Rabbit Polyclonal Zonula occludens protein 3/ZO3 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Tjp3 aa 850 to C-terminus.
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Publications
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- Disease markers 2022:18393412022LTB4 Promotes Acute Lung Injury via Upregulating the PLC-1/TLR4/NF-B Pathway in One-Lung Ventilation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Luo,Qingjie Ma,Heng Tang,Xi Zou,Xin Guo,Yuzhen Hu,Kejiang Zhou,Rui LiuPubMed 35059042
- Nutrients 13:2021Qualitative Nitrogen Malnutrition Damages Gut and Alters Microbiome in Adult Mice. A Preliminary Histopathological Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanni Corsetti,Claudia Romano,Evasio Pasini,Cristian Testa,Francesco S DioguardiPubMed 33810512
- Oncology reports 45:65-722021Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihua Wang,Nv Mu,Nina QuPubMed 33416135
- BMC complementary medicine and therapies 20:3742020Acorus tatarinowii Schott extract reduces cerebral edema caused by ischemia-reperfusion injury in rats: involvement in regulation of astrocytic NKCC1/AQP4 and JNK/iNOS-mediated signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Chen Lee et. al.PubMed 33298024
- Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 47:211-2192017Epithelial disruptions, but not immune cell invasion, induced secretory dysfunction following innate immune activation in a novel model of acute salivary gland injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbeer Shaalan,Guy Carpenter,Gordon ProctorPubMed 29160910
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Tjp3 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QXY1
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Mouse | Tested | Expected |
| Rat | Tested | Tested |
| Dog | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
Tjp1, Tjp2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:17000770). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PatJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (By similarity). Promotes cell-cycle progression through the sequestration of cyclin D1 (Ccnd1) at tight junctions during mitosis which prevents Ccnd1 degradation during M-phase and enables S-phase transition (PubMed:21411630). With Tjp1 and Tjp2, participates in the junctional retention and stability of the transcription factor DbpA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to Tjp2, Tjp3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (PubMed:17000770, PubMed:18172007).
Alternative names
Zo3, Tjp3, Tight junction protein ZO-3, Tight junction protein 3, Zona occludens protein 3, Zonula occludens protein 3
Recommended products
Rabbit Polyclonal Zonula occludens protein 3/ZO3 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Tjp3 aa 850 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Tjp3 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QXY1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zonula occludens protein 3 (ZO-3) also known as Tight junction protein 3 is a component of tight junction complexes located in epithelial and endothelial cells. ZO-3 exhibits a molecular mass of approximately 130 kDa. Within the cellular environment ZO-3 serves as a scaffold protein anchoring transmembrane proteins to the actin cytoskeleton maintaining tight junction integrity. Expressions of ZO-3 occur in tissues where tight junctions are present including the intestinal epithelium and blood-brain barrier.
- Biological function summary
ZO-3 participates with other proteins in maintaining cellular polarity and regulating paracellular permeability. It forms a part of the tight junction complex where it interacts with proteins like ZO-1 and occludin. Through these interactions ZO-3 contributes to the junctional barrier between adjacent cells playing an essential role in controlling the passage of molecules and ions through these cellular layers. ZO-3 also helps in signal transduction pathways linked to cellular proliferation and differentiation.
- Pathways
ZO-3 plays a significant role in tight junction signaling and the establishment of epithelial cell adhesion. Within these pathways ZO-3 collaborates with ZO-1 and other junctional proteins forming a network that regulates cellular responses to extracellular stimuli. These associations reinforce the epithelial barrier and help manage processes such as inflammation and tissue repair important for maintaining tissue homeostasis.
- Associated diseases and disorders
Zonula occludens protein 3 has a connection to conditions like inflammatory bowel disease (IBD) and certain cancers. Variations or disruptions in ZO-3 expression can weaken the intestinal barrier having potentially contributing effects on the development of IBD. Furthermore ZO-3 has an association with tumor suppressor proteins like ZO-1 suggesting that its dysfunction might play a role in the progression of cancerous growths by altering barrier properties and influencing cell proliferation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143)
All lanes: Western blot - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143) at 0.5 µg/mL
Lane 1: Rat brain tissue lysate
Lane 2: Rat heart tissue lysate
Predicted band size: 103 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143)
Immunohistochemical analysis of Paraffin-embedded Mouse liver tissue labeling Zonula occludens protein 3/ZO3 with ab191143 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143)
Immunohistochemical analysis of Paraffin-embedded Rat intestine tissue labeling Zonula occludens protein 3/ZO3 with ab191143 at 1 μg/ml.
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