AB307799

Anti-ZO-1 antibody [HL1185]

Recombinant
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(22 Publications)

Rabbit Recombinant Monoclonal ZO1 tight junction protein antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Dog, Cat, Rat samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TJP1.

View Alternative Names

ZO1, TJP1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] (AB307799)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] (AB307799)

ZO-1 antibody [HL1185] (ab307799) detects ZO-1 protein at cell membrane by immunofluorescent analysis in A341 cells fixed in 4% paraformaldehyde at RT for 15 min. ZO-1 stained by ab307799 diluted at 1/500 (green). Fluoroshield with DAPI (blue)

Supplier Data

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 195 kDa

false

Supplier Data

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

TJP1 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 195 kDa

false

Supplier Data

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Neuro2A, NIH-3T3, Raw264.7 and CSC12 cells (30 μg) were separated by 5%SDS-PAGE,and the membrane was blotted with ZO-1 antibody [HL1185] (ab307799) diluted at 1 : 1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

All lanes:

Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

Lane 1:

Neuro2A at 30 µg

Lane 2:

NIH-3T3 at 30 µg

Lane 3:

Raw264.7 at 30 µg

Lane 4:

CSC12 at 30 µg

Predicted band size: 195 kDa

false

Supplier Data

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 195 kDa

false

Supplier Data

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

Lane 1:

MDCK Whole cell extracts at 30 µg

Lane 2:

PG-4 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 195 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1185

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Dog, Cat, Rat

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TJP1. The exact immunogen used to generate this antibody is proprietary information.

Q07157

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ZO1 tight junction protein also known as TJP1 is a critical component of tight junctions in epithelial and endothelial cells. It is a 225 kDa protein that plays a mechanical role in stabilizing cell-cell junctions by linking the actin cytoskeleton to tight junction membrane proteins. ZO1 is expressed in various tissues including the liver kidney and brain where it is essential for maintaining cell polarity and permeability barriers. It acts as a scaffold facilitating the organization of other tight junction proteins.

Biological function summary

ZO1 tight junction protein maintains cellular barriers and regulates paracellular permeability. This protein forms part of the tight junction complex with other proteins like occludin and claudins contributing to cellular integrity. ZO1's presence at tight junctions helps control the passage of ions and molecules between cells therefore ensuring selective permeability while protecting tissue function. Its ability to interact with multiple proteins enables it to coordinate complex signaling mechanisms that are important for cell adhesion and communication.

Pathways

The tight junction protein ZO1 participates in important signaling pathways like the epithelial barrier formation pathway and the actin cytoskeleton regulation pathway. It interacts with proteins like ZO2 and ZO3 which are critical for enhancing barrier function and cytoskeletal dynamics. By participating in these pathways ZO1 helps mediate both the structural and signaling functions necessary for maintaining tissue homeostasis and responding to cellular stress or injury.

ZO1 protein plays a role in conditions such as inflammatory bowel disease (IBD) and cancer. In IBD the disruption of ZO1 leads to compromised intestinal barriers contributing to disease pathogenesis. The loss or mislocalization of ZO1 has been associated with tumorigenesis in cancers where its interaction with other junctional proteins like claudins could influence cancer cell invasiveness. Understanding ZO1's involvement in these disorders provides insights into potential therapeutic targets for improving epithelial barrier function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed : 7798316, PubMed : 9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed : 21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed : 20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).

See full target information TJP1

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70606 PubMed40916543

2025

Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yao,Bingkai Ren,Qianhui Zhou,Yang Bai,Zhen Feng

Cell death discovery 11:417 PubMed40883266

2025

METTL3-mediated m6A modification of circCDKAL1 regulates macrophage M1 polarization and nasal epithelial cell barrier function in allergic rhinitis through IGF2BP2/JARID2/HMGB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabin Zhan,Dan Luo,Yunlong Fu,Yu Zhou,Rui Li,Xin Wei

Cellular and molecular life sciences : CMLS 82:262 PubMed40580316

2025

NR4A2 attenuates early brain injury after intracerebral hemorrhage by promoting M2 microglial polarization via TLR4/TRAF6/NF-κB pathway inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Di Hu,Cheng Huang,Ling Tang,Jiawen Lei,Jiaqi Wang,Wenzheng Hu,Minshan Chen,Siyuan Song,Lin Lu,Pingyi Xu

Antioxidants (Basel, Switzerland) 14: PubMed40563324

2025

Dynamic Transcriptomic and Cellular Remodeling Underlie Cuprizone-Induced Demyelination and Endogenous Repair in the CNS.

Applications

Unspecified application

Species

Unspecified reactive species

Yantuanjin Ma,Tianyi Liu,Zhipeng Li,Wei Wei,Qiting Zhao,Shufen Wang

Frontiers in physiology 16:1538733 PubMed40529994

2025

Modulation of gut microbiota and immune response by soy peptides mitigates irinotecan induced intestinal toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfa Jing,Dongli Yan

Diabetology & metabolic syndrome 17:182 PubMed40448159

2025

Gamma -aminobutyric acid ameliorates neurological impairments in type 1 diabetes mellitus mice by regulating the "gut flora-LPS-TLR4-NF-ΚB" signalling Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Wang,Lihai Zhang,Xianhe Wang,Jing Dong,Jiaxin Li

BMC medicine 23:285 PubMed40361123

2025

Gut microbiota-derived glutathione from metformin treatment alleviates intestinal ferroptosis induced by ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyan Wang,Xinyu Wang,Chaoyi Wang,Wangxin Yan,Junpeng Xu,Zhengyang Song,Mingli Su,Jingjing Zeng,Qiannian Han,Gaoyi Ruan,Eryao Zhang,Wantie Wang

Glia 73:1565-1588 PubMed40183383

2025

Role of Cx43 and ACKR3 in Modulating Astrocytic Response and Neuronal Survival Post-Subarachnoid Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yan,Shenhao Xie,DianDa Chen,Jinlin Xiao,ErMing Zeng,Tao Hong,Jian Duan

Drug design, development and therapy 19:771-791 PubMed39925879

2025

Metagenomic Sequencing Combined with Metabolomics to Explore Gut Microbiota and Metabolic Changes in Mice with Acute Myocardial Infarction and the Potential Mechanism of Allicin.

Applications

Unspecified application

Species

Unspecified reactive species

Yijie Gao,Gaofeng Qin,Shichao Liang,Jiajie Yin,Baofu Wang,Hong Jiang,Mengru Liu,Fangyuan Luo,Xianlun Li

iScience 27:111517 PubMed39759008

2025

The mesenteric adipokine SFRP5 alleviated intestinal epithelial apoptosis improving barrier dysfunction in Crohn's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng Zhang,Lugen Zuo,Xue Song,Wenjing Zhang,Zi Yang,Zhiyuan Wang,Yibing Guo,Sitang Ge,Lian Wang,Yueyue Wang,Zhijun Geng,Jing Li,Jianguo Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZO-1 antibody [HL1185]

Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] (AB307799)

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

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