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Rabbit Recombinant Monoclonal ZO1 tight junction protein antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Dog, Cat, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TJP1.

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Images

Western blot - Anti-ZO-1 antibody [HL1185] (AB307799), expandable thumbnail
  • Western blot - Anti-ZO-1 antibody [HL1185] (AB307799), expandable thumbnail
  • Western blot - Anti-ZO-1 antibody [HL1185] (AB307799), expandable thumbnail
  • Western blot - Anti-ZO-1 antibody [HL1185] (AB307799), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] (AB307799), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human TJP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q07157

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected
Cat
Tested
Expected
Dog
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Dog
Dilution info
1/1000
Notes

-

Species
Cat
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/1000
Notes

-

Expected
Expected

Species
Mouse, Dog, Cat, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).

Alternative names

ZO1, TJP1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1

Recommended products

Rabbit Recombinant Monoclonal ZO1 tight junction protein antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Dog, Cat, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TJP1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human TJP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q07157
Carrier free
Yes
Clone number
HL1185
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ZO1 tight junction protein also known as TJP1 is a critical component of tight junctions in epithelial and endothelial cells. It is a 225 kDa protein that plays a mechanical role in stabilizing cell-cell junctions by linking the actin cytoskeleton to tight junction membrane proteins. ZO1 is expressed in various tissues including the liver kidney and brain where it is essential for maintaining cell polarity and permeability barriers. It acts as a scaffold facilitating the organization of other tight junction proteins.

Biological function summary

ZO1 tight junction protein maintains cellular barriers and regulates paracellular permeability. This protein forms part of the tight junction complex with other proteins like occludin and claudins contributing to cellular integrity. ZO1's presence at tight junctions helps control the passage of ions and molecules between cells therefore ensuring selective permeability while protecting tissue function. Its ability to interact with multiple proteins enables it to coordinate complex signaling mechanisms that are important for cell adhesion and communication.

Pathways

The tight junction protein ZO1 participates in important signaling pathways like the epithelial barrier formation pathway and the actin cytoskeleton regulation pathway. It interacts with proteins like ZO2 and ZO3 which are critical for enhancing barrier function and cytoskeletal dynamics. By participating in these pathways ZO1 helps mediate both the structural and signaling functions necessary for maintaining tissue homeostasis and responding to cellular stress or injury.

Associated diseases and disorders

ZO1 protein plays a role in conditions such as inflammatory bowel disease (IBD) and cancer. In IBD the disruption of ZO1 leads to compromised intestinal barriers contributing to disease pathogenesis. The loss or mislocalization of ZO1 has been associated with tumorigenesis in cancers where its interaction with other junctional proteins like claudins could influence cancer cell invasiveness. Understanding ZO1's involvement in these disorders provides insights into potential therapeutic targets for improving epithelial barrier function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-ZO-1 antibody [HL1185] (ab307799), expandable thumbnail

    Western blot - Anti-ZO-1 antibody [HL1185] (ab307799)

    Samples were seperated by 5% SDS-PAGE.

    All lanes: Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

    Lane 1: Wild-type (WT) HeLa cell extracts at 30 µg

    Lane 2: TJP1 knockout (KO) HeLa cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 195 kDa

  • Western blot - Anti-ZO-1 antibody [HL1185] (ab307799), expandable thumbnail

    Western blot - Anti-ZO-1 antibody [HL1185] (ab307799)

    Samples were seperated by 5% SDS-PAGE.

    All lanes: Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

    Lane 1: MDCK Whole cell extracts at 30 µg

    Lane 2: PG-4 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 195 kDa

  • Western blot - Anti-ZO-1 antibody [HL1185] (ab307799), expandable thumbnail

    Western blot - Anti-ZO-1 antibody [HL1185] (ab307799)

    Samples were seperated by 5% SDS-PAGE.

    All lanes: Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

    Lane 1: PC-12 whole cell extracts at 30 µg

    Lane 2: Rat2 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 195 kDa

  • Western blot - Anti-ZO-1 antibody [HL1185] (ab307799), expandable thumbnail

    Western blot - Anti-ZO-1 antibody [HL1185] (ab307799)

    Samples were seperated by 5% SDS-PAGE.

    All lanes: Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

    Lane 1: HeLa whole cell extracts at 30 µg

    Lane 2: HepG2 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 195 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] (ab307799), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ZO-1 antibody [HL1185] (ab307799)

    ZO-1 antibody [HL1185] (ab307799) detects ZO-1 protein at cell membrane by immunofluorescent analysis in A341 cells fixed in 4% paraformaldehyde at RT for 15 min. ZO-1 stained by ab307799 diluted at 1/500 (green). Fluoroshield with DAPI (blue)

  • Western blot - Anti-ZO-1 antibody [HL1185] (ab307799), expandable thumbnail

    Western blot - Anti-ZO-1 antibody [HL1185] (ab307799)

    Neuro2A, NIH-3T3, Raw264.7 and CSC12 cells (30 μg) were separated by 5%SDS-PAGE,and the membrane was blotted with
    ZO-1 antibody [HL1185] (ab307799) diluted at 1:1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

    All lanes: Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

    Lane 1: Neuro2A at 30 µg

    Lane 2: NIH-3T3 at 30 µg

    Lane 3: Raw264.7 at 30 µg

    Lane 4: CSC12 at 30 µg

    Predicted band size: 195 kDa

  • Western blot - Anti-ZO-1 antibody [HL1185] (ab307799), expandable thumbnail

    Western blot - Anti-ZO-1 antibody [HL1185] (ab307799)

    HeLa and HepG2 cells (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (ab307799) diluted at 1:1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-ZO-1 antibody [HL1185] (ab307799) at 1/1000 dilution

    Lane 1: HeLa cells at 30 µg

    Lane 2: HepG2 Cells at 30 µg

    Predicted band size: 195 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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