Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) is a goat polyclonal antibody detecting ZO1 tight junction protein in Western Blot, IHC-P, ICC/IF. Suitable for Dog, Human.
- Over 50 publications
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- International journal of molecular medicine 52:2023Quercetin alleviates lipopolysaccharide‑induced acute lung injury by inhibiting ferroptosis via the Sirt1/Nrf2/Gpx4 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShihua Deng,Jin Li,Li Li,Sheng Lin,Yueyan Yang,Teng Liu,Ting Zhang,Guangsu Xie,Dongming Wu,Ying XuPubMed 37888753
- Molecular biotechnology 66:2007-20152023Propofol Suppresses LPS-induced BBB Damage by Regulating miR-130a-5p/ZO-1 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Gan,Ying Zhou,Jing Li,Aizhong Wang,Yiyun CaoPubMed 37556107
- Cell communication and signaling : CCS 21:1762023Neutrophil extracellular traps promote angiogenesis in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShifeng Yang,Boshi Sun,Jiacheng Li,Nana Li,Ange Zhang,Xinyu Zhang,Hao Yang,Xiaoming ZouPubMed 37480055
- Chinese medicine 18:852023Gegen-Qinlian decoction alleviates anxiety-like behaviors in methamphetamine-withdrawn mice by regulating Akkermansia and metabolism in the colon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Lu,Yu Fan,Yaqin Peng,Weichao Pan,Demin Du,Xing Xu,Nanqin Li,Teng He,Jiaxun Nie,Pengbo Shi,Feifei Ge,Dekang Liu,Yugen Chen,Xiaowei GuanPubMed 37455317
- Military Medical Research 10:132023Pericytes protect rats and mice from sepsis-induced injuries by maintaining vascular reactivity and barrier function: implication of miRNAs and microvesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZi-Sen Zhang,Yi-Yan Liu,Shuang-Shuang He,Dai-Qin Bao,Hong-Chen Wang,Jie Zhang,Xiao-Yong Peng,Jia-Tao Zang,Yu Zhu,Yue Wu,Qing-Hui Li,Tao Li,Liang-Ming LiuPubMed 36907884
- Nature protocols 18:1584-16202023Generating human blastoids modeling blastocyst-stage embryos and implantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeidar Heidari Khoei,Alok Javali,Harunobu Kagawa,Theresa Maria Sommer,Giovanni Sestini,Laurent David,Jana Slovakova,Maria Novatchkova,Yvonne Scholte Op Reimer,Nicolas RivronPubMed 36792779
- International journal of molecular sciences 24:2023Phillygenin Attenuated Colon Inflammation and Improved Intestinal Mucosal Barrier in DSS-induced Colitis Mice via TLR4/Src Mediated MAPK and NF-κB Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuan-Huan Xue,Jing-Jing Li,Shi-Fei Li,Jing Guo,Rui-Ping Yan,Ting-Gui Chen,Xiang-Hua Shi,Jin-Dong Wang,Li-Wei ZhangPubMed 36768559
- Journal of orthopaedic translation 37:46-602022Fecal microbiota transplantation ameliorates bone loss in mice with ovariectomy-induced osteoporosis via modulating gut microbiota and metabolic function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan-Wei Zhang,Mu-Min Cao,Ying-Juan Li,Pan-Pan Lu,Guang-Chun Dai,Ming Zhang,Hao Wang,Yun-Feng RuiPubMed 36196151
- Fluids and barriers of the CNS 19:732022ROS attenuates TET2-dependent ZO-1 epigenetic expression in cerebral vascular endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Wang,Bei Mao,Keyang Fan,Renqiang Sun,Jialong Zhang,Huazheng Liang,Ying LiuPubMed 36076297
- BioMed research international 2022:87179502022The Protective Effect of Sulodexide on Acute Lung Injury Induced by a Murine Model of Obstructive Jaundice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPenglei Ma,Yue Long,Qiang Fu,Yanhong Liu,Yitian Yang,Weixing Zhao,Qiangwei Liu,Yang Li,Weidong MiPubMed 36060145
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TJP1 aa 1550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q07157
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Dog | Tested | Expected | Expected |
| Monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info 1/500 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).
Alternative names
ZO1, TJP1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1
Recommended products
Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) is a goat polyclonal antibody detecting ZO1 tight junction protein in Western Blot, IHC-P, ICC/IF. Suitable for Dog, Human.
- Over 50 publications
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TJP1 aa 1550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q07157
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) is a goat polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Dog, Human samples.
What is the molecular weight of ZO1 tight junction protein?
Anti-ZO1 tight junction protein - C-terminal (ab190085) specifically detects a band for ZO1 tight junction protein (UniProt: Q07157) at a molecular weight of 195kDa.
Trusted by the scientific community
Anti-ZO1 tight junction protein - C-terminal (ab190085) was first used in a scientific publication in 2014 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-ZO1 tight junction protein - C-terminal (ab190085) has over 25 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ZO1 tight junction protein also known as TJP1 is a critical component of tight junctions in epithelial and endothelial cells. It is a 225 kDa protein that plays a mechanical role in stabilizing cell-cell junctions by linking the actin cytoskeleton to tight junction membrane proteins. ZO1 is expressed in various tissues including the liver kidney and brain where it is essential for maintaining cell polarity and permeability barriers. It acts as a scaffold facilitating the organization of other tight junction proteins.
- Biological function summary
ZO1 tight junction protein maintains cellular barriers and regulates paracellular permeability. This protein forms part of the tight junction complex with other proteins like occludin and claudins contributing to cellular integrity. ZO1's presence at tight junctions helps control the passage of ions and molecules between cells therefore ensuring selective permeability while protecting tissue function. Its ability to interact with multiple proteins enables it to coordinate complex signaling mechanisms that are important for cell adhesion and communication.
- Pathways
The tight junction protein ZO1 participates in important signaling pathways like the epithelial barrier formation pathway and the actin cytoskeleton regulation pathway. It interacts with proteins like ZO2 and ZO3 which are critical for enhancing barrier function and cytoskeletal dynamics. By participating in these pathways ZO1 helps mediate both the structural and signaling functions necessary for maintaining tissue homeostasis and responding to cellular stress or injury.
- Associated diseases and disorders
ZO1 protein plays a role in conditions such as inflammatory bowel disease (IBD) and cancer. In IBD the disruption of ZO1 leads to compromised intestinal barriers contributing to disease pathogenesis. The loss or mislocalization of ZO1 has been associated with tumorigenesis in cancers where its interaction with other junctional proteins like claudins could influence cancer cell invasiveness. Understanding ZO1's involvement in these disorders provides insights into potential therapeutic targets for improving epithelial barrier function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085)
All lanes: Western blot - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) at 1/2500 dilution
All lanes: MDCK cell lysate at 50 µg
SecondaryAll lanes: Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 195 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ZO1 tight junction protein with ab190085 at 10 μg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085)
Immunocytochemistry/ Immunofluorescence analysis of primary RPE cells labeling ZO1 tight junction protein with ab190085 at 1/100 dilution.
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