Anti-ZO1 tight junction protein antibody

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p>5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight.</p><p> </p><p>Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052).</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>High background was observed in images.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p>Fix cells in ice-cold MeOH</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p>5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight.</p><p> </p><p>Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052).</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>High background was observed in images.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p>5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight.</p><p> </p><p>Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052).</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }