Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) is a rabbit monoclonal antibody detecting ZO1 tight junction protein in Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41359.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)")
![Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunocytochemistry-immunofluorescence-img22913.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)")
![Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunocytochemistry-immunofluorescence-img73151.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)")
![Flow Cytometry (Intracellular) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--flow-cytometry-intracellular-img13660.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65578.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)")
Publications
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- Journal of translational medicine 21:9042023C3a/C3aR synergies with TGF-β to promote epithelial-mesenchymal transition of renal tubular epithelial cells via the activation of the NLRP3 inflammasome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanyu You,Kun Nie,Xiaoting Wu,Mengjie Weng,Liyan Yang,Yi Chen,Jiong Cui,Jianxin WanPubMed 38082306
- Aging and disease 14:2193-22142023Healthy Human Fecal Microbiota Transplantation into Mice Attenuates MPTP-Induced Neurotoxicity via AMPK/SOD2 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenchao Xie,Mahui Zhang,Yuqi Luo,Dana Jin,Xingfang Guo,Wanlin Yang,Jialing Zheng,Hongfei Zhang,Lu Zhang,Chao Deng,Wenhua Zheng,Eng-King Tan,Kunlin Jin,Shuzhen Zhu,Qing WangPubMed 37199590
- Nature communications 14:77402023Parabacteroides distasonis ameliorates insulin resistance via activation of intestinal GPR109a.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYonggan Sun,Qixing Nie,Shanshan Zhang,Huijun He,Sheng Zuo,Chunhua Chen,Jingrui Yang,Haihong Chen,Jielun Hu,Song Li,Jiaobo Cheng,Baojie Zhang,Zhitian Zheng,Shijie Pan,Ping Huang,Lu Lian,Shaoping NiePubMed 38007572
- Microbiome 11:2482023Surgery-induced gut microbial dysbiosis promotes cognitive impairment via regulation of intestinal function and the metabolite palmitic amide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCailong Pan,Huiwen Zhang,Lingyuan Zhang,Lu Chen,Lu Xu,Ning Xu,Xue Liu,Qinghai Meng,Xiaoliang Wang,Zhi-Yuan ZhangPubMed 37936242
- Antioxidants (Basel, Switzerland) 12:2023Endothelial Notch Signaling Regulates the Function of the Retinal Pigment Epithelial Barrier via EC Angiocrine Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYali Niu,Yixuan Xi,Yutong Jing,Ziyi Zhou,Xiaojia Sun,Guoheng Zhang,Tianhao Yuan,Tianfang Chang,Guorui DouPubMed 38001832
- iScience 26:1083922023relieves intrahepatic cholestasis of pregnancy through bile acid/FXR-FGF15 in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanxiang Sun,Xiujuan Su,Yang Liu,Guohua Li,Qiaoling DuPubMed 38025767
- International journal of molecular sciences 24:2023Efficacy of Mesenchymal-Stromal-Cell-Derived Extracellular Vesicles in Ameliorating Cisplatin Nephrotoxicity, as Modeled Using Three-Dimensional, Gravity-Driven, Two-Layer Tubule-on-a-Chip (3D-MOTIVE Chip).Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Jeong Kwon,Seong-Hye Hwang,Seungwan Seo,Jaesung Park,Seokwoo Park,Sejoong KimPubMed 37958709
- Experimental and therapeutic medicine 26:5792023Hyperforin modulates MAPK/CCL11 signaling to reduce the inflammatory response of nasal mucosal epithelial cells caused by allergic rhinitis by targeting BCL6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Xu,Wen SuPubMed 38023351
- Nutrients 15:2023Ketogenic Diet Exacerbates L-Arginine-Induced Acute Pancreatitis and Reveals the Therapeutic Potential of Butyrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHe Xia,Jing Guo,Jian Shen,Shiman Jiang,Shengyi Han,Lanjuan LiPubMed 37892502
- Cellular & molecular immunology 20:1379-13922023A de novo missense mutation in MPP2 confers an increased risk of Vogt-Koyanagi-Harada disease as shown by trio-based whole-exome sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianyang Liu,Jiayu Meng,Xingyun Liao,Yusen Liu,Qian Zhou,Zongren Xu,Shuming Yin,Qingfeng Cao,Guannan Su,Siyuan He,Wanqian Li,Xiaotang Wang,Guoqing Wang,Dali Li,Peizeng Yang,Shengping HouPubMed 37828081
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-Fr | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Expected | Not recommended | Expected | Tested |
| Rat | Not recommended | Not recommended | Expected | Not recommended | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/60 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).
Alternative names
ZO1, TJP1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1
Recommended products
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) is a rabbit monoclonal antibody detecting ZO1 tight junction protein in Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19945-296
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P in human, mouse, rat samples.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) specifically detects ZO1 tight junction protein (UniProt ID: Q07157; Molecular weight: 195kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has been cited over 75 times in peer reviewed journals and is trusted by the scientific community.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has 6 independent reviews from customers.
Related Products
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41359.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ZO1 tight junction protein with ab221547 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in vascular endothelial cells and glomerular of human kidney (PMID: 18618131) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunocytochemistry-immunofluorescence-img22913.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling ZO1 tight junction protein with ab221547 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.![Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunocytochemistry-immunofluorescence-img73151.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (human epithelial cell line from embryonic kidney) cells labeling ZO1 tight junction protein with ab221547 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in HEK-293 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.![Flow Cytometry (Intracellular) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--flow-cytometry-intracellular-img13660.jpg "Flow Cytometry (Intracellular) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
Flow Cytometry (Intracellular) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling ZO1 tight junction protein with ab221547 at 1/60 dilution (red) compared with an Isotype control details (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65578.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ZO1 tight junction protein with ab221547 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining in endothelial cells of rat spleen (PMID: 17585317) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109412.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ZO1 tight junction protein with ab221547 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in glomerular of mouse kidney (PMID:17585317; PMID: 18618131) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84045.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ZO1 tight junction protein with ab221547 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in breast cancer (PMID: 9846967) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109412.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547), expandable thumbnail](https://content.abcam.com/products/images/zo1-tight-junction-protein-antibody-epr19945-296-ab221547--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84045.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547)")
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