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Rabbit Recombinant Monoclonal Zonula occludens protein 3/ZO3 antibody. Plasma Membrane marker. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (AB181991), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (AB181991), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/100000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:16129888). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (PubMed:16129888). Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition (PubMed:21411630). With TJP1 and TJP2, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to TJP2, TJP3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (By similarity).

Alternative names

ZO3, TJP3, Tight junction protein ZO-3, Tight junction protein 3, Zona occludens protein 3, Zonula occludens protein 3

Recommended products

Rabbit Recombinant Monoclonal Zonula occludens protein 3/ZO3 antibody. Plasma Membrane marker. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13414
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Zonula occludens protein 3 (ZO-3) also known as Tight junction protein 3 is a component of tight junction complexes located in epithelial and endothelial cells. ZO-3 exhibits a molecular mass of approximately 130 kDa. Within the cellular environment ZO-3 serves as a scaffold protein anchoring transmembrane proteins to the actin cytoskeleton maintaining tight junction integrity. Expressions of ZO-3 occur in tissues where tight junctions are present including the intestinal epithelium and blood-brain barrier.

Biological function summary

ZO-3 participates with other proteins in maintaining cellular polarity and regulating paracellular permeability. It forms a part of the tight junction complex where it interacts with proteins like ZO-1 and occludin. Through these interactions ZO-3 contributes to the junctional barrier between adjacent cells playing an essential role in controlling the passage of molecules and ions through these cellular layers. ZO-3 also helps in signal transduction pathways linked to cellular proliferation and differentiation.

Pathways

ZO-3 plays a significant role in tight junction signaling and the establishment of epithelial cell adhesion. Within these pathways ZO-3 collaborates with ZO-1 and other junctional proteins forming a network that regulates cellular responses to extracellular stimuli. These associations reinforce the epithelial barrier and help manage processes such as inflammation and tissue repair important for maintaining tissue homeostasis.

Associated diseases and disorders

Zonula occludens protein 3 has a connection to conditions like inflammatory bowel disease (IBD) and certain cancers. Variations or disruptions in ZO-3 expression can weaken the intestinal barrier having potentially contributing effects on the development of IBD. Furthermore ZO-3 has an association with tumor suppressor proteins like ZO-1 suggesting that its dysfunction might play a role in the progression of cancerous growths by altering barrier properties and influencing cell proliferation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991), expandable thumbnail

    Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)

    All lanes: Western blot - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991) at 1/100000 dilution

    Lane 1: Caco2 cell lysate at 20 µg

    Lane 2: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 103 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Zonula occludens protein 3/ZO3 antibody [EPR13414] - Plasma Membrane Marker (ab181991)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cell line staining Zonula occludens protein 3 using ab181991 at a 1/250 dilution (green). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200. Counter stain: Dapi (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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