AB190085

Anti-ZO1 tight junction protein antibody - C-terminal

Name: Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) | Abcam
Brand: Abcam Limited
SKU: AB190085
Price: 505 USD
Availability: InStock
Rating: 4 (28 reviews)

(28 Reviews)

(78 Publications)

Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) is a goat polyclonal antibody detecting ZO1 tight junction protein in Western Blot, IHC-P, ICC/IF. Suitable for Dog, Human.

- Over 50 publications

View Alternative Names

ZO1, TJP1, Tight junction protein 1, Tight junction protein ZO-1, Zona occludens protein 1, Zonula occludens protein 1

3 Images

Supplier Data

Western blot - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085)

All lanes:

Western blot - Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) at 1/2500 dilution

All lanes:

MDCK cell lysate at 50 µg

Secondary

All lanes:

Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution

Predicted band size: 195 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ZO1 tight junction protein with ab190085 at 10 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085)

Immunocytochemistry/ Immunofluorescence analysis of primary RPE cells labeling ZO1 tight junction protein with ab190085 at 1/100 dilution.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Dog, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TJP1 aa 1550-1750. The exact immunogen used to generate this antibody is proprietary information.

Q07157

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-ZO1 tight junction protein antibody - C-terminal (ab190085) is a goat polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Dog, Human samples.

What is the molecular weight of ZO1 tight junction protein?
Anti-ZO1 tight junction protein - C-terminal (ab190085) specifically detects a band for ZO1 tight junction protein (UniProt: Q07157) at a molecular weight of 195kDa.

Trusted by the scientific community
Anti-ZO1 tight junction protein - C-terminal (ab190085) was first used in a scientific publication in 2014 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-ZO1 tight junction protein - C-terminal (ab190085) has over 25 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ZO1 tight junction protein also known as TJP1 is a critical component of tight junctions in epithelial and endothelial cells. It is a 225 kDa protein that plays a mechanical role in stabilizing cell-cell junctions by linking the actin cytoskeleton to tight junction membrane proteins. ZO1 is expressed in various tissues including the liver kidney and brain where it is essential for maintaining cell polarity and permeability barriers. It acts as a scaffold facilitating the organization of other tight junction proteins.

Biological function summary

ZO1 tight junction protein maintains cellular barriers and regulates paracellular permeability. This protein forms part of the tight junction complex with other proteins like occludin and claudins contributing to cellular integrity. ZO1's presence at tight junctions helps control the passage of ions and molecules between cells therefore ensuring selective permeability while protecting tissue function. Its ability to interact with multiple proteins enables it to coordinate complex signaling mechanisms that are important for cell adhesion and communication.

Pathways

The tight junction protein ZO1 participates in important signaling pathways like the epithelial barrier formation pathway and the actin cytoskeleton regulation pathway. It interacts with proteins like ZO2 and ZO3 which are critical for enhancing barrier function and cytoskeletal dynamics. By participating in these pathways ZO1 helps mediate both the structural and signaling functions necessary for maintaining tissue homeostasis and responding to cellular stress or injury.

ZO1 protein plays a role in conditions such as inflammatory bowel disease (IBD) and cancer. In IBD the disruption of ZO1 leads to compromised intestinal barriers contributing to disease pathogenesis. The loss or mislocalization of ZO1 has been associated with tumorigenesis in cancers where its interaction with other junctional proteins like claudins could influence cancer cell invasiveness. Understanding ZO1's involvement in these disorders provides insights into potential therapeutic targets for improving epithelial barrier function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed : 7798316, PubMed : 9792688). Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed : 21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed : 20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).

See full target information TJP1

style

left-column

Publications (78)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 16:1658389 PubMed40964620

2025

Blood-spinal cord barrier alterations in a mouse model of centrifugation-induced hypergravity.

Applications

Unspecified application

Species

Unspecified reactive species

David Dubayle,Jean-Luc Morel,Sighild Lemarchant

Journal of inflammation research 18:6263-6280 PubMed40391232

2025

Si-Ni Decoction as a Potential Treatment for Ulcerative Colitis: Modulation of Gut Microbiota and AKT1 Inhibition Through Network Pharmacology and in vivo Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Lihao Shi,Leilei Chen,Guiyuan Jin,Yonghong Yang,Fengqin Zhu,Guangxi Zhou

Biological & pharmaceutical bulletin 48:577-594 PubMed40350306

2025

Nephropathy II Decoction Attenuates Renal Fibrosis via Regulating TLR4 and Gut Microbiota Along the Gut-Kidney Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Liu,Yujiu Gao,Yirui Chen,Liting Zhu,Fu Rao,Yuhan Huang,Yini Zeng,Rui Cai,Fangyan Wang,Jinguo Cheng

Pharmaceutics 17: PubMed40284517

2025

In Vitro Toxicity of Cetalkonium Chloride on Corneal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joo-Hee Park,Choul Yong Park

Scientific reports 15:9883 PubMed40121325

2025

Establishment and validation of red fox (vulpes vulpes) airway epithelial cell cultures at the air-liquid-interface.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas W Oehm,Blandina I Oliveira Esteves,Udo Hetzel,Marco P Alves,Manuela Schnyder

Journal of pharmaceutical analysis 15:101042 PubMed39902458

2025

Phillygenin ameliorates tight junction proteins reduction, fibrosis, and apoptosis in mice with chronic colitis via TGR5-mediated PERK-eIF2α-Ca pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huanhuan Xue,Peijie Li,Jing Guo,Tinggui Chen,Shifei Li,Liwei Zhang

Autophagy 21:619-638 PubMed39477683

2024

Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α.

Applications

Unspecified application

Species

Unspecified reactive species

Qinyu Li,Kai Zeng,Qian Chen,Chenglin Han,Xi Wang,Beining Li,Jianping Miao,Bolong Zheng,Jihong Liu,Xianglin Yuan,Bo Liu

Fluids and barriers of the CNS 21:76 PubMed39334382

2024

NX210c drug candidate peptide strengthens mouse and human blood-brain barriers.

Applications

Unspecified application

Species

Unspecified reactive species

Chris Greene,Nicolas Rebergue,Gwen Fewell,Damir Janigro,Yann Godfrin,Matthew Campbell,Sighild Lemarchant

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 28:239-252 PubMed38682172

2024

Dexmedetomidine alleviates blood-brain barrier disruption in rats after cerebral ischemia-reperfusion by suppressing JNK and p38 MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Canmin Zhu,Dili Wang,Chang Chang,Aofei Liu,Ji Zhou,Ting Yang,Yuanfeng Jiang,Xia Li,Weijian Jiang

Drug delivery and translational research 14:2629-2641 PubMed38498080

2024

RGD-coated polymeric microbubbles promote ultrasound-mediated drug delivery in an inflamed endothelium-pericyte co-culture model of the blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Hark,Junlin Chen,Julia Blöck,Eva Miriam Buhl,Harald Radermacher,Robert Pola,Michal Pechar,Tomáš Etrych,Quim Peña,Anne Rix,Natascha I Drude,Fabian Kiessling,Twan Lammers,Jan-Niklas May

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ZO1 tight junction protein antibody - C-terminal

Western blot - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085)

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody - C-terminal (AB190085)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (78)

Product promise