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Goat Polyclonal ZO1 tight junction protein antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Dog, Human samples. Cited in 55 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TJP1 aa 1550 to C-terminus.


Images

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human TJP1 aa 1550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q07157

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Expected
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Dog
Tested
Expected
Expected
Monkey
Predicted
Predicted
Predicted

Tested
Tested

Species

Dog

Dilution info

1/500 - 1/2000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Expected
Expected

Species

Dog

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/250

Notes

-

Expected
Expected

Species

Dog

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Monkey

Dilution info

-

Notes

-

Target data

Function

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity).

Alternative names

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Goat Polyclonal ZO1 tight junction protein antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Dog, Human samples. Cited in 55 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TJP1 aa 1550 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human TJP1 aa 1550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q07157
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

ZO1 tight junction protein maintains cellular barriers and regulates paracellular permeability. This protein forms part of the tight junction complex with other proteins like occludin and claudins contributing to cellular integrity. ZO1's presence at tight junctions helps control the passage of ions and molecules between cells therefore ensuring selective permeability while protecting tissue function. Its ability to interact with multiple proteins enables it to coordinate complex signaling mechanisms that are important for cell adhesion and communication.

Activity summary

The ZO1 tight junction protein also known as TJP1 is a critical component of tight junctions in epithelial and endothelial cells. It is a 225 kDa protein that plays a mechanical role in stabilizing cell-cell junctions by linking the actin cytoskeleton to tight junction membrane proteins. ZO1 is expressed in various tissues including the liver kidney and brain where it is essential for maintaining cell polarity and permeability barriers. It acts as a scaffold facilitating the organization of other tight junction proteins.

Pathways

The tight junction protein ZO1 participates in important signaling pathways like the epithelial barrier formation pathway and the actin cytoskeleton regulation pathway. It interacts with proteins like ZO2 and ZO3 which are critical for enhancing barrier function and cytoskeletal dynamics. By participating in these pathways ZO1 helps mediate both the structural and signaling functions necessary for maintaining tissue homeostasis and responding to cellular stress or injury.

Associated diseases and disorders

ZO1 protein plays a role in conditions such as inflammatory bowel disease (IBD) and cancer. In IBD the disruption of ZO1 leads to compromised intestinal barriers contributing to disease pathogenesis. The loss or mislocalization of ZO1 has been associated with tumorigenesis in cancers where its interaction with other junctional proteins like claudins could influence cancer cell invasiveness. Understanding ZO1's involvement in these disorders provides insights into potential therapeutic targets for improving epithelial barrier function.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ZO1 tight junction protein with ab190085 at 10 μg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085)

    Immunocytochemistry/ Immunofluorescence analysis of primary RPE cells labeling ZO1 tight junction protein with ab190085 at 1/100 dilution.

  • Western blot - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085), expandable thumbnail

    Western blot - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085)

    All lanes: Western blot - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085) at 1/2500 dilution

    All lanes: MDCK cell lysate at 50 µg

    Secondary

    All lanes: Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution

    Predicted band size: 195 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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