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AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

5

(6 Reviews)

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(187 Publications)

Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) is a rabbit monoclonal antibody detecting ZO1 tight junction protein in Flow Cytometry (Intra), IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications

View Alternative Names

ZO1, TJP1, Tight junction protein 1, Tight junction protein ZO-1, Zona occludens protein 1, Zonula occludens protein 1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ZO1 tight junction protein with ab221547 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in vascular endothelial cells and glomerular of human kidney (PMID : 18618131) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling ZO1 tight junction protein with ab221547 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red).

Secondary antibody only control : Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling ZO1 tight junction protein with ab221547 at 1/60 dilution (red) compared with an Isotype control details (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HEK-293 (human epithelial cell line from embryonic kidney) cells labeling ZO1 tight junction protein with ab221547 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in HEK-293 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red).

Secondary antibody only control : Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ZO1 tight junction protein with ab221547 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in breast cancer (PMID : 9846967) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ZO1 tight junction protein with ab221547 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining in endothelial cells of rat spleen (PMID : 17585317) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ZO1 tight junction protein with ab221547 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic in glomerular of mouse kidney (PMID : 17585317; PMID : 18618131) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

  • Carrier free

    Anti-ZO1 tight junction protein antibody [EPR19945-296] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19945-296

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Product Specifications
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P in human, mouse, rat samples.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) specifically detects ZO1 tight junction protein (UniProt ID: Q07157; Molecular weight: 195kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has been cited over 75 times in peer reviewed journals and is trusted by the scientific community.
Anti-ZO1 tight junction protein antibody [EPR19945-296] (ab221547) has 6 independent reviews from customers.

Related Products

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed : 7798316, PubMed : 9792688). Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed : 21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed : 20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).
See full target information TJP1
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Publications (187)

Recent publications for all applications. Explore the full list and refine your search

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Quercetin promotes angiogenesis and protects the blood-spinal cord barrier structure after spinal cord injury by targeting the PI3K/Akt signaling pathway.

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Journal of nanobiotechnology 23:581 PubMed40847355

2025

Ultrasound molecular imaging of M2 macrophages for early detection of chronic rejection in heart transplantation.

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Jia Xu,Cheng Deng,Tang Gao,Mengrong He,Wenpei Fu,Xin Zhang,Yin Bai,Jiani Qiu,Rui Wang,Yihan Chen,Qiaofeng Jin,Li Zhang,Qing Lv,Mingxing Xie,Wenqian Wu

Frontiers in pharmacology 16:1500157 PubMed40822467

2025

Shenfu injection promotes self-renewal of intestinal stem cells in sepsis-induced intestinal injury via inducing ATF4 expression.

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Yunxin Deng,Lingyun Lu,Jinrong Li,Liya Gao,Yihui Wang,Nataša Petrović,Fengyong Yang,You Li,Mei Meng

International journal of molecular sciences 26: PubMed40806313

2025

Combined Pharmacological Conditioning of Endothelial Cells for Improved Vascular Graft Endothelialization.

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Zhiyao Lu,Xuqian Zhou,Xiaowen Liu,Chunyan Liu,Junfeng Zhang,Lei Dong

Scientific reports 15:27159 PubMed40715435

2025

Gentiopicroside improves DSS-induced ulcerative colitis and secondary liver injury in mice by enhancing the intestinal barrier and regulating the gut microbiome.

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Liu Cao,Mengyuan Niu,Xiaoqing Tang,Yiting Wang,Xianjing Hu,Weibo Dai

Bioactive materials 52:753-772 PubMed40688064

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Modular assembled biomimetic nanobubbles for synergistic therapy of ischemic stroke cascade modulation thrombo-inflammatory network.

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Mingxi Li,Xuan Xu,Guoyan Tang,Huan Wu,Yakun Wang,Chen Cheng,Hongde Liu,Fang Yang

Materials today. Bio 33:102036 PubMed40688662

2025

Curcumin-loaded milk-derived sEVs fused with platelet membrane attenuate endothelial senescence and promote spinal cord injury recovery in diabetic mice.

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Yaozhi He,Siyuan Zou,Jiawei Wang,Wenbin Zhang,Sheng Lu,Mengxian Jia,Yumin Wu,Xiaowu Lin,Ziwei Fan,Qishun Liang,Yizhe Sheng,Qichuan Zhuge,Bi Chen,Minyu Zhu,Honglin Teng

Translational psychiatry 15:239 PubMed40645949

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Integrative analysis identifies IL-6/JUN/MMP-9 pathway destroyed blood-brain-barrier in autism mice via machine learning and bioinformatic analysis.

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Cong Hu,Heli Li,Jinru Cui,Yunjie Li,Feiyan Zhang,Hao Li,Xiaoping Luo,Yan Hao

Frontiers in bioengineering and biotechnology 13:1570270 PubMed40635690

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Development and application of a stepwise-assembled modular biomimetic lung chip for analyzing formaldehyde-induced cellular ferroptosis.

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Siyu Chen,Zezhi Li,Quanping Yan,Chenfeng Hua,Pingping Shang,Kejian Liu,Junwei Zhao,Guangxiang Jin,Xiang Li,Fuwei Xie

Scientific reports 15:20985 PubMed40596186

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Deficiency of integrin β4 contributes bronchopulmonary dysplasia by compromising cellular stability through the activation of RhoA-(ZO-1) signaling pathways.

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Yinxiu Chi,Xianhui Wang,Dongliang Zhang,Jingjing Han,Xiaoyun Shao,Yang Xiang,Linhong Deng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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