Rabbit Recombinant Monoclonal ZO1 tight junction protein antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8037.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48972.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65976.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55342.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25174.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (AB251567)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).
Alternative names
ZO1, TJP1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1
Recommended products
Rabbit Recombinant Monoclonal ZO1 tight junction protein antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19945-224
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251567 is the carrier-free version of Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ZO1 tight junction protein also known as TJP1 is a critical component of tight junctions in epithelial and endothelial cells. It is a 225 kDa protein that plays a mechanical role in stabilizing cell-cell junctions by linking the actin cytoskeleton to tight junction membrane proteins. ZO1 is expressed in various tissues including the liver kidney and brain where it is essential for maintaining cell polarity and permeability barriers. It acts as a scaffold facilitating the organization of other tight junction proteins.
- Biological function summary
ZO1 tight junction protein maintains cellular barriers and regulates paracellular permeability. This protein forms part of the tight junction complex with other proteins like occludin and claudins contributing to cellular integrity. ZO1's presence at tight junctions helps control the passage of ions and molecules between cells therefore ensuring selective permeability while protecting tissue function. Its ability to interact with multiple proteins enables it to coordinate complex signaling mechanisms that are important for cell adhesion and communication.
- Pathways
The tight junction protein ZO1 participates in important signaling pathways like the epithelial barrier formation pathway and the actin cytoskeleton regulation pathway. It interacts with proteins like ZO2 and ZO3 which are critical for enhancing barrier function and cytoskeletal dynamics. By participating in these pathways ZO1 helps mediate both the structural and signaling functions necessary for maintaining tissue homeostasis and responding to cellular stress or injury.
- Associated diseases and disorders
ZO1 protein plays a role in conditions such as inflammatory bowel disease (IBD) and cancer. In IBD the disruption of ZO1 leads to compromised intestinal barriers contributing to disease pathogenesis. The loss or mislocalization of ZO1 has been associated with tumorigenesis in cancers where its interaction with other junctional proteins like claudins could influence cancer cell invasiveness. Understanding ZO1's involvement in these disorders provides insights into potential therapeutic targets for improving epithelial barrier function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8037.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)
This data was developed using Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human bronchial epithelium tissue labeling ZO1 tight junction protein with Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining in epithelial and endothelial cells of human bronchial epithelium (PMID: 17585317) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48972.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)
This data was developed using Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ZO1 tight junction protein with Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in glomerulus of human kidney (PMID: 18618131) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65976.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)
This data was developed using Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ZO1 tight junction protein with Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in breast cancer (PMID: 9846967) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img55342.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)
This data was developed using Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ZO1 tight junction protein with Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in glomerular of mouse kidney (PMID: 18618131) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25174.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)
This data was developed using Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ZO1 tight junction protein with Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic in endothelial cells of rat spleen (PMID: 17585317) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86358.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)
This data was developed using Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546, the same antibody clone in a different buffer formulation.Negative control - Immunohistochemical analysis of paraffin-embedded human liver tissue labeling ZO1 tight junction protein with Anti-ZO1 tight junction protein antibody [EPR19945-224] ab221546 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative staining in lymphocytes of human tonsil (PMID: 17585317) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-224-bsa-and-azide-free-ab251567--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86358.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-224] - BSA and Azide free (ab251567)")
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