/500 dilution overnight at 4°C followed by a conjugated secondary and DAB staining.
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