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AB322718

Anti-ZO1 tight junction protein antibody [EPR26602-80] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal ZO1 tight junction protein antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

ZO1, TJP1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1

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Sandwich ELISA - Anti-ZO1 tight junction protein antibody [EPR26602-80] - BSA and Azide free (Detector) (AB322718)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ZO1 tight junction protein antibody [EPR26602-80] - BSA and Azide free (Detector) (AB322718)

Sandwich ELISA of ab322716 with the capture antibody ab322717 dilution at 2 ug/mL and detector antibody ab322718 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-ZO1 tight junction protein antibody [EPR26602-80] - BSA and Azide free (Detector) (AB322718)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ZO1 tight junction protein antibody [EPR26602-80] - BSA and Azide free (Detector) (AB322718)

Interpolated concentration of native ZO1 tight junction protein was measured at different sample concentrations. Undiluted samples are as follows : mouse brain tissue extract (500 ug/mL) and rat brain tissue extract (500 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-ZO1 tight junction protein antibody [EPR26602-80] - BSA and Azide free (Detector) (AB322718)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ZO1 tight junction protein antibody [EPR26602-80] - BSA and Azide free (Detector) (AB322718)

Interpolated concentration of native ZO1 tight junction protein was measured at different sample concentrations. Undiluted samples are as follows : HT-29 cell extract (100 ug/mL), MCF-7 cell extract (400 ug/mL) and Hela cell extract (200 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26602-80

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab322718 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Mouse/Rat ZO1 tight junction protein. The recommended pair for sandwich ELISA is:

Capture: ab322717, Human/Mouse/Rat ZO1 tight junction protein Capture Antibody (unconjugated)

Detector: ab322718, Human/Mouse/Rat ZO1 tight junction protein Detector Antibody (unconjugated)

The reference range value is 78.13-5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed : 7798316, PubMed : 9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed : 21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed : 20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity).
See full target information TJP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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