AB191143

Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal

Name: Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143) | Abcam
Brand: Abcam Limited
SKU: AB191143
Rating: 4 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal Zonula occludens protein 3/ZO3 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse Tjp3 aa 850 to C-terminus.

View Alternative Names

Zo3, Tjp3, Tight junction protein ZO-3, Tight junction protein 3, Zona occludens protein 3, Zonula occludens protein 3

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (AB191143)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (AB191143)

Immunohistochemical analysis of Paraffin-embedded Rat intestine tissue labeling Zonula occludens protein 3/ZO3 with ab191143 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (AB191143)

Immunohistochemical analysis of Paraffin-embedded Mouse liver tissue labeling Zonula occludens protein 3/ZO3 with ab191143 at 1 μg/ml.

Supplier Data

Western blot - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (AB191143)

All lanes:

Western blot - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (ab191143) at 0.5 µg/mL

Lane 1:

Rat brain tissue lysate

Lane 2:

Rat heart tissue lysate

Predicted band size: 103 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Tjp3 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9QXY1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Zonula occludens protein 3 (ZO-3) also known as Tight junction protein 3 is a component of tight junction complexes located in epithelial and endothelial cells. ZO-3 exhibits a molecular mass of approximately 130 kDa. Within the cellular environment ZO-3 serves as a scaffold protein anchoring transmembrane proteins to the actin cytoskeleton maintaining tight junction integrity. Expressions of ZO-3 occur in tissues where tight junctions are present including the intestinal epithelium and blood-brain barrier.

Biological function summary

ZO-3 participates with other proteins in maintaining cellular polarity and regulating paracellular permeability. It forms a part of the tight junction complex where it interacts with proteins like ZO-1 and occludin. Through these interactions ZO-3 contributes to the junctional barrier between adjacent cells playing an essential role in controlling the passage of molecules and ions through these cellular layers. ZO-3 also helps in signal transduction pathways linked to cellular proliferation and differentiation.

Pathways

ZO-3 plays a significant role in tight junction signaling and the establishment of epithelial cell adhesion. Within these pathways ZO-3 collaborates with ZO-1 and other junctional proteins forming a network that regulates cellular responses to extracellular stimuli. These associations reinforce the epithelial barrier and help manage processes such as inflammation and tissue repair important for maintaining tissue homeostasis.

Zonula occludens protein 3 has a connection to conditions like inflammatory bowel disease (IBD) and certain cancers. Variations or disruptions in ZO-3 expression can weaken the intestinal barrier having potentially contributing effects on the development of IBD. Furthermore ZO-3 has an association with tumor suppressor proteins like ZO-1 suggesting that its dysfunction might play a role in the progression of cancerous growths by altering barrier properties and influencing cell proliferation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tjp1, Tjp2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed : 17000770). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PatJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (By similarity). Promotes cell-cycle progression through the sequestration of cyclin D1 (Ccnd1) at tight junctions during mitosis which prevents Ccnd1 degradation during M-phase and enables S-phase transition (PubMed : 21411630). With Tjp1 and Tjp2, participates in the junctional retention and stability of the transcription factor DbpA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to Tjp2, Tjp3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (PubMed : 17000770, PubMed : 18172007).

See full target information Tjp3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39480513

2024

Flavin-containing monooxygenase 2 confers cardioprotection in ischemia models through its disulfide bond catalytic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Qingnian Liu,Jiniu Huang,Hao Ding,Yue Tao,Jinliang Nan,Changchen Xiao,Yingchao Wang,Rongrong Wu,Cheng Ni,Zhiwei Zhong,Wei Zhu,Jinghai Chen,Chenyun Zhang,Xiao He,Danyang Xiong,Xinyang Hu,Jian'an Wang

Disease markers 2022:1839341 PubMed35059042

2022

LTB4 Promotes Acute Lung Injury via Upregulating the PLC-1/TLR4/NF-B Pathway in One-Lung Ventilation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Luo,Qingjie Ma,Heng Tang,Xi Zou,Xin Guo,Yuzhen Hu,Kejiang Zhou,Rui Liu

Nutrients 13: PubMed33810512

2021

Qualitative Nitrogen Malnutrition Damages Gut and Alters Microbiome in Adult Mice. A Preliminary Histopathological Study.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Corsetti,Claudia Romano,Evasio Pasini,Cristian Testa,Francesco S Dioguardi

Oncology reports 45:65-72 PubMed33416135

2021

Methylation of the miR‑29b‑3p promoter contributes to angiogenesis, invasion, and migration in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Wang,Nv Mu,Nina Qu

BMC complementary medicine and therapies 20:374 PubMed33298024

2020

Acorus tatarinowii Schott extract reduces cerebral edema caused by ischemia-reperfusion injury in rats: involvement in regulation of astrocytic NKCC1/AQP4 and JNK/iNOS-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chen Lee,Shung-Te Kao,Chin-Yi Cheng

Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 47:211-219 PubMed29160910

2017

Epithelial disruptions, but not immune cell invasion, induced secretory dysfunction following innate immune activation in a novel model of acute salivary gland injury.

Applications

Unspecified application

Species

Unspecified reactive species

Abeer Shaalan,Guy Carpenter,Gordon Proctor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (AB191143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (AB191143)

Western blot - Anti-Zonula occludens protein 3/ZO3 antibody - C-terminal (AB191143)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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