Anti-ZO1 tight junction protein antibody (ab96587) is a rabbit polyclonal antibody detecting ZO1 tight junction protein in Western Blot, ICC/IF. Suitable for Human, Mouse.
- Over 180 publications
- Trusted since 2010
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- Communications biology 6:12392023Transcranial photobiomodulation improves insulin therapy in diabetic microglial reactivity and the brain drainage system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaojun Liu,Dongyu Li,Tingting Yu,Jingtan Zhu,Oxana Semyachkina-Glushkovskaya,Dan ZhuPubMed 38066234
- Molecular neurobiology 61:1936-19522023Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYipeng Zhao,Chen Chen,Xiuqing Xiao,Ling Fang,Xi Cheng,Yanyu Chang,Fuhua Peng,Jingqi Wang,Shishi Shen,Shilin Wu,Yiying Huang,Wei Cai,Linli Zhou,Wei QiuPubMed 37819429
- International journal of biological sciences 19:4360-43752023Activation of P2Y1R impedes intestinal mucosa repair during colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuchan Huan,Jiao Peng,Yaoyao Chang,Qiansheng Zhang,Tianhang Xing,Danling Jiang,Wenke Chen,Xiangchun Shen,Zhaoxiang Bian,Haitao XiaoPubMed 37781034
- Neurochemical research 48:3610-36242023Compound Dihuang Granule Changes Gut Microbiota of MPTP-Induced Parkinson's Disease Mice via Inhibiting TLR4/NF-κB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhu-Qing He,Peng-Fei Huan,Li Wang,Jian-Cheng HePubMed 37561259
- Nature communications 14:42862023Vγ1 and Vγ4 gamma-delta T cells play opposing roles in the immunopathology of traumatic brain injury in males.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHadi Abou-El-Hassan,Rafael M Rezende,Saef Izzy,Galina Gabriely,Taha Yahya,Bruna K Tatematsu,Karl J Habashy,Juliana R Lopes,Gislane L V de Oliveira,Amir-Hadi Maghzi,Zhuoran Yin,Laura M Cox,Rajesh Krishnan,Oleg Butovsky,Howard L WeinerPubMed 37463881
- Nutrients 15:2023Hydroethanolic Extract of Bulbus Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis by Enhancing Intestinal Barrier Integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmi Lee,You Chul Chung,Kwang-Youn Kim,Chan Ho Jang,Kwang Hoon Song,Youn-Hwan HwangPubMed 37375714
- Chemistry & biodiversity 20:e2023005722023Picroside III, an Active Ingredient of Picrorhiza scrophulariiflora, Ameliorates Experimental Colitis by Protecting Intestinal Barrier Integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuchan Huan,Zhengxian Gao,Peigen Wu,Yingdi Zhang,Haitao Xiao,Jiao PengPubMed 37218365
- Antioxidants (Basel, Switzerland) 12:2023New Indole-3-Propionic Acid and 5-Methoxy-Indole Carboxylic Acid Derived Hydrazone Hybrids as Multifunctional Neuroprotectors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeda Anastassova,Denitsa Stefanova,Nadya Hristova-Avakumova,Irina Georgieva,Magdalena Kondeva-Burdina,Miroslav Rangelov,Nadezhda Todorova,Rumiana Tzoneva,Denitsa YanchevaPubMed 37107353
- Journal of translational medicine 21:2642023Microbiota-derived tryptophan metabolites indole-3-lactic acid is associated with intestinal ischemia/reperfusion injury via positive regulation of YAP and Nrf2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang-Ling Zhang,Xiao-Wei Chen,Yi-Fan Wang,Zhen Hu,Wen-Juan Zhang,Bo-Wei Zhou,Peng-Fei Ci,Ke-Xuan LiuPubMed 37072757
- Biomedicines 11:2023Vunakizumab-IL22, a Novel Fusion Protein, Promotes Intestinal Epithelial Repair and Protects against Gut Injury Induced by the Influenza Virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenchen Shi,Chang Su,Lifeng Cen,Lei Han,Jianguo Tang,Zetian Wang,Xunlong Shi,Dianwen Ju,Yiou Cao,Haiyan ZhuPubMed 37189778
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TJP1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q07157
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Tested | Not recommended | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes 5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight. Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
Species Human | Dilution info 1/500 - 1/3000 | Notes 5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight. Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes High background was observed in images. |
Species Human | Dilution info - | Notes High background was observed in images. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Fix cells in ice-cold MeOH |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).
Alternative names
ZO1, TJP1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1
Recommended products
Anti-ZO1 tight junction protein antibody (ab96587) is a rabbit polyclonal antibody detecting ZO1 tight junction protein in Western Blot, ICC/IF. Suitable for Human, Mouse.
- Over 180 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TJP1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q07157
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ZO1 tight junction protein antibody (ab96587) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in human, mouse samples.
Anti-ZO1 tight junction protein antibody (ab96587) specifically detects ZO1 tight junction protein (UniProt ID: Q07157; Molecular weight: 195kDa) and is sold in 50 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-ZO1 tight junction protein antibody (ab96587) has high sensitivity and specificity.
Anti-ZO1 tight junction protein antibody (ab96587) has been cited over 185 times in peer reviewed journals and is trusted by the scientific community.
Anti-ZO1 tight junction protein antibody (ab96587) has 4 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Western blot - Anti-ZO1 tight junction protein antibody (ab96587)
5% SDS-PAGE
All lanes: Western blot - Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution
All lanes: Mouse testis tissue lysate at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 195 kDa
Western blot - Anti-ZO1 tight junction protein antibody (ab96587)
5% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition:Semi-dry, 18 V, 60min (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: Room temperature for 1 hour.
Washing condition: 5 ml TBST, 4 x 5min.
ECL detection.
All lanes: Western blot - Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 195 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab96587)
Immunofluorescence analysis of ice-cold methanol-fixed (5 min) NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells labeling ZO1 tight junction protein at cell membrane (green) with ab96587 at 1/200 dilution. Blue: Hoechst 33342 staining.
Western blot - Anti-ZO1 tight junction protein antibody (ab96587)
5% SDS-PAGE
All lanes: Western blot - Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Lane 2: C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 195 kDa
Western blot - Anti-ZO1 tight junction protein antibody (ab96587)
5% SDS-PAGE
All lanes: Western blot - Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution
Lane 1: Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 2: ZO1 tight junction protein shRNA-transfected HeLa whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 195 kDa
Western blot - Anti-ZO1 tight junction protein antibody (ab96587)
5% SDS-PAGE
All lanes: Western blot - Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution
All lanes: C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 195 kDa
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